Anaplasma marginale major surface protein 1a: A marker of strain diversity with implications for control of bovine anaplasmosis

Cabezas-Cruz, Alejandro; Fuente, José de la

doi:10.1016/j.ttbdis.2015.03.007

Cited by 24 publications

(12 citation statements)

References 50 publications

(78 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Antigens from TBP such as Borrelia burgdorferi [67], flaviviruses [68], Ehrlichia chaffeensis [69], Anaplasma phagocytophilum [69][70][71] and Anaplasma marginale [72][73][74][75][76][77][78] among others have been proposed as candidate protective antigens for the control of pathogen infection and transmission. The possibility of combining these pathogen-derived antigens with tick-protective antigens should result in new vaccines for the control of vectorborne diseases (FIGURE 1).…”

Section: Tick Vaccines For the Control Of Pathogen Infection And Transmmentioning

confidence: 99%

Tick vaccines: current status and future directions

Fuente

Contreras

2015

Expert Review of Vaccines

Self Cite

111

117

View full text Add to dashboard Cite

Ticks and tick-borne diseases are a growing problem affecting human and animal health worldwide. Traditional control methods, based primarily on chemical acaricides, have proven not to be sustainable because of the selection of acaricide-resistant ticks. Tick vaccines appear to be a promising and effective alternative for control of tick infestations and pathogen transmission. The purpose of this review is to summarize previous tick vaccine development and performance and formulate critical issues and recommendations for future directions for the development of improved and effective tick vaccines. The development of effective screening platforms and algorithms using omics approaches focused on relevant biological processes will allow the discovery of new tick-protective antigens. Future vaccines will likely combine tick antigens with different protective mechanisms alone or pathogen-derived antigens. The application of tick vaccines as a part of integrated control strategies will ultimately result in the control of tick-borne diseases.

show abstract

Section: Tick Vaccines For the Control Of Pathogen Infection And Transmmentioning

confidence: 99%

Tick vaccines: current status and future directions

Fuente

Contreras

2015

Expert Review of Vaccines

Self Cite

111

117

View full text Add to dashboard Cite

show abstract

“…The msp1a and msp4 genes have been used as constant genetic marker for the differentiation of A. marginale strains (de la Fuente et al 2001; Kocan et al 2010 b ). The Msp1a genes do not provide phylogenetic information, whereas msp4 provide information (Kocan et al 2010 a ; Cabezas-Cruz and de la Fuente, 2015). Major surface protein 5 is highly conserved in Anaplasma species and is being effectively used as diagnostic antigen for serodiagnosis in competitive ELISA (cELISA; Torioni de Echaide et al 1998).…”

Section: Anaplasma Marginalementioning

confidence: 99%

Alpha proteobacteria of genusAnaplasma(Rickettsiales: Anaplasmataceae): Epidemiology and characteristics ofAnaplasmaspecies related to veterinary and public health importance

Atif

2016

Parasitology

View full text Add to dashboard Cite

The Anaplasma species are important globally distributed tick-transmitted bacteria of veterinary and public health importance. These pathogens, cause anaplasmosis in domestic and wild animal species including humans. Rhipicephalus, Ixodes, Dermacentor and Amblyomma genera of ticks are the important vectors of Anaplasma. Acute anaplasmosis is usually diagnosed upon blood smear examination followed by antibodies and nucleic acid detection. All age groups are susceptible but prevalence increases with age. Serological cross-reactivity is one of the important issues among Anaplasma species. They co-exist and concurrent infections occur in animals and ticks in same geographic area. These are closely related bacteria and share various common attributes which should be considered while developing vaccines and diagnostic assays. Movement of susceptible animals from non-endemic to endemic regions is the major risk factor of bovine/ovine anaplasmosis and tick-borne fever. Tetracyclines are currently available drugs for clearance of infection and treatment in humans and animals. Worldwide vaccine is not yet available. Identification, elimination of reservoirs, vector control (chemical and biological), endemic stability, habitat modification, rearing of tick resistant breeds, chemotherapy and tick vaccination are major control measures of animal anaplasmosis. Identification of reservoirs and minimizing the high-risk tick exposure activities are important control strategies for human granulocytic anaplasmosis.

show abstract

“…Currently, the identification and characterization of A. ovis mainly relies on the analysis of 16S rRNA and msp4 genes; however, these genes are highly conserved among heterologous strains [ 3 , 13 ]. In previous reports, the major surface protein 1a (Msp1a), encoded by the msp1a gene, has been recognized as a stable molecular marker for classifying strains of A. marginale [ 14 ]. It has been revealed that A. marginale Msp1a could have evolved on the strength of immune selection pressure and differs among strains due to variable sequences and numbers of tandem amino acid repeats located in the N-terminal region of the protein [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Characterization of Anaplasma ovis strains using the major surface protein 1a repeat sequences

et al. 2017

View full text Add to dashboard Cite

Background Anaplasma ovis is one of the tick-transmitted pathogens of small ruminants. It causes ovine anaplasmosis and widely distributed in the world. In contrast to extensive worldwide genetic diversity of A. marginale and A. phagocytophilum, there are few reports on the classification of A. ovis strains. This study was conducted to investigate the occurrence and characterize A. ovis strains from goats and sheep from 12 provinces in China.MethodsThe occurrence of A. ovis DNA was tested in 552 goats and sheep, by PCR based on the msp4 gene. Positive samples were used for the amplification of the msp1a gene of A. ovis. The Msp1a amino acid repeats were further identified and used for the characterization of A. ovis strains.ResultsThe results showed that 79 (14.3%) goats and sheep were positive for A. ovis. The infection rates of A. ovis among different study sites ranged from 0 to 100%, and were significantly higher in sheep (26.6%, 45/169) than in goats (8.9%, 34/383) (χ 2 = 21.403, df = 1, P < 0.001). The msp4 gene sequences of these isolates were 99.8–100% identical to each other, and they represented two sequence types. Forty-four partial msp1a gene sequences containing the repeat sequences were obtained from A. ovis-positive samples. After translation to amino acid sequences, 24 Msp1a repeats with 33 to 47 amino acids, which corresponded to 19 genotypes of A. ovis, were recognized in goats and sheep in China.Conclusions Anaplasma ovis is widely distributed in the investigated geographical regions. The msp4 gene of A. ovis had high sequence identity and was unable to be used to discriminate different strains. The Msp1a could be used as a genetic marker for characterizing A. ovis, and 19 genotypes of A. ovis were recognized in domestic small ruminants in China. The present study revealed, for the first time, the genetic diversity of A. ovis based on the analyses of Msp1a amino acid repeats.

show abstract

Anaplasma marginale major surface protein 1a: A marker of strain diversity with implications for control of bovine anaplasmosis

Cited by 24 publications

References 50 publications

Tick vaccines: current status and future directions

Tick vaccines: current status and future directions

Alpha proteobacteria of genusAnaplasma(Rickettsiales: Anaplasmataceae): Epidemiology and characteristics ofAnaplasmaspecies related to veterinary and public health importance

Characterization of Anaplasma ovis strains using the major surface protein 1a repeat sequences

Contact Info

Product

Resources

About