Annular Repair Using High-Density Collagen Gel

Grunert, Peter; Borde, Brandon; Hudson, Katherine; Macielak, M.; Bonassar, Lawrence J.; Härtl, Roger

doi:10.1097/brs.0000000000000103

Cited by 54 publications

(52 citation statements)

References 31 publications

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“…35,36 Investigations into the potential benefit of annular repair at the time of discectomy are also an active area of research. Grunert et al 42 utilized high-density collagen (HDC) gel in rat intervertebral disc after creating an iatrogenic needle puncture defect. Through MRI and histology, the HDC gel applied was shown to help form a fibrous tissue covering the needle puncture; directly retaining nuclear tissue from extruding and restoring partial mechanical integrity to the annular membrane.…”

Section: Annular Repairmentioning

confidence: 99%

Recurrent disc herniation: Etiology, evaluation, treatment, outcomes, and prevention (annular repair)

Khalsa

Amorosa

2016

Seminars in Spine Surgery

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Section: Annular Repairmentioning

confidence: 99%

Recurrent disc herniation: Etiology, evaluation, treatment, outcomes, and prevention (annular repair)

Khalsa

Amorosa

2016

Seminars in Spine Surgery

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“…Although no further sign of disc degeneration was observed in terms of hydration, as evaluated using MRI and disc height measurements post-implantation, incomplete AF tissue regeneration was observed in the inner region of the newly formed tissue, combined with a fibrous capsule in the outer part with dissimilarity in terms of extracellular matrix (ECM) composition compared to native AF tissue. (13) In an attempt to fill AF defects and to promote tissue repair, we have previously reported the development of injectable porous scaffolds fabricated through the freeze drying of covalently crosslinked alginate based on carbodiimide chemistry. (16) Such scaffolds exhibit high pore interconnectivity and shape-memory properties ideal for this specific application, as it can expand and fill defects once hydrated.…”

Section: Introductionmentioning

confidence: 99%

“…(11) For this reason, extensive research involving injectablebiomaterial candidates for NP regeneration have been explored, but surprisingly a limited number of similar systems have been targeted for annulus fibrosus tissue to date. (12)(13)(14)(15)(16) High-density injectable collagen gels have been investigated to fill punctured AF tissue in a rat tail model. Although no further sign of disc degeneration was observed in terms of hydration, as evaluated using MRI and disc height measurements post-implantation, incomplete AF tissue regeneration was observed in the inner region of the newly formed tissue, combined with a fibrous capsule in the outer part with dissimilarity in terms of extracellular matrix (ECM) composition compared to native AF tissue.…”

Section: Introductionmentioning

confidence: 99%

Enhancing cell migration in shape-memory alginate–collagen composite scaffolds: In vitro and ex vivo assessment for intervertebral disc repair

et al. 2014

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Lower lumbar disc disorders pose a significant problem in an ageing society with substantial socioeconomic consequences. Both inner tissue (nucleus pulposus, NP) and outer tissue (annulus fibrosus, AF) of the intervertebral disc (IVD) are affected by such debilitating disorders and can lead to disc herniation and lower back pain. In this study, we developed an alginate-collagen composite porous scaffold with shape-memory properties to fill defects occurring in AF tissue of degenerated IVDs, which has the potential to be administered using minimal invasive surgery. In the first part of this work we assessed how collagen incorporation on pre-formed alginate scaffolds influences the physical properties of the final composite scaffold. We also evaluated the ability of AF cells to attach, migrate and proliferate on the composite alginate-collagen scaffolds compared to control scaffolds (alginate only). In vitro experiments, performed in IVD-like microenvironmental conditions (low glucose and low oxygen concentrations), revealed that for alginate only scaffolds, AF cells agglomerated in clusters with limited infiltration and migration capacity. In comparison, for alginatecollagen scaffolds, AF cells readily attached and colonized constructs, while preserving their typical fibroblastic-like cell morphology with spreading behavior and intense cytoskeleton expression. In a second part of this study, we investigated the effects of alginate-collagen scaffold when seeded with bone marrow derived mesenchymal stem cells (MSCs). In vitro, we observed that alginate-collagen porous scaffolds supported cell proliferation and extracellular matrix (ECM) deposition (collagen type I); which secretion was amplified by the local release of TGF-ß3. In addition, when cultured in ex vivo organ defect model, alginatecollagen scaffolds maintained viability of transplanted MSCs for up to 5 weeks. Taken together, these findings illustrate the advantages of incorporating collagen as a means to enhance cell migration and proliferation in porous scaffolds which could be used to augment tissue repair strategies.

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“…There studies were motivated in part by previous work demonstrating the efficacy of injected collagen gels for repair of AF defects in vivo 32 . While promising, these previous studies did not address the extent to which these gels restored mechanical function immediately after injury.…”

Section: Discussionmentioning

confidence: 99%

“…Crosslinked gels slowed or prevented the onset and progression of degeneration in rat caudal IVDs as evidenced by higher disc heights and NP hydration than untreated discs for up to 5 weeks after treatment 32 . Furthermore, discs treated with crosslinked collagen gels maintained healthy disc phenotype as seen in histological sections.…”

Section: Introductionmentioning

confidence: 97%

Injectable, high‐density collagen gels for annulus fibrosus repair: Anin vitrorat tail model

Borde

Grunert

Härtl

et al. 2014

J Biomedical Materials Res

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A herniated intervertebral disc often causes back pain when disc tissue is displaced through a damaged annulus fibrosus. Currently the only methods available for annulus fibrosus repair involve mechanical closure of defect, which does little to address biological healing in the damaged tissue. Collagen hydrogels are injectable and have been used to repair annulus defects in vivo. In this study, high-density collagen hydrogels at 5, 10 and 15 mg/ml were used to repair defects made to intact rat caudal intervertebral discs in vitro. A group of gels at 15 mg/ml were also crosslinked with riboflavin at 0.03 mM, 0.07 mM or 0.10 mM . These crosslinked, high-density collagen gels maintained presence in the defect under loading and contributed positively to the mechanical response of damaged discs. Discs exhibited increases to 95% of undamaged effective equilibrium and instantaneous moduli as well as up to four fold decreases in effective hydraulic permeability from the damaged discs. These data suggest that high density collagen gels may be effective at restoring mechanical function of injured discs as well as potential vehicles for delivery of biological agents such as cells or growth factors that may aid in the repair of the annulus fibrosus.

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Annular Repair Using High-Density Collagen Gel

Cited by 54 publications

References 31 publications

Recurrent disc herniation: Etiology, evaluation, treatment, outcomes, and prevention (annular repair)

Recurrent disc herniation: Etiology, evaluation, treatment, outcomes, and prevention (annular repair)

Enhancing cell migration in shape-memory alginate–collagen composite scaffolds: In vitro and ex vivo assessment for intervertebral disc repair

Injectable, high‐density collagen gels for annulus fibrosus repair: Anin vitrorat tail model

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