Analytical Variability Among Methods for the Measurement of 25-Hydroxyvitamin D

Abstract

Intermethod variability in 25(OH)D assays continues to limit our progress toward the establishment of reference values for 25(OH)D in health and our efforts to gain a better understanding of the role of vitamin D insufficiency as a risk factor for disease.

Performance evaluation of four 25-hydroxyvitamin D assays to measure 25-hydroxyvitamin D2

“…In the past, variability was reported in the ability of automated immunoassays to accurately measure serum 25(OH)D 2 concentrations [29][30][31][32]. The affinity for 25(OH)D 2 and other vitamin D metabolites was found to differ between assays, which-depending on the assay-reportedly overestimated [29,30,32], or underestimated 25(OH)D 2 concentrations [29,30,32,33].…”

“…The affinity for 25(OH)D 2 and other vitamin D metabolites was found to differ between assays, which-depending on the assay-reportedly overestimated [29,30,32], or underestimated 25(OH)D 2 concentrations [29,30,32,33]. Assays reported to overestimate 25(OH)D 2 concentrations included Siemens ADVIA Centaur® Vitamin D Total (Siemens, Tarrytown, NY, USA) [29,30,32].…”

Analytical and clinical performance of the new Fujirebio 25-OH vitamin D assay, a comparison with liquid chromatography-tandem mass spectrometry (LC-MS/MS) and three other automated assays

“…This study adds to a growing literature [9][10][11][12][13][14][15][16][17][22][23][24][25][26][27][28] [13,15,23]. In the accuracy performance testing using Labquality reference serum panel, both Roche and Abbott displayed a similar bias at higher 25(OH)D concentrations as with study serum samples.…”

“…Automated assay systems have been developed by a number of manufacturers. However, studies have shown significant variability, both between assays and between laboratories, with these systems [9,10]. Such variability makes the reliability of clinical decisions based on absolute cut-off values questionable.…”

Increased matrix metalloproteinase-9 in male elderly with low 25-hydroxy-vitamin D

“…(13,14) The 25 (OH)D is a difficult analyte to measure, since it is a hydrophobic molecule with several molecular forms and is strongly bound to vitamin D-binding protein (VDBP) and since there are no standardized reference materials and measurement procedures for 25(OH)D assessment. (18,21,22) The study of Green et al (23) showed a prevalence of 63% for vitamin D deficiency in 18-40 year old females in Jakarta. Goswami et al (24) reported a prevalence of up to 90% for hypovitaminosis D in India.…”