Ancient signal for nitrogen status sensing in the green lineage: Functional evidence of CDPK repertoire in Ostreococcus tauri

Caló, Gonzalo; Scheidegger, Dana; Martínez-Noël, Giselle; Salerno, Graciela L.

doi:10.1016/j.plaphy.2017.07.009

Cited by 10 publications

(4 citation statements)

References 51 publications

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“…Recently, CDPK s have been demonstrated to be related to N deficiency. The transcription levels of three OtCDPK s were upregulated by N depletion in marine green algae Ostreococcus tauri (Caló et al, 2017), and the mRNA levels of CDPK1 and CDPK3 from C. reinhardtii showed over 10-fold increase after 1 h of N starvation, followed by a decrease from 3 to 18 h (Motiwalla et al, 2014). These two C. reinhardtii CDPKs corresponded to CrCDPK8 and CrCDPK9 in our study.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification of Calcium-Dependent Protein Kinases in Chlamydomonas reinhardtii and Functional Analyses in Nitrogen Deficiency-Induced Oil Accumulation

Fei

Dai

et al. 2019

Front. Plant Sci.

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Calcium-dependent protein kinases (CDPKs) are recognized as important calcium (Ca2+) sensors in signal transduction and play multiple roles in plant growth and developmental processes, as well as in response to various environmental stresses. However, little information is available about the CDPK family in the green microalga Chlamydomonas reinhardtii. In this study, 15 CrCDPK genes were identified in C. reinhardtii genome, and their functions in nitrogen (N) deficiency-induced oil accumulation were analyzed. Our results showed that all CrCDPK proteins harbored the typical elongation factor (EF)-hand Ca2+-binding and protein kinase domains. Phylogenetic analysis revealed that these CrCDPKs were clustered into one group together with a subclade of several CPKs from Arabidopsis and rice, clearly separating from the remaining AtCPKs and OsCPKs. These genes were located in 10 chromosomes and one scaffold of C. reinhardtii and contained 6–17 exons. RNA sequencing and quantitative reverse transcription (qRT)-PCR assays indicated that most of these CrCDPKs were significantly induced by N deficiency and salt stress. Lanthanum chloride (LaCl3), a plasma membrane Ca2+ channel blocker, limited oil accumulation in C. reinhardtii under N-deficient conditions, suggesting that Ca2+ was involved in N deficiency-induced oil accumulation. Furthermore, RNA interference (RNAi) silencing analyses demonstrated that six CrCDPKs played positive roles and three CrCDPKs played negative roles in N deficiency-induced oil accumulation in C. reinhardtii.

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Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification of Calcium-Dependent Protein Kinases in Chlamydomonas reinhardtii and Functional Analyses in Nitrogen Deficiency-Induced Oil Accumulation

Fei

Dai

et al. 2019

Front. Plant Sci.

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“…This is striking, because it is not related to the nitrogen sources available in the medium. In addition, none of the 3 cyclin-dependent protein kinase genes shown to be involved in N assimilation responses ( 62 ) showed differential expression, suggesting that this response is not functioning. There is an adequate level of nitrate in the culture medium used (no ammonium provided in L1; see Materials and Methods), so nitrate uptake and nitrate reductase genes should be highly active, as they are in the control.…”

Section: Discussionmentioning

confidence: 99%

Prasinovirus Attack of Ostreococcus Is Furtive by Day but Savage by Night

Derelle

Yau

Moreau

et al. 2018

J Virol

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Prasinoviruses are large DNA viruses that infect diverse genera of green microalgae worldwide in aquatic ecosystems, but molecular knowledge of their life cycles is lacking. Several complete genomes of both these viruses and their marine algal hosts are now available and have been used to show the pervasive presence of these species in microbial metagenomes. We have analyzed the life cycle of Ostreococcus tauri virus 5 (OtV5), a lytic virus, using transcriptome sequencing (RNASeq) from 12 time points of healthy or infected Ostreococcus tauri cells over a day/ night cycle in culture. In the day, viral gene transcription remained low while host nitrogen metabolism gene transcription was initially strongly repressed for two successive time points before being induced for 8 h, but during the night, viral transcription increased steeply while host nitrogen metabolism genes were repressed and many host functions that are normally reduced in the dark appeared to be compensated either by genes expressed from the virus or by increased expression of a subset of 4.4% of the host's genes. Some host cells underwent lysis progressively during the night, but a larger proportion were lysed the following morning. Our data suggest that the life cycles of algal viruses mirror the diurnal rhythms of their hosts.IMPORTANCE Prasinoviruses are common in marine environments, and although several complete genomes of these viruses and their hosts have been characterized, little is known about their life cycles. Here we analyze in detail the transcriptional changes occurring over a 27-h-long experiment in a natural diurnal rhythm, in which the growth of host cells is to some extent synchronized, so that host DNA replication occurs late in the day or early in the night and cell division occurs during the night. Surprisingly, viral transcription remains quiescent over the daytime, when the most energy (from light) is available, but during the night viral transcription activates, accompanied by expression of a few host genes that are probably required by the virus. Although our experiment was accomplished in the lab, cyclical changes have been documented in host transcription in the ocean. Our observations may thus be relevant for eukaryotic phytoplankton in natural environments.

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“…This is striking, because it is not related to the nitrogen sources available in the medium. In addition, the none of the 3 cyclin-dependent protein kinase genes shown to be involved N assimilation responses (62) showed differential expression, suggesting that this response is not functioning. There is an adequate level of nitrate in the culture medium used, (no ammonium provided in L1, see "Methods"), so nitrate uptake and nitrate reductase genes should be highly active, as they are in the control.…”

Section: Nitrogen Assimilationmentioning

confidence: 99%

Prasinovirus attack of Ostreococcus is furtive by day but savage by night

Derelle¹,

Yau²,

Moreau³

et al. 2017

Preprint

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19Prasinoviruses are large DNA viruses that infect diverse genera of green microalgae 20 worldwide in aquatic ecosystems, but molecular knowledge of their life-cycles is lacking. 21Several complete genomes of both these viruses and their marine algal hosts are now 22 available and have been used to show the pervasive presence of these species in microbial 23 metagenomes. We have analysed the life-cycle of OtV5, a lytic virus, using RNA-Seq 24

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Ancient signal for nitrogen status sensing in the green lineage: Functional evidence of CDPK repertoire in Ostreococcus tauri

Cited by 10 publications

References 51 publications

Genome-Wide Identification of Calcium-Dependent Protein Kinases in Chlamydomonas reinhardtii and Functional Analyses in Nitrogen Deficiency-Induced Oil Accumulation

Genome-Wide Identification of Calcium-Dependent Protein Kinases in Chlamydomonas reinhardtii and Functional Analyses in Nitrogen Deficiency-Induced Oil Accumulation

Prasinovirus Attack of Ostreococcus Is Furtive by Day but Savage by Night

Prasinovirus attack of Ostreococcus is furtive by day but savage by night

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