Anti-arthritic Effects of Platelets Rich Plasma and Hyaluronic Acid on Adjuvant-induced Arthritis in Rats

Aniss, Nadia Noble-Daoud; Zaazaa, Asmaa M; Saleh, Mohamed Rabie Abdall

doi:10.3923/ijp.2020.33.46

Cited by 9 publications

(8 citation statements)

References 54 publications

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“…It was evidenced effective in diminishing hyperplasia of synovial membrane, cartilage destruction, and bone erosion degree. These results are in agreement with the previous study [ 23 ].…”

Section: Discussionsupporting

confidence: 94%

“…In the current study, PRP produced a significant decrease in the elevated values of the right ankle anteroposterior and transverse diameters when compared to MIA animals after the 4 th and 3 rd weeks, respectively. In parallel with this study, Aniss et al (2020) stated that the treatment of rats with PRP for six weeks in CFA-induced arthritis results in the decline of paw swelling [ 23 ].…”

Section: Discussionmentioning

confidence: 57%

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Platelet-Rich Plasma Ameliorates Monosodium Iodoacetate-Induced Ankle Osteoarthritis in the Rat Model via Suppression of Inflammation and Oxidative Stress

Ragab

Halfaya

Ahmed

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Until now, there is no treatment that cause complete cure of the chronic inflammatory and degenerative disease, osteoarthritis (OA). Moreover, the underlying mechanisms of OA development and progress are not fully elucidated, and the present pharmacological treatment alternatives are restricted and associated with adverse side effects. Thus, the present study was conducted to evaluate the role of platelet-rich plasma (PRP) in the remedy of OA in the rat model in terms of inflammation, ankle histopathological alterations, and oxidative stress. OA was induced in male Wistar rats by injection of MIA (2 mg)/50 µL isotonic saline in the right ankle joint for two successive days in each rat. After the 2nd MIA injection, the osteoarthritic rats were allocated into two groups such as the MIA group (group 2) and MIA + PRP group (group 3). The MIA + PRP group was treated with PRP (50 µL) by injection into the ankle joint of the right hind limb of each rat at days 14, 21, and 28 after the 2nd injection of MIA. The same equivalent volume of saline, as a substitute of PRP, was injected into the ankle joint of each rat of the normal control group (group 1) and MIA group (group 2) at the same tested periods. Swelling of joint, bodyweight, total leucocytes count (TLC), and morphological as well as histological changes of ankle joints were evaluated. Serum lipid peroxides (LPO), glutathione (GSH), and glutathione S-transferase (GST) levels were examined as biomarkers of oxidative stress. Serum tumor necrosis factor-α (TNF-α), interleukin-17 (IL-17), and interleukin-4 (IL-4) were investigated by ELISA as biomarkers of inflammation. In addition, magnetic resonance imaging (MRI) was carried out to investigate the soft tissues in joints. The obtained results revealed that PRP reduced LPO and increased GSH and GST levels in osteoarthritic rats. Also, PRP significantly diminished serum TNF-α and IL-17 levels, while it increased IL-4 serum levels in rats with MIA-induced OA. Morphological observations, histological analysis, and MRI revealed a gradual diminishing in joint inflammation and destruction of cartilage in PRP-injected osteoarthritic rats. Based on these results, it can be suggested that PRP has antiarthritic potential in MIA-induced OA, which may be mediated via suppression of inflammation and oxidative stress.

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“…It was evidenced effective in diminishing hyperplasia of synovial membrane, cartilage destruction, and bone erosion degree. These results are in agreement with the previous study [ 23 ].…”

Section: Discussionsupporting

confidence: 94%

Section: Discussionmentioning

confidence: 57%

Platelet-Rich Plasma Ameliorates Monosodium Iodoacetate-Induced Ankle Osteoarthritis in the Rat Model via Suppression of Inflammation and Oxidative Stress

Ragab

Halfaya

Ahmed

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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“…Microscopical examination of the stifle joint tissues obtained from normal control rats (Group I) revealed normal histological appearance of synovium and stifle joint capsule with an absence of inflammation; this joint preserved also normal architecture with intact articular cartilage and a well-defined joint space as shown in Figure (1). Histopathological examination of the stifle joint obtained from the protected group with GSPE (Group V) revealed edema of the synovium and formation of a few papillary villar projections lined by hypertrophied and hyperplastic synovial cells without signs of inflammation as demonstrated in Figure (5). Macrophages and lymphocytes mainly infiltrated the fibrous joint capsule.…”

Section: Resultsmentioning

confidence: 90%

“…Li et al,(2018), recorded a marked increase in the level of tumor necrosis factor alpha (TNFα), nuclear factor kappa B (NF-κB), interleukin-1β (IL-1β) and nuclear factor erythroid 2-related factor 2 (Nrf2) in the arthritic rats. Also, a significant amplification in matrix metalloproteinase-3 (MMP-3) gene expression was detected by Aniss et al, (2020). Currently, 80% of the world population utilizes the plantbased medications for successful RA pain relief (Sahoo et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Beneficial anti-inflammatory and anti-arthritic activities of Grape seed proanthocyanidin extract in Freund's Complete Adjuvant-induced Rheumatoid arthritis in rats

Elbahlol

Hussein

Abdelal

et al. 2021

Benha Veterinary Medical Journal

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Rheumatoid arthritis (RA) is a systemic autoimmune inflammatory disorder with massive suffering and permanent joint destruction. The Grape seed proanthocyanidin extract (GSPE) beneficial effects were evaluated in adjuvant-induced arthritis (AIA). RA was induced by 0.1 mL Freund's complete adjuvant (CFA) that was injected once into the rat's right hind leg footpads. Thirty male rats were equally divided into 5 groups. Group I (Normal control): Rats received no drugs, Group II (CFA-induced arthritis for treatment): Rats injected with 0.1 ml CFA on the 1 st day and sacrificed after 29 days of CFA injection, Group III (CFA + GSPE treated): After 14 days of CFA injection, rats treated with GSPE (100 mg . kg -1 b wt./day) for 15 sequential days. Group IV (CFA-induced arthritis for protection): Rats injected with 0.1 ml CFA on 15 th day of the experiment and sacrificed 15 days later after arthritis induction. Group V (CFA + GSPE protected): Rats pretreated with GSPE (100 mg . kg -1 b wt./day) one day before CFA injection and 14 days later after arthritis induction. A significant upregulation in IL-1β, MMP-3 and LOX-1 gene expressions were observed in the arthritic rats' synovial membrane. However, significant down-regulations in all previous parameters were observed in GSPE treated groups. Also, GSPE significantly activated Nrf2 gene in the liver with marked up-regulation of Lactoferrin (LF) gene in arthritic rats' synovial membrane. Interestingly, improvement of histopathological alterations in synovium and stifle joint capsule of arthritic rats supported the anti-arthritic effects of GSPE. Conclusively, GSPE ameliorates adjuvant induced arthritis via its anti-arthritic and joint protecting properties.

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“…[32], Woodell-May et al [97] and Aniss et al [98] support the use of PRP as a therapeutic intervention to promote joint repair in OA. These play an important role in chondrocyte terminal differentiation in murine embryos [99].…”

Section: The Improvement On Gait Dysfunction Induced By Lp-prp On Mia...mentioning

confidence: 99%

The implications of Brain-derived neurotrophic factor in the biological activities of platelet-rich plasma.

Malange,

de Souza,

Lemes

et al. 2024

Preprint

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Platelet-rich plasma (PRP) is a biological blood-derived therapeutic that releases several proteins in injured