Angiogenic properties of sustained release platelet-rich plasma: Characterization in-vitro and in the ischemic hind limb of the mouse

Abstract

PRP is a natural reserver of various growth factors that can be collected autologously and is costeffective. Thus for clinical use, no special considerations concerning antibody formation and infection risk are needed.Some clinical devices to automatically prepare PRP are available at present. PRP are consistently being used clinically inthe department of orthopedics and plastic surgery (oral, maxillary facial) for a long time. On the basis of researchevidence, some publications have reported positive results in either bone or soft tissue healing. However, some researchconcludes that there is no or little benefit from PRP. This is likely due to faster degradation of growth factors in PRP sincesome authors suggest using sustained release form of PRP to deliver optimal effect of PRP. Gelatin hydrogel is also beingused clinically as a slow, sustained release of carrier for growth factors in our center recently.

Enhanced skin wound healing by a sustained release of growth factors contained in platelet-rich plasma

“…Granulation tissue contains macrophages that provide GFs necessary for fibroplasia and angiogenesis, which in turn provide new ECM for cell ingrowth and supply oxygen and nutrients for cell metabolism (Singer and Clark, 1999). The enhanced neovascularization by sustained delivery of GFs contained in PRP has been also reported in a previous study demonstrating that sustained release of GFs in PRP using gelatin hydrogel enhances vessel density and perfusion in ischemic hind limbs in mice compared to treatment with PRP only (Bir et al, 2009). HCF-PRP group showed enhanced neovascularization in the repair tissues as compared to no treatment, F-PRP, and C-PRP groups (Figures 6 and 7).…”

“…PRP was isolated as previously described (Bir et al, 2009). The blood was centrifuged at 2,400 rpm for 10 min at room temperature.…”

“…PRP contains not only a number of GFs, including platelet-derived growth factor (PDGF), vascular endothelial growth factor (VEGF), transforming growth factor (TGF)-β, insulin-like growth factor (IGF), and fibroblast growth factor (FGF)-2, but also cytokines and inflammatory mediators (Rozman and Bolta, 2007). Unfortunately, the clinical evidence for PRP as a therapeutic agent is insufficient (Froum et al, 2002;Raghoebar et al, 2005) due to obstacles such as rapid washout (Tsay et al, 2005) and inactivation (Bir et al, 2009) of the critical proteins in PRP. However, there have been a few studies on the sustained delivery of GFs contained in PRP whose aims were to prevent initial washout and control the release of important proteins in PRP to the concerned area (Hokugo et al, 2005).…”

Is there any association between mycosis fungoides and Th-1, Th-2 cytokines and transforming growth factor-beta 1 gene polymorphisms?

“…The interaction between these bioactive proteins and receptors has a key role in the process of wound healing 7,8 . PRP is used in the treatment of chronic cutaneous and soft tissue ulcerations and especially in periodontal and oral surgery, maxillofacial surgery, orthopedic and traumatic surgery, cosmetic and plastic surgery, spinal surgery, heart by-pass surgery, and in burns [9][10][11][12][13][14][15][16][17][18][19][20][21][22][23] . The purpose of our study was to examine the histopathological effects of topical PRP and PPP treatments in wound healing on rats with partial thickness wounds.…”

Effects of platelet rich plasma on fascial healing in rats with fecal peritonitis

“…Autologous Platelet-rich plasma (PRP), recently, has become popular as a treatment modality in the areas of orthopedic and trauma surgery, spinal surgery, plastic-reconstructive surgery, oral and maxillofacial-dental surgery, ophthalmological surgery, heart by-pass surgery and burns [7][8][9] . It contains the growth factors; platelet-derived growth factor (PDGF), epidermal growth factor,fibroblast growth factor(FGF), transforming growth factor-β(TGF-β), insulin-like growth factor (IGF-I, IGF-II), endothelial cell growth factor and vascular endothelial growth factor (VEGF).…”