Antennal lobe projection destinations of Helicoverpa zea male olfactory receptor neurons responsive to heliothine sex pheromone components

Lee, Seong Gyu; Carlsson, Mikael A.; Hansson, Bill S.; Todd, J. L.; Baker, Thomas C.

doi:10.1007/s00359-005-0071-8

Cited by 35 publications

(48 citation statements)

References 44 publications

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“…Thus, specific sensory neuron types, randomly distributed in the periphery, project to distinct addresses in the antennal lobe/olfactory bulb. These findings are in full agreement with results from tracing of physiologically characterized receptor neurons in several moth species (Hansson et al 1995;Berg et al 1998Berg et al , 2005Lee et al 2006).…”

Section: Introductionsupporting

confidence: 93%

Antennal-lobe tracts in the noctuid moth, Heliothis virescens: new anatomical findings

et al. 2016

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As in other insects, three main tracts in the moth brain form parallel connections between the antennal lobe and the protocerebrum. These tracts, which consist of the antennal-lobe projection-neuron axons, target two main areas in the protocerebrum, the calyces of the mushroom bodies and the lateral horn. In spite of the solid neuroanatomical knowledge already established, there are still unresolved issues regarding the antennal-lobe tracts of the moth. One is the proportion of lateral-tract neurons targeting the calyces. In the study presented here, we have performed both retrograde and anterograde labeling of the antennal-lobe projection neurons in the brain of the moth, Heliothis virescens. The results from the retrograde staining, obtained by applying dye in the calyces, demonstrated that the direct connection between the antennal lobe and this neuropil is maintained primarily by the medial antennal-lobe tract; only a few axons confined to the lateral tract were found to innervate the calyces. In addition, these staining experiments, which allowed us to explore the arborization pattern of labeled neurons within the antennal lobe, resulted in new findings regarding anatomical arrangement of roots and cell body clusters linked to the medial tract. The results from the anterograde staining, obtained by applying dye into the antennal lobe, visualized the total assembly of axons passing along the antennal-lobe tracts. In addition to the three classical tracts, we found a transverse antennal-lobe tract not previously described in the moth. Also, these staining experiments revealed an organized neuropil in the lateral horn formed by terminals of the four antennal-lobe tracts.

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Section: Introductionsupporting

confidence: 93%

Antennal-lobe tracts in the noctuid moth, Heliothis virescens: new anatomical findings

et al. 2016

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“…This type of ORN serves as a catch-all for three different behaviorally antagonistic pheromone-related compounds, including Z9-14:Ald, emitted by several non-conspecifi cs. At the same time, the second ORN in the H. zea C-type sensilla responsive to Z9-16:Ald and involved in attraction, also responds well to Z9-14:Ald and sends its axon to the dorso-medial glomerulus of the MGC involved in attraction [Lee et al, 2005]. Thus it may be that the same receptor is co-expressed with a different receptor on two different types of ORNs and could modulate male behavior in ways important to the evolution of sex pheromone blends by broadening the responsiveness of ORNs.…”

Section: Co-expression Of Two Odorant Receptors On the Same Orn Explamentioning

confidence: 99%

“…In some classes of sensilla (such as A-type), one of these co-compartmentalized ORNs is always completely silent to any tested odorants, whereas the other responds readily to a pheromone component. The presence of silent ORNs has been revealed by cobalt staining of pairs of ORNs in A-type sensilla of H. zea [Lee et al, 2005], H. subfl exa , and H. virescens [Berg et al, 1998]. Cobalt staining also revealed that a silent ORN is also present in the B-type sensilla of H. subfl exa [S.G. Lee et al, unpubl.…”

Section: Odorant Receptor Expression Dictates Orn Tuning Profi Lesmentioning

confidence: 99%

“…The very broadly tuned ORN in the C-type sensilla on H. zea antennae [Cossé et al, 1998] responding to three different behaviorally antagonistic ligands sends its axon to the antero-medial antagonist-related glomerulus of the MGC [Lee et al, 2005]. This type of ORN serves as a catch-all for three different behaviorally antagonistic pheromone-related compounds, including Z9-14:Ald, emitted by several non-conspecifi cs.…”

