Anti-acidogenic and anti-biofilm activity of 5,6,8-trihydroxy-7-methoxy-2-(4-methoxyphenyl)-4H-chromen-4-one

Ngabaza, Thamsanqa; Moeno, Sharon; Patel, Mrudula

doi:10.1016/j.micpath.2018.07.003

Cited by 9 publications

(3 citation statements)

References 16 publications

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“…Secondary metabolites like cytochalasins from ascomycetes reduced biofilm formation of S. aureus by 20-90% when applied at concentrations of 32-250 mg L −1 (Yuyama et al, 2018). A similar biofilm reduction of >90 was observed when ∼200 mg L −1 of secondary metabolites of the plant Dodonaea viscosa was added to S. mutans (Ngabaza et al, 2018). Functionalizing surfaces with biofilm-degrading enzymes such as glycoside hydrolase can reduce Pseudomonas aeruginosa biolayer formation by more than 99% (Asker et al, 2018).…”

Section: Degradation Of Experimental Biofilms Using Plant-derived Lg2 and Combinations Of Different Enzyme Classesmentioning

confidence: 72%

Expression of Biofilm-Degrading Enzymes in Plants and Automated High-Throughput Activity Screening Using Experimental Bacillus subtilis Biofilms

Opdensteinen

Dietz

Gengenbach

et al. 2021

Front. Bioeng. Biotechnol.

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Biofilm-forming bacteria are sources of infections because they are often resistant to antibiotics and chemical removal. Recombinant biofilm-degrading enzymes have the potential to remove biofilms gently, but they can be toxic toward microbial hosts and are therefore difficult to produce in bacteria. Here, we investigated Nicotiana species for the production of such enzymes using the dispersin B-like enzyme Lysobacter gummosus glyco 2 (Lg2) as a model. We first optimized transient Lg2 expression in plant cell packs using different subcellular targeting methods. We found that expression levels were transferable to differentiated plants, facilitating the scale-up of production. Our process yielded 20 mg kg−1 Lg2 in extracts but 0.3 mg kg−1 after purification, limited by losses during depth filtration. Next, we established an experimental biofilm assay to screen enzymes for degrading activity using different Bacillus subtilis strains. We then tested complex and chemically defined growth media for reproducible biofilm formation before converting the assay to an automated high-throughput screening format. Finally, we quantified the biofilm-degrading activity of Lg2 in comparison with commercial enzymes against our experimental biofilms, indicating that crude extracts can be screened directly. This ability will allow us to combine high-throughput expression in plant cell packs with automated activity screening.

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Section: Degradation Of Experimental Biofilms Using Plant-derived Lg2 and Combinations Of Different Enzyme Classesmentioning

confidence: 72%

Expression of Biofilm-Degrading Enzymes in Plants and Automated High-Throughput Activity Screening Using Experimental Bacillus subtilis Biofilms

Opdensteinen

Dietz

Gengenbach

et al. 2021

Front. Bioeng. Biotechnol.

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“…In addition, DVA-derived flavone also inhibits biofilm formation and acid production by cariogenic bacteria S. mutans . This means that DVA would reduce the Candida counts and improve the oral hygiene, which would further decrease the Candida counts [ 131 ]. Nevertheless, beneficial plant-derived compounds can be further explored for their cytotoxic effects and in vivo efficacy and developed into mouthrinses or oral gels [ 132 , 133 ].…”

Section: Prevention and Treatmentmentioning

confidence: 99%

Oral Cavity and Candida albicans: Colonisation to the Development of Infection

Patel

2022

Pathogens

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Candida colonisation of the oral cavity increases in immunocompromised individuals which leads to the development of oral candidiasis. In addition, host factors such as xerostomia, smoking, oral prostheses, dental caries, diabetes and cancer treatment accelerate the disease process. Candida albicans is the primary causative agent of this infection, owing to its ability to form biofilm and hyphae and to produce hydrolytic enzymes and candialysin. Although mucosal immunity is activated, from the time hyphae-associated toxin is formed by the colonising C. albicans cells, an increased number and virulence of this pathogenic organism collectively leads to infection. Prevention of the development of infection can be achieved by addressing the host physiological factors and habits. For maintenance of oral health, conventional oral hygiene products containing antimicrobial compounds, essential oils and phytochemicals can be considered, these products can maintain the low number of Candida in the oral cavity and reduce their virulence. Vulnerable patients should be educated in order to increase compliance.

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“…The effects of numerous natural agents on cariogenic bacteria have been investigated to determine their multiple bioactivities, less adverse effects and resistance problems 13) . Apigenin is chemically known as 4',5,7-trihydroxyflavone.…”

Section: Introductionmentioning

confidence: 99%

Effect of apigenin on surface-associated characteristics and adherence of <i>Streptococcus mutans </i>

Liu

Han²,

Yang

et al. 2020

Dent. Mater. J.

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Apigenin is a type of flavonols that exhibits anti-caries properties. Bacterial adherence is the initial step in the forming of a stable biofilm that leads to caries. Bacterial adherence is affected by surface characteristics, including hydrophobicity and bacterial aggregation. However, the effect of apigenin on surface characteristics of cariogenic bacteria has not been reported. We aimed to examine the effects of apigenin on adherence and biofilm formation of Streptococcus mutans UA159. Hydrophobicity and bacterial aggregation, pac and gbpC gene expressions, and cytotoxicity on human dental pulp cells were also determined. Apigenin significantly inhibited the adherence and biofilm formation of S. mutans. Hydrophobicity decreased, whereas the aggregation rate was significantly increased compared with the control. Apigenin significantly suppressed pac and gbpC gene expressions. Apigenin exhibited acceptable biocompatibility on hDPCs. Thus, apigeinin may affect adherence and biofilm formation by altering the surface properties of S. mutans without obvious adverse effect on hDPCs.

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Anti-acidogenic and anti-biofilm activity of 5,6,8-trihydroxy-7-methoxy-2-(4-methoxyphenyl)-4H-chromen-4-one

Cited by 9 publications

References 16 publications

Expression of Biofilm-Degrading Enzymes in Plants and Automated High-Throughput Activity Screening Using Experimental Bacillus subtilis Biofilms

Expression of Biofilm-Degrading Enzymes in Plants and Automated High-Throughput Activity Screening Using Experimental Bacillus subtilis Biofilms

Oral Cavity and Candida albicans: Colonisation to the Development of Infection

Effect of apigenin on surface-associated characteristics and adherence of <i>Streptococcus mutans </i>

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