Angiotensin converting enzyme inhibitory peptide fractions from Tibet wild peach kernel protein hydrolysates

Yang, You; Li, A.; Zhong, Zheng-Chang; Xie, Minhao

doi:10.1556/066.2019.48.4.11

Cited by 5 publications

(2 citation statements)

References 23 publications

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“…In traditional Chinese medicine, peach kernel was used to treat hypertension (Wang et al, 2013). Our previous study presented that a component isolated from Tibet peach kernels by alkaline proteinase hydrolyzation has a good ACE inhibiting activity (Yang et al, 2019). Considering that the targeting sites of different enzymes to the same protein are different, the hydrolysates from a protein treated by different enzymes are not same (Wang et al, 2021;Bougatef et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Isolation, purification and bioactivity of ACE inhibitory peptides from peach kernel protein enzymatic hydrolysate

Wang

Zhong

et al. 2022

Food Sci. Technol

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Peaches mainly produced in China are a source of food-derived proteins, but much of it is wasted. To make full use of the peaches and peaches related resources, we extracted proteins, including albumin, globulin, gliadin, and glutelin, from defatted Tibet wild peach kernels using Osborne method to study their angiotensin I-converting enzyme (ACE) inhibiting activity, which is an active ingredient for hypertension treatment. The different enzymatic products of these extracts were further separated by ultrafiltration membranes and reversed phase high-performance liquid chromatography (RP-HPLC). The component K2 originated from globulin hydrolysate by alcalase treatment showed the highest ACE inhibiting activity (IC 50 0.78 mg/mL). Through further analyzing the Mass spectrometry results of K2 and searching the BIOEP database, the specific dipeptide AH (consisted of Ala and His) has been identified as the ACE inhibitory peptide, providing a potential for using Tibet peach kernelderived peptides as an ingredient in functional food to control hypertension.

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Section: Introductionmentioning

confidence: 99%

Isolation, purification and bioactivity of ACE inhibitory peptides from peach kernel protein enzymatic hydrolysate

Wang

Zhong

et al. 2022

Food Sci. Technol

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“…As a sort of common but serious chronic disease, hypertension is associated with the high risks of cerebrovascular, cardiovascular and renal complications, affecting approximately 25% of adults worldwide at present time and this portion is estimated to 60% by 2025 (García-Mora et al, 2017;Ngo et al, 2015;Yang et al, 2019). Therefore, it is imperative to carry out the prevention and treatment of hypertension.…”

Section: Introductionmentioning

confidence: 99%

ACE-inhibitory peptides from Laminaria japonica and their potential anti-hypertensive mechanism

Wang

et al. 2021

CyTA - Journal of Food

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Bioactive peptides have been extensively applied for developing health-promoting foods. To improve the value of Laminaria japonica proteins (LJPs), anti-hypertensive peptides were prepared by using controlled enzymatic hydrolysis along with response surface methodology to improve their yields. Eight anti-hypertensive peptides with tyrosine (Tyr) at the C-terminal were determined through an efficient RP-HPLC method and molecular docking was performed to reveal the underlying mechanisms behind their effectiveness of anti-hypertension. According to the results, the strongest activity of hydrolysates was achieved under enzyme ratio (papain: alcalase: trypsin) 1:2:1, pH 8.0, temperature 60°C, substrate concentration 1.7%, enzyme addition 4.4%, and hydrolysis time 5.1 h. Furthermore, molecular docking results displayed that anti-hypertensive peptides could bind with ACE active sites through forming a stable composite structure, thus causing a significant reduction to activities. These results demonstrated the potential applications of LJPs as a source of anti-hypertensive peptides and provided a reference for the industrialized anti-hypertensive productions. Péptidos inhibidores de la ECA de Laminaria japonica y su potencial mecanismo antihipertensivo RESUMENLos péptidos bioactivos han sido aplicados ampliamente para preparar alimentos que promueven la salud. A fin de mejorar el valor de las proteínas de Laminaria japonica (LJP), en este estudio se prepararon péptidos antihipertensivos utilizando hidrólisis enzimática controlada junto con una metodología de superficie de respuesta destinada a mejorar su rendimiento. Mediante la aplicación de un método eficiente de RP-HPLC, estos procedimientos permitieron identificar ocho péptidos antihipertensivos con tirosina (Tyr) en el extremo C terminal, realizándose un acoplamiento molecular para revelar los mecanismos subyacentes a su eficacia antihipertensiva. De acuerdo con los resultados, la mayor actividad de los hidrolizados se alcanzó con una proporción de enzimas (papaína: alcalasa: tripsina) de 1:2:1, pH 8.0, temperatura de 60°C, concentración de sustrato del 1.7%, adición de enzimas de 4.4% y tiempo de hidrólisis de 5.1 horas. Por otra parte, los resultados de acoplamiento molecular mostraron que los péptidos antihipertensivos pueden unirse a los sitios activos de la ECA mediante la formación de una estructura compuesta estable, lo que provoca una reducción significativa de actividades. Estos resultados dan cuenta de las aplicaciones potenciales de los LJP como fuente de péptidos antihipertensivos y proporcionan una referencia para la producción de antihipertensivos industrializados.

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Isolation of Prunus persica kernel protein and its physicochemical properties and aggregating performance modulated by heating and ultrasonication

Tao,

Ye,

Tang

et al. 2024

Sustainable Chemistry and Pharmacy

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Angiotensin converting enzyme inhibitory peptide fractions from Tibet wild peach kernel protein hydrolysates

Cited by 5 publications

References 23 publications

Isolation, purification and bioactivity of ACE inhibitory peptides from peach kernel protein enzymatic hydrolysate

Isolation, purification and bioactivity of ACE inhibitory peptides from peach kernel protein enzymatic hydrolysate

ACE-inhibitory peptides from Laminaria japonica and their potential anti-hypertensive mechanism

Isolation of Prunus persica kernel protein and its physicochemical properties and aggregating performance modulated by heating and ultrasonication

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