Anti‐AnWj causing acute hemolytic transfusion reactions in a patient with aplastic anemia

Abstract

A case of acute hemolytic transfusion reaction caused by anti-AnWj is reported in a patient with aplastic anemia requiring allogeneic stem cell transplantation. This is the second documented case of anti-AnWj to cause a hemolytic transfusion reaction. The case demonstrates the complexity of managing patients with rare antibodies and the importance of international collaboration in the management of these difficult cases.

Evaluation of the functional properties of cryopreserved buffy coat–derived monocytes for monocyte monolayer assay

“…Branch, unpublished results), and anti‐Jr a alloantibodies showed a positive MMA reactivity (PI > 5). In addition to these findings, a number of other case reports support this assessment . RBCs expressing Sc2 antigens are extremely rare, as are the antibodies against them .…”

“…It is for this reason that we performed MMA using cryopreserved Sc2‐positive RBCs. In contrast, AnWj and Jr a RBC antigens are high‐frequency antigens, which make antibodies against these antigens very rare . These alloantibodies are of IgG type and may be formed during pregnancy or after transfusion .…”

“…Scianna‐2 (Sc2)‐positive RBCs were cryopreserved as pellets in a solution of dextrose/sucrose/NaCl and K 2 EDTA/NaOH and CPDA‐1 (CBS Diagnostic Services). Two RBC units were randomly selected and tested against anti‐AnWj or anti‐Jr a , as these antigens occur at high frequencies in each population . RBCs were sensitized by addition of equal volume of anti‐D (human polyclonal IgG, Gamma Biologicals/Immucor, a gift from M. Moulds) or 4 vol of patient serum containing either anti‐Sc2, anti‐AnWj, or anti‐Jr a alloantibodies, confirmed by the CBS National Reference Laboratory, followed by 1 hour of incubation (37°C, 5% CO 2 ).…”

Changes in Erythrocyte Surface Marker Cd44 During Hypothermic and Low Temperature Storage

“…Останній являє собою молекулу адгезії, яка опосередковує зв'язок клітин з гіалуроновою кислотою та іншими компонен-тами позаклітинного матриксу [3,4]. В ери-троцитах СD44 представлений стандартною ізоформою (СD44s), яка також називається гемопоетичною (СD44H), та презентує анти-гени двох різних систем груп крові: AnWj і In a/b [5,6]. У нормі еритроцити людини не виявляють адгезивної активності, але залу-чені в розвиток патофізіологічних процесів, пов'язаних з вазооклюзивними станами при серпоподібноклітинній анемії, спадковому сфероцитозі та інших захворюваннях [7].…”

Effect of substances with cryoprotective properties on surface marker CD44 in human erythrocytes

“…Prevalent antigens AnWj are located in the proximal region of the extracellular domain of CD44 [5]. Antigens In(a/b) were identified within the hyaluronic acid binding domain and are located in the distal region of CD 44 molecule.…”