Anthracycline analysis by capillary electrophoresis

Quantitative liquid chromatographic analysis of anthracyclines in biological fluids

“…Although the focus of this review is on chromatographic techniques, it needs to be mentioned that also capillary electrophoresis with UV [84], amperometric [85] and laser-induced fluorescence detection [86][87][88][89][90][91][92] and other techniques [93,94] have been described. The majority of these methods have been reviewed elsewhere [16,17].…”

Capillary Electrophoresis with Laser-induced Fluorescence Detection for Application in Intracellular Investigation of Anthracyclines and Multidrug Resistance Proteins

“…A migration solution containing 30% (v/v) acetonitrile was used in a similar separation of DOX, IDA and DNR 39 using CE-LIF. Separations of DOX and DNR after extraction, were reported by Simeon et al 40 and Hempel et al 41 in the determination of anthracyclines in plasma and tumors. Similar work employing cyclodextrin (CD)-assisted MEKC on the separation of DOX and doxorubicinol was reported by Eder et al 42 It should be mentioned that the aforementioned CE methods used organic solvents, which are known to precipitate proteins in biological samples, either during separation or extraction.…”

Antineoplastic drugs and their analysis: a state of the art review

“…339 LC-ELSD was also applied for the analysis of daunorubicin and its degradation products for a stability study of injectable solution of anthracycline (0.25 mg mL −1 was the lowest concentration of standard solutions tested), but the interest of ELSD for anthracyclines is not obvious since these molecules can be easily detected by UV, FD and MS. 340 As already mentioned, CE was another separation technique widely used for the determination of anthracyclines. LIF detection [341][342][343][344][345][346][347][348][349] is usually preferred to UV detection 350 to improve sensitivity. However, the poor UV sensitivity observed in CE (due to the narrow optical path of the capillaries) can be compensated for by a sweeping preconcentration step and an electrokinetic injection.…”