Section: Co-expression Of Two Odorant Receptors On the Same Orn Explamentioning

confidence: 99%

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Inheritance of Olfactory Preferences II. Olfactory Receptor Neuron Responses from <i>Heliothis subflexa</i> × <i>Heliothis virescens</i> Hybrid Male Moths

et al. 2006

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Single-cell electrophysiological recordings were obtained from olfactory receptor neurons (ORNs) in sensilla trichodea on male antennae of hybrids formed mainly by crossing female Heliothis subflexa with male Heliothis virescens (‘SV hybrids’). We recorded from the A-, B-, and C-type sensilla trichodea, with the latter two types housing ORNs exhibiting response profiles to different pheromone components that we had previously found to be characteristic for each species. For both the B- and the C-type SV hybrid sensilla, most of the ORNs exhibited a spike amplitude and ORN co-compartmentalization within sensilla that more strongly resembled the ORNs of parental H. subflexa rather than those of H. virescens. The overall mean dose-response profiles of the ORNs in hybrid C- and B-type sensilla were intermediate between those of the H. virescens and H. subflexa parental type ORNs. However, not all hybrid ORNs were intermediate in their tuning spectra, but rather ranged from those that closely resembled H. subflexa or H. virescens parental types to those that were intermediate, even on the same antenna. The most noteworthy shift in ORN responsiveness in hybrid males was an overall increase in sensitivity to Z9–14:Ald exhibited by Z9–16:Ald-responsive ORNs. Heightened cross-responsiveness to Z9–14:Ald by hybrid ORNs correlates well with observed behavioral cross-responsiveness of hybrids in which Z9–14:Ald could substitute for Z9–16:Ald in the pheromone blend, a behavior not observed in parental types. The hybrid ORN shifts involving greater sensitivity to Z9– 14:Ald also correlate well with studies of hybrid male antennal lobe interneurons that exhibited a shift toward greater cross-responsiveness to Z9–14:Ald and Z9– 16:Ald. We propose that the differences between parental H. virescens, H. subflexa, and SV hybrid male pheromone ORN responsiveness to Z9–16:Ald and Z9–14:Ald are most logically explained by an increased or decreased co-expression of two different odorant receptors for each of these compounds on the same ORN.

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“…Single sensillum recordings have shown that in most heliothine moths, Heliothis and Helicoverpa species, divergent types of sensilla house OSNs that are tuned to different principal sex pheromone components [9]–[14], [26]–[28]. Typically, a larger fraction of OSNs responds to the major components of sex pheromones in heliothine moths [11], [13], [14], [29], [30], possibly to increase the sensitivity for pheromones. Baker et al (2012) recently suggested that the functional reason for over-representation of OSNs for major pheromone components on moth antennae is to accommodate the greater dynamic flux rates encountered for the major pheromone component while maintaining overall response ratios [31].…”

Section: Introductionmentioning

confidence: 99%

Peripheral Coding of Sex Pheromone Blends with Reverse Ratios in Two Helicoverpa Species

Hou

Huang

et al. 2013

PLoS ONE

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The relative proportions of components in a pheromone blend play a major role in sexual recognition in moths. Two sympatric species, Helicoverpa armigera and Helicoverpa assulta, use (Z)-11-hexadecenal (Z11–16: Ald) and (Z)-9-hexadecenal (Z9–16: Ald) as essential sex pheromone components but in very different ratios, 97∶3 and 7∶93 respectively. Using wind tunnel tests, single sensillum recording and in vivo calcium imaging, we comparatively studied behavioral responses and physiological activities at the level of antennal sensilla and antennal lobe (AL) in males of the two species to blends of the two pheromone components in different ratios (100∶0, 97∶3, 50∶50, 7∶93, 0∶100). Z11–16: Ald and Z9–16: Ald were recognized by two populations of olfactory sensory neurons (OSNs) in different trichoid sensilla on antennae of both species. The ratios of OSNs responding to Z11–16:Ald and Z9–16:Ald OSNs were 100∶28.9 and 21.9∶100 in H. armigera and H. assulta, respectively. The Z11–16:Ald OSNs in H. armigera exhibited higher sensitivity and efficacy than those in H. assulta, while the Z9–16:Ald OSNs in H. armigera had the same sensitivity but lower efficacy than those in H. assulta. At the dosage of 10 µg, Z11–16: Ald and Z9–16: Ald evoked calcium activity in 8.5% and 3.0% of the AL surface in H. armigera, while 5.4% and 8.6% of AL in H. assulta, respectively. The calcium activities in the AL reflected the peripheral input signals of the binary pheromone mixtures and correlated with the behavioral output. These results demonstrate that the binary pheromone blends were precisely coded by the firing frequency of individual OSNs tuned to Z11–16: Ald or Z9–16: Ald, as well as their population sizes. Such information was then accurately reported to ALs of H. armigera and H. assulta, eventually producing different behaviors.

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Antennal lobe projection destinations of Helicoverpa zea male olfactory receptor neurons responsive to heliothine sex pheromone components

Cited by 35 publications

References 44 publications

Antennal-lobe tracts in the noctuid moth, Heliothis virescens: new anatomical findings

Antennal-lobe tracts in the noctuid moth, Heliothis virescens: new anatomical findings

Inheritance of Olfactory Preferences II. Olfactory Receptor Neuron Responses from <i>Heliothis subflexa</i> × <i>Heliothis virescens</i> Hybrid Male Moths

Peripheral Coding of Sex Pheromone Blends with Reverse Ratios in Two Helicoverpa Species

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