Anti-aging Properties of Conditioned Media of Epidermal Progenitor Cells Derived from Mesenchymal Stem Cells

Abstract

IntroductionReduced number and activities of epidermal stem cells are related to the features of photoaged skin. It was reported that conditioned media from various stem cell cultures are capable of improving the signs of cutaneous aging. This work was performed to establish epidermal progenitor cells derived from mesenchymal stem cells, and to evaluate the anti-aging efficacy of its conditioned media.MethodsEpidermal progenitor cell culture was established by differentiation from mesenchymal stem cells, and its conditioned medium (EPC-CM) was prepared. Normal human dermal fibroblasts were exposed to hydrogen peroxide and the protective effects of EPC-CM were investigated, monitoring intracellular reactive oxygen species (ROS), cellular defense enzymes, collagen biosynthesis, and mitogen-associated protein kinase (MAPK) signaling. Anti-aging efficacy of cosmetic essence (5% EPC-CM) was evaluated by a clinical test with 25 Korean women aged between 29 and 69.ResultsHydrogen peroxide hindered proliferation of fibroblasts and increased the levels of intracellular ROS. Pretreatment of EPC-CM protected fibroblasts from oxidative stress as shown by accelerated proliferation and reduced ROS generation. EPC-CM effectively prevented hydrogen peroxide-induced alterations of the activities, as well as mRNA and protein levels, of antioxidative enzymes, such as superoxide dismutase, catalase, and glutathione peroxidase. Reduced type I collagen biosynthesis and stimulated phosphorylation of MAPK signaling proteins, induced by oxidative damage, were also prevented by EPC-CM. In clinical study, wrinkle, depression, and skin texture were improved by the topical application of a formulation containing 5% EPC-CM within 4 weeks.ConclusionEpidermal progenitor cell culture was established, and its conditioned medium was developed for anti-aging therapy. EPC-CM improved signs of skin aging in clinical study, possibly via activation of cellular the defense system, as supported by in vitro results.Electronic supplementary materialThe online version of this article (10.1007/s13555-018-0229-2) contains supplementary material, which is available to authorized users.

Anti-Wrinkle Benefits of Peptides Complex Stimulating Skin Basement Membrane Proteins Expression

“…Previously, we reported that the topical application of a conditioned media of epidermal progenitor cells derived from mesenchymal stem cells, which contained high levels of nidogen-1, significantly improved various skin aging parameters, such as wrinkle and skin texture, which is consistent with current result [34] In this study, the potential application of epidermal protein expression-modulating ingredients as anti-aging cosmeceuticals was investigated. Three peptide derivatives with stimulatory activities on collagen XVII, laminin and nidogen in vitro, were used in a mixed form, and their anti-aging activities were examined using ex vivo and clinical studies.…”

Human Mesenchymal Stromal Cells-Derived Conditioned Medium Based Formulation for Advanced Skin Care: in vitro and in vivo Evaluation

“…In order to validate secretome profiling of MSC derived CM, we initially performed proteomic profiling of CM using human specific ELISA kits and bead based multiplex method and the data of the same is already reported [10]. It is in corroboration with the cytokines/growth factors identified in the CM derived from MSC cultures published by other researchers [13,[16][17][18][19]. Since the aim of the present study was to evaluate anti-aging potential of human MSC derived CM formulation, we specifically selected two of the widely studied growth factors VEGF and TGF-β which are important for maintaining healthy skin.…”

“…Liu et al, showed that umbilical cord mesenchymal stem cells CM protects against photo aging induced by UVA and UVB radiation and is a promising candidate for skin anti-photo aging treatments [12]. Sohn et al, demonstrated the anti-aging properties of CM of epidermal progenitor cells derived from mesenchymal stem cells [13]. Moreover, CM derived from various stem cells have been used in anti-aging formulations and hair growth promoting cosmetic formulations [13][14][15].…”

“…Sohn et al, demonstrated the anti-aging properties of CM of epidermal progenitor cells derived from mesenchymal stem cells [13]. Moreover, CM derived from various stem cells have been used in anti-aging formulations and hair growth promoting cosmetic formulations [13][14][15]. Therefore, this study aimed to develop the BM-MSC derived CM based formulation and elucidate its UV-protective effects.…”

Comparative characterization of sun exposed and sun protected skin‐derived mesenchymal‐like stem cells in variegate porphyria and healthy individuals

“…Depending on the pathology, stem‐like skin‐derived cells help fibroblasts to either secrete wound healing related proteins or reduce ECM deposition 26 . Moreover, dermal fibroblasts have been used clinically for the treatment of various non‐healing wounds and genetic diseases 27‐29 . In our study, we investigated the effect of the secretome (secreted proteome/metabolome) produced by both stem‐like skin‐derived cells and fibroblasts to counter the damage of sunlight radiation.…”