Anti-adhesive antimicrobial peptide coating prevents catheter associated infection in a mouse urinary infection model

Yu, Kai; Lo, Joey; Mei, Yan; Yang, Xiaoqiang; Brooks, Donald E.; Hancock, Robert E. W.; Lange, Dirk; Kizhakkedathu, Jayachandran N.

doi:10.1016/j.biomaterials.2016.11.047

Cited by 220 publications

(184 citation statements)

References 53 publications

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“…Nevertheless, AMPs can be immobilized onto solid surfaces to enhance efficacy or specificity. This was particularly effective as a polymer-based approach on catheters, as AMP coatings greatly reduced P. aeruginosa adhesion and infection over 7 days in a mouse UTI model 107 . Furthermore, structurally nanoengineered AMP polymers exhibited potent killing activity against several Gram-negative, colistin-resistant and MDR pathogens, and they exhibited low toxicity and efficacy in an animal model of Acinetobacter baumannii infection 108 .…”

Section: Targeting Dormant Cells In Biofilmsmentioning

confidence: 99%

Targeting microbial biofilms: current and prospective therapeutic strategies

et al. 2017

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Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections. The multifactorial nature of biofilm development and drug tolerance imposes great challenges for the use of conventional antimicrobials, and indicates the need for multi-targeted or combinatorial therapies. In this review, we focus on current therapeutic strategies and those that are under development that target vital structural and functional traits of microbial biofilms and drug tolerance mechanisms, including the extracellular matrix and dormant cells. We emphasize strategies that are supported by in vivo or ex vivo studies, highlight emerging biofilm-targeting technologies, and provide a rationale for multi-targeted therapies that are aimed at disrupting the complex biofilm microenvironment.

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Section: Targeting Dormant Cells In Biofilmsmentioning

confidence: 99%

Targeting microbial biofilms: current and prospective therapeutic strategies

et al. 2017

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“…Biofilms on biomedical devices can be limited by coating the device surface with peptides [59]. Yu et al [60] tethered an AMP to polyurethane in a mouse catheter model infected with P. aeruginosa. The coating led to a 4 log-fold reduction in bacterial load compared to uncoated material and also reduced bacterial load in urine, without having toxic effects on eukaryotic cells.…”

Section: Formulation and Therapeutic Delivery Of Anti-biofilm Peptidesmentioning

confidence: 99%

Design and Assessment of Anti-Biofilm Peptides: Steps Toward Clinical Application

Dostert¹,

Belanger²,

Hancock³

2018

J Innate Immun

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Highly antibiotic resistant, microbial communities, referred to as biofilms, cause various life-threatening infections in humans. At least two-thirds of all clinical infections are biofilm associated, and antibiotic therapy regularly fails to cure patients. Anti-biofilm peptides represent a promising approach to treat these infections by targeting biofilm-specific characteristics such as highly conserved regulatory mechanisms. They are being considered for clinical application and we discuss here key factors in discovery, design, and application, particularly the implementation of host-mimicking conditions, that are required to enable the successful advancement of potent anti-biofilm peptides from the bench to the clinic.

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“…The tethered peptides on the PU catheter surface displayed broad spectrum antimicrobial activity and showed long term activity in vitro and in vivo. The AMP‐brush coating also showed good biocompatibility with bladder epithelial cells and fibroblast cells in cell culture …”

Section: Strategies For Developing Antimicrobial Catheter Surfacementioning

confidence: 98%

Biomodification Strategies for the Development of Antimicrobial Urinary Catheters: Overview and Advances

Anjum¹,

Singh²,

Lepoittevin

et al. 2017

Global Challenges

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Microbial burden associated with medical devices poses serious health challenges and is accountable for an increased number of deaths leading to enormous medical costs. Catheter‐associated urinary tract infections are the most common hospital‐acquired infections with enhanced patient morbidity. Quite often, catheter‐associated bacteriuria produces apparent adverse outcomes such as urosepsis and even death. Taking this into account, the methods to modify urinary catheters to control microbial infections with relevance to clinical drug resistance are systematically evaluated in this review. Technologies to restrict biofilm formation at initial stages by using functional nanomaterials are elucidated. The conventional methodology of using single therapeutic intervention for developing an antimicrobial catheter lacks clinically meaningful benefit. Therefore, catheter modification using naturally derived antimicrobials such as essential oils, curcumin, enzymes, and antimicrobial peptides in combination with synthetic antibiotics/nanoantibiotics is likely to exert sufficient inhibitory effect on uropathogens and is extensively discussed. Futuristic efforts in this area are projected here that demand clinical studies to address areas of uncertainty to avoid development of bacterial resistance to the new generation therapy with minimum discomfort to the patients.

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Anti-adhesive antimicrobial peptide coating prevents catheter associated infection in a mouse urinary infection model

Cited by 220 publications

References 53 publications

Targeting microbial biofilms: current and prospective therapeutic strategies

Targeting microbial biofilms: current and prospective therapeutic strategies

Design and Assessment of Anti-Biofilm Peptides: Steps Toward Clinical Application

Biomodification Strategies for the Development of Antimicrobial Urinary Catheters: Overview and Advances

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