Animal species identification in food products: Evolution of biomolecular methods

Bottero, Maria Teresa; Dalmasso, Alessandra

doi:10.1016/j.tvjl.2010.09.024

Cited by 133 publications

(69 citation statements)

References 45 publications

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“…DNA characterized by more stability under intensive heating, pressures, and chemical processing, has conserved structure in whole body cells, has a great identification power since they are rely on the recognition of specific DNA segments sequence of a particular tissue or animal (Calvo et al, 2001;Frezza et al, 2003;Girish et al, 2004;Lanzilao et al, 2005;Akasaki et al, 2006;Arslan et al, 2006;Rashid et al, 2014). From DNAbased techniques, polymerase chain reaction (PCR) is the most employed, simple, time saving, sensitive and specific method that could identify the species of origin exposed to different processing conditions (Mafra et al, 2008;Bottero and Dalmasso, 2011;Floren et al, 2015). In addition, the use of PCR in food analysis has provided various analytical methods for rapid detection and identification at species and intra-species level; however DNA-based methods still face some important limitations especially for quantitative measurements of food composition (Woolfe and Primrose, 2004).…”

Section: Advances In Animal and Veterinary Sciencesmentioning

confidence: 99%

Identification of Different Animal Species in Meat and Meat Products: Trends and Advances

Farag¹,

Alagawany²,

El‐Hack³

et al. 2015

Adv. Anim. Vet. Sci.

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| Identification of animal species of origin in meat and meat products is a matter of great concerns such as religious, economical, legal as well as medical aspects. Thus, several analytical techniques have been suggested for the identification of meat species either in individual or in mixed samples to protect consumers from the fraudulent and bad habits of marketing. DNA-based techniques especially the techniques based on polymerase chain reaction (PCR) are recognized as the most appropriate methods employed for species identification in raw and processed meat. PCR techniques including randomly amplified polymorphic DNA (PCR-RAPD), restriction fragment length polymorphism (PCR-RFLP), PCR with species-specific primers, real-time PCR and PCR-nucleotide sequencing allow identification of meat species under different processing conditions. But the variability of DNA content on the level of species as well as target tissue make the DNA-based methods somewhat unsuitable for the quantification of exact percentages of different species in meat and meat products. For these reasons the proteomic approaches depending on identification of different peptide biomarkers has been developed and employed to give information on the different composition of food. To broad the knowledge about these technologies, this review is compiled in an attempt to provide an overview of the possible PCR-based analytical techniques that could help in identifying the meat species of origin in meat and meat products and threw the light on the identification of species specific peptide biomarkers by proteomic technologies as a new and attractive alternative that could overcome some of the limitations that faced DNAbased methods especially when used for meat exposed to intensive heating of processing as well as for meat mixtures.

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Section: Advances In Animal and Veterinary Sciencesmentioning

confidence: 99%

Identification of Different Animal Species in Meat and Meat Products: Trends and Advances

Farag¹,

Alagawany²,

El‐Hack³

et al. 2015

Adv. Anim. Vet. Sci.

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“…Microsatellite markers had been widely investigated for many applications such as genetic identification, assessment of parentage, breed assignment tests and traceability (Dalvit et al, 2007;Fernández et al, 2013;Heaton et al, 2002;Orrù, Napolitano, Catillo, & Moioli, 2006;Rosa, Sardina, Mastrangelo, Tolone, & Portolano, 2013;Tolone, Mastrangelo, Rosa, & Portolano, 2012). DNA analysis furnished different levels of identification: individual one is useful for safeguarding public and animal health and providing safe products for both domestic and export consumption (considering also that national disease monitoring depends on correct animal identification), while breed and species discrimination are interesting to detect fraud and to protect and valorize typical products (Bottero & Dalmasso, 2011;Cunningham & Meghen, 2001;Dalvit, De Marchi, Targhetta, Gervaso, & Cassandro, 2008). Breed genetic traceability allowed the assignment or exclusion of the breed of origin to a product.…”

Section: Introductionmentioning

confidence: 99%

Application of microsatellite markers as potential tools for traceability of Girgentana goat breed dairy products

Sardina

Tortorici

Mastrangelo

et al. 2015

Food Research International

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In livestock, breed assignment may play a key role in the certification of products linked to specific breeds. Traceability of farm animals and authentication of their products can contribute to improve breed profitability and sustainability of animal productions with significant impact on the rural economy of particular geographic areas and on breed and biodiversity conservation. With the goal of developing a breed genetic traceability system for Girgentana dairy products, the aim of this study was to identify specific microsatellite markers able to discriminate among the most important Sicilian dairy goat breeds, in order to detect possible adulteration in Girgentana dairy products. A total of 20 microsatellite markers were analyzed on 338 individual samples from Girgentana, Maltese, and Derivata di Siria goat breeds. Specific microsatellite markers useful for traceability of dairy products were identified. Eight microsatellite markers showed alleles present at the same time in Maltese and Derivata di Siria and absent in Girgentana and, therefore, they were tested on DNA pools of the three breeds. Considering the electropherograms' results, only FCB20, SRCRSP5, and TGLA122 markers were tested on DNA samples extracted from cheeses of Girgentana goat breed. These three microsatellite markers could be applied in a breed genetic traceability system of Girgentana dairy products in order to detect adulteration due to Maltese and Derivata di Siria goat breeds.

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“…The detection of multiple genetically engineered crops [64] is still an important topic in which the effect of food processing on plant DNA degradation and PCR-based analysis of transgenic foods has also been studied [65]. These DNA-based methods also allow the authenticity determination of meat and meat products [66], the identification of animal species in food products [67], as well as the authentication of maize [68].…”

Section: Food Analysis: Current State Of the Art Methodologies And mentioning

confidence: 99%

Food Analysis: Present, Future, and Foodomics

Cifuentes

2012

ISRN Analytical Chemistry

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This paper presents a revision on the instrumental analytical techniques and methods used in food analysis together with their main applications in food science research. The present paper includes a brief historical perspective on food analysis, together with a deep revision on the current state of the art of modern analytical instruments, methodologies, and applications in food analysis with a special emphasis on the works published on this topic in the last three years (2009)(2010)(2011). The article also discusses the present and future challenges in food analysis, the application of "omics" in food analysis (including epigenomics, genomics, transcriptomics, proteomics, and metabolomics), and provides an overview on the new discipline of Foodomics.

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Animal species identification in food products: Evolution of biomolecular methods

Cited by 133 publications

References 45 publications

Identification of Different Animal Species in Meat and Meat Products: Trends and Advances

Identification of Different Animal Species in Meat and Meat Products: Trends and Advances

Application of microsatellite markers as potential tools for traceability of Girgentana goat breed dairy products

Food Analysis: Present, Future, and Foodomics

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