Animal Research Biosafety

Alderman, T. Scott; Carpenter, Calvin B.; McGirr, Rebecca

doi:10.1177/1535676018776971

Cited by 11 publications

(7 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The thermal cycler protocol was 25 °C primer annealing for 10 min, 42 °C reverse transcriptions for 15 min, 85 °C inactivations for 5 min, and a final hold at 4 °C. All laboratory operations followed the guidelines from the Veterinary Personal Biosecurity & Infection Control Handbook for biosecurity and infection control [ 31 ].…”

Section: Methodsmentioning

confidence: 99%

The Potential Protective Effect and Underlying Mechanisms of Physiological Unconjugated Hyperbilirubinemia Mediated by UGT1A1 Antisense Oligonucleotide Therapy in a Mouse Model of Cyclosporine A-Induced Chronic Kidney Disease

et al. 2022

View full text Add to dashboard Cite

Cyclosporine A (CSA) is an immunosuppressive drug that has improved transplant survival rates. However, its use is often limited because it is thought to be linked to the development of chronic kidney disease after kidney transplants. This study aimed to investigate the protective effects and underlying mechanisms of physiological unconjugated (UC) hyperbilirubinemia mediated by UGT1A1 antisense oligonucleotide in a mouse model of CsA-induced chronic kidney disease, and match these with that of chitosan (CH) as a natural chelator against kidney injury. In the current study, CsA-treated mice were given an intravenous injection of UGT1A1 antisense morpholino oligonucleotide (16 µg/kg) every third day for 14 days. In serum samples, bilirubin, creatinine, and urea were determined. Markers of oxidative stress, antioxidant activities, and mRNA expression of target genes PPAR-α, cFn, eNOS, NF-B, AT1-R, ETA-R, Kim-1, and NGAL were measured in the kidney tissues. Moreover, histopathological examinations were carried out on the kidney tissue. Physiological UC hyperbilirubinemia could be a promising protective strategy against CsA-induced kidney disease in transplant recipients. UGT1A1 antisense oligonucleotide-induced physiological UC hyperbilirubinemia serum significantly protected against CsA-induced kidney dysfunction. UCB acts as a signaling molecule that protects against kidney disease through different mechanisms, including antioxidant, anti-inflammatory, and hormonal action, by activating nuclear hormone receptors (PPAR-α). Moreover, it significantly downregulated mRNA expression of NF-kB, ETA-R, iNOS, AT1-R, cFn, Kim-1, and NGAL in the kidney tissue and alleviated CsA-induced kidney histological changes in CsA-treated mice.

show abstract

Section: Methodsmentioning

confidence: 99%

The Potential Protective Effect and Underlying Mechanisms of Physiological Unconjugated Hyperbilirubinemia Mediated by UGT1A1 Antisense Oligonucleotide Therapy in a Mouse Model of Cyclosporine A-Induced Chronic Kidney Disease

et al. 2022

View full text Add to dashboard Cite

show abstract

“…The Committee for Research Ethics of Mansoura University's Faculty of Veterinary Medicine in Egypt has accepted all experimental procedures (PhD/29). All the procedures of biosecurity and biosafety for research work in laboratories were followed [16]. The mice were purchased from a medical research center (MERC), Faculty of Medicine, Mansoura University, 35516, Mansoura, Egypt.…”

Section: Experimental Animalsmentioning

confidence: 99%

The Effects of Fructose on the Developmental Competence, Biochemical Biomarkers and Apoptotic Gene Expression by Murine Oocytes

Helmy

Halawa²,

Montaser

et al. 2023

Egyptian Journal of Veterinary Sciences

View full text Add to dashboard Cite

T HE goal of the current study was to investigate the effects of fructose supplementation in the culture medium on in vitro maturation, and the developmental competence of murine oocytes, biochemical markers and the expression of apoptotic and HIF-1α in cumulus oocyte cells (COCs). Murine oocytes were matured in IVF media supplemented with 1.25, 2.75 and 5-mM fructose. The developmental parameters (MI, MII, GVBD, degenerated and activated cells) were examined with NO, GPX, GSH, MDA, and TAC biomarkers. The expression of mRNA for apoptotic genes by COCs were evaluated. The low dosage improves significantly (P<0.05) the developmental competence markers than the control and higher doses of fructose. In vitro-matured mouse oocytes with 1.25 mM fructose showed a significant increase (P<0.05) in NO level in the In-vitro matured when compared with the control and other groups. The GPX concentrations in the in-vitro matured oocytes showed a significant decrease (P<0.05) with 5mM fructose. The MDA levels in the vitro-matured oocytes were higher (P<0.05) when the murine oocytes were exposed to 2.75 mM of fructose. The TAC in the oocyte showed no changes (P˂0.05) when the oocytes were exposed to both 1.25 mM. Moreover, the supplementation of IVM media showed a down regulation of apoptotic and HIF-1α genes when compared with the control and high dosage supplementation (P<0.05). Altogether, the supplementation of IVM media with lower dosage of fructose improves the developmental competence markers in the murine oocytes via its effects on oxidant and antioxidant activity and downregulation of apoptotic and HIF-1α genes.

show abstract

“…The bodies of the other euthanized rats were discarded using the technique outlined before. 19 For comparison with silver ion, we also give data from a previously published article on nano-silver treatments of postnatal models at a dose of 1/50 of LD50 equivalent to 100 mg/kg (group 5). 8…”

Section: Experimental Grouping and Designmentioning

confidence: 99%

Zinc Chloride Ameliorates the Adverse Effects of Silver Nitrates Compared to Silver Nanoparticle in Post-Natal Model of Toxicity.

Fehaid

Elazab

Elhadidy

et al. 2022

Mat. Sci. Res. India

View full text Add to dashboard Cite

Silver nanoparticles have been shown to increase postnatal toxicity in breastfeeding female rats, with negative consequences for their offspring. We wanted to investigate more about the differences in toxicity between silver nitrates and silver nanoparticles, as well as the impact of zinc chloride treatment on the silver nitrates induced toxicity on female albino rats. For 21 days, breastfeeding female albino rats and their puppies were exposed to silver nitrates at dosages of 0, 50, and 100 mg/kg. The results demonstrated that silver nitrates were more hazardous than nano-silver, as evidenced by higher free radical release, increased MDA levels, and decreased antioxidant enzyme levels (SOD). In addition, the silver ions-treated group had higher levels of liver enzymes and creatinine. Zinc chloride treatment, in particular, had a protective impact and mitigated the negative effects of silver nitrates, as seen by the restoration of baseline levels of liver enzyme, creatinine, and antioxidant enzyme. In addition, zinc chloride therapies reduced the harmful effects of silver nitrates on liver and kidney tissues but not lung tissue.

show abstract

Animal Research Biosafety

Cited by 11 publications

References 14 publications

The Potential Protective Effect and Underlying Mechanisms of Physiological Unconjugated Hyperbilirubinemia Mediated by UGT1A1 Antisense Oligonucleotide Therapy in a Mouse Model of Cyclosporine A-Induced Chronic Kidney Disease

The Potential Protective Effect and Underlying Mechanisms of Physiological Unconjugated Hyperbilirubinemia Mediated by UGT1A1 Antisense Oligonucleotide Therapy in a Mouse Model of Cyclosporine A-Induced Chronic Kidney Disease

The Effects of Fructose on the Developmental Competence, Biochemical Biomarkers and Apoptotic Gene Expression by Murine Oocytes

Zinc Chloride Ameliorates the Adverse Effects of Silver Nitrates Compared to Silver Nanoparticle in Post-Natal Model of Toxicity.

Contact Info

Product

Resources

About