Andrographolide inhibits the tumour necrosis factor-α-induced upregulation of ICAM-1 expression and endothelial-monocyte adhesion

Abstract

Andrographolide, a diterpene isolated from Andrographis paniculata, has been shown to have several biological activities including analgesic, antipyretic and antiinflammatory effects. Since the upregulation of adhesion molecules expression and endothelial‐leucocytes adhesion are key steps in the development of inflammation, the present study examines whether andrographolide modulates these biological processes in vitro. Incubation of endothelial cells with non‐toxic concentrations (0.16–16.7 υg/mL) of andrographolide attenuated the tumour necrosis factor‐α (TNF)‐induced intercellular adhesion molecule‐1 (ICAM‐1) expression. Similar concentration ranges of andrographolide also inhibited the TNF‐induced endothelial‐monocyte adhesion in a concentration‐dependent manner. These effects of andrographolide may account for its reported in vivoantiinflammatory activity. © 1998 John Wiley & Sons, Ltd.

Andrographolide Interferes with T Cell Activation and Reduces Experimental Autoimmune Encephalomyelitis in the Mouse

“…1E). These data are supported by measurements of mitochondrial function showing that concentrations up to 50 M andrographolide did not affect cell viability (Habtemariam, 1998;Chiou et al, 2000).…”

Potential anti-inflammatory bioactives from medicinal plants of Western Ghats, India

“…A significant decrease in the levels of NFkB mRNA by compounds 5, 11, 12, decreased levels of tumor necrosis factor (TNF)-a, IL-6, MIP-2 and nitric oxide (NO) by all the compounds was recorded. [40,41] Artocarpus heterophyllus Lam: A. heterophyllus is a large evergreen tree cultivated throughout Southeast Asia for its blocking PGs secretion resulting in reduced fever and pain of inflammation [22] . However, prolonged use of NSAIDS results in side effect viz., a tendency to develop side effects due to inhibition of constitutive COX-1 as well COX-2 induced during inflammation.…”

Pharmacological Aspects of Andrographis paniculata on Health and Its Major Diterpenoid Constituent Andrographolide

“…66,67) Andrographolide attenuated the TNF-α-induced intercellular adhesion molecule-1 (ICAM-1) expression and also inhibited the TNF-induced endothelial-monocyte adhesion; those were key steps in the development of inflammation. 68) An anti-inflammatory benefit of andrographolide by reduction of inducible nitric oxide synthase (iNOS) protein expression through prevention of the de novo protein synthesis and decreasing the protein stability via a post-transcriptional mechanism has been inferred. [7][8][9] Furthermore, andrographolide exerted anti-inflammatory effects by inhibiting nuclear factor (NF)-κB binding to DNA, and thus reducing the expression of proinflammatory proteins, such as cyclooxygenase-2 (COX-2).…”