And the beat goes on

Darby, Jack R. T.; Morrison, Janna L.

doi:10.1113/jp277026

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2019

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“…Bovine serum albumin (BSA; 2 mg/mL stock solution) was used to form a standard curve. Extracted protein samples (75 μg) were subject to sodium dodecyl surface (SDS) page and stained with Coomassie blue to determine equal protein loading .…”

Section: Methodsmentioning

confidence: 99%

Detecting metabolic differences in fetal and adult sheep adipose and skeletal muscle tissues

Darby

Sorvina

Bader

et al. 2019

Journal of Biophotonics

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The primary metabolic pathway required to produce ATP differs as a result of tissue type, developmental stage and substrate availability. We utilized molecular and histological techniques to define the metabolic status in foetal and adult, adipose and skeletal muscle tissues. Redox ratios of these tissues were also determined optically by two‐photon microscopy. Adult perirenal adipose tissue had a higher optical redox ratio than fetal perirenal adipose tissue, which aligned with glycolysis being used for ATP production; whereas adult skeletal muscle had a lower optical redox ratio than fetal skeletal muscle, which aligned with oxygen demanding oxidative phosphorylation activity being utilized for ATP production. We have compared traditional molecular and microscopy techniques of metabolic tissue characterization with optical redox ratios to provide a more comprehensive report on the dynamics of tissue metabolism.

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Section: Methodsmentioning

confidence: 99%