Anti-Amyloidogenic and Cyclooxygenase Inhibitory Activity of Guettarda speciosa

Tan, Mario A.; Lagamayo, Mark Wilson D.; Alejandro, Grecebio Jonathan D.; An, Seong Soo A.

doi:10.3390/molecules24224112

Cited by 15 publications

(15 citation statements)

References 46 publications

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“…Of all documented Philippine medicinal plants, the top list of medicinal plants used for TCAM has been enumerated by [12]. Most of these Philippine medicinal plants have been evaluated to scientifically validate folkloric claims like the recent studies of [13][14][15][16][17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Quantitative ethnopharmacological documentation and molecular confirmation of medicinal plants used by the Manobo tribe of Agusan del Sur, Philippines

Dapar

Alejandro

Meve

et al. 2020

J Ethnobiology Ethnomedicine

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Background: The Philippines is renowned as one of the species-rich countries and culturally megadiverse in ethnicity around the globe. However, ethnopharmacological studies in the Philippines are still limited especially in the most numerous ethnic tribal populations in the southern part of the archipelago. This present study aims to document the traditional practices, medicinal plant use, and knowledge; to determine the relative importance, consensus, and the extent of all medicinal plants used; and to integrate molecular confirmation of uncertain species used by the Agusan Manobo in Mindanao, Philippines. Methods: Quantitative ethnopharmacological data were obtained using semi-structured interviews, group discussions, field observations, and guided field walks with a total of 335 key informants comprising of tribal chieftains, traditional healers, community elders, and Manobo members of the community with their medicinal plant knowledge. The use-report (UR), use categories (UC), use value (UV), cultural importance value (CIV), and use diversity (UD) were quantified and correlated. Other indices using fidelity level (FL), informant consensus factors (ICF), and Jaccard's similarity index (JI) were also calculated. The key informants' medicinal plant use knowledge and practices were statistically analyzed using descriptive and inferential statistics.

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Section: Introductionmentioning

confidence: 99%

Quantitative ethnopharmacological documentation and molecular confirmation of medicinal plants used by the Manobo tribe of Agusan del Sur, Philippines

Dapar

Alejandro

Meve

et al. 2020

J Ethnobiology Ethnomedicine

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“…As part of our continuing search for potential anti-AD agents exhibiting anti-amyloidogenic activity (Tan et al 2019(Tan et al , 2020, the crude extracts of Pandanus clementis Merr. (Pandanaceae) were observed to inhibit the Aβ aggregation using the ThT assay.…”

Section: Introductionmentioning

confidence: 99%

Neuroprotective effects on amyloid-beta induced cytotoxicity of Pandanus clementis Merr

Tan

Nonato

et al. 2021

3 Biotech

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The present study determined the neuroprotective potential of the alcoholic and aqueous extracts of Pandanus clementis Merr. (Pandanaceae) to protect the neuroblastoma SH-SY5Y cells against amyloid-beta 1-42 (Aβ) cytotoxicity. Inhibition of Aβ aggregation was determined by Thioflavin T (ThT) assay, and in vitro neuroprotective cell viability, intracellular reactive oxygen species (ROS), and mitochondrial membrane potential (MMP) were evaluated with human neuroblastoma SH-SY5Y cells insulted with Aβ. Chromatographic separation on the alcoholic extract yielded known phytosterols. Results showed that pretreatment of the SH-SY5Y cells with the P. clementis extracts increased cell viability and MMP, and decreased ROS, suggesting protective effects. Hence, P. clementis extract has promising neuroprotective therapeutic potential.

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“…The procedure for the ThT assay was previously reported [ 2 , 19 , 20 ]. Briefly, Aβ 1-42 (Aggresure™ (AnaSpec) Fremont, CA, USA) was dissolved in phosphate buffered saline (PBS) and incubated with or without the compounds or phenol red (positive control) at 37 °C for 24 h. ThT solution was added and incubated for 15 min.…”

Section: Methodsmentioning

confidence: 99%

“…Cell viability measurement was performed using the ATP luminescence assay as previously described [ 19 , 20 ]. SH-SY5Y cells were counted by Eve cell counter (NanoEntek, Inc., Seoul, Korea), and 2 × 10 4 cells/well density were sub-cultured in 96-well plate and incubated for 24 h. After incubation, cells were treated with the compounds for 24 h. The media were removed, cells were washed with PBS, fresh media were added, and incubated for another 30 min.…”

Section: Methodsmentioning

confidence: 99%

Diaportheone A Analogues Instigate a Neuroprotective Effect by Protecting Neuroblastoma SH-SY5Y Cells from Oxidative Stress

Tan

Захарова

2021

Biology

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Alzheimer’s disease (AD) remains an incurable neurodegenerative illness. Oxidative stress resulting in the formation of reactive oxygen species (ROS) and the abnormal deposition of amyloid-beta (Aβ) are the major pathological hallmarks associated with AD. In search for small molecules targeting multiple pathways of AD and of no known molecular targets, the neuroprotective effects of the synthetic chromones diaportheone A1 and diaportheone A2, analogues of the natural product diaportheone A, were investigated. Chromones are heterocyclic compounds bearing the benzoannelated γ-pyrone moiety and were regarded as an important class of organic molecules due to their diverse pharmacological activities. The influence of the compounds on the inhibition of Aβ aggregation was determined by Thioflavin T (ThT) assay, and the cell viability, ROS, and mitochondrial membrane potential were evaluated with human neuroblastoma SH-SY5Y cells. Results showed that both compounds inhibited the Aβ aggregation at 80.41% and 73.68% for diaportheone A1 and diaportheone A2, respectively. Increased cell viabilities were observed from the protection by both compounds using Aβ- or H2O2-induced SH-SY5Y cells. Both compounds also reduced the intracellular ROS level in Aβ- or H2O2-induced SH-SY5Y cells at 10 and 20 μM concentrations, and increased the mitochondrial membrane potentials in Aβ-induced SH-SY5Y cells at 20 μM concentration. Molecular docking experiments using the Aβ protein models 2MXU and 2BEG also indicated a good agreement with the experimental data. The results demonstrated for the first time the oxidative stress effects associated with the chromones diaportheone A1 and diaportheone A2 as potential neuroprotective therapeutic agents against AD.

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Anti-Amyloidogenic and Cyclooxygenase Inhibitory Activity of Guettarda speciosa

Cited by 15 publications

References 46 publications

Quantitative ethnopharmacological documentation and molecular confirmation of medicinal plants used by the Manobo tribe of Agusan del Sur, Philippines

Quantitative ethnopharmacological documentation and molecular confirmation of medicinal plants used by the Manobo tribe of Agusan del Sur, Philippines

Neuroprotective effects on amyloid-beta induced cytotoxicity of Pandanus clementis Merr

Diaportheone A Analogues Instigate a Neuroprotective Effect by Protecting Neuroblastoma SH-SY5Y Cells from Oxidative Stress

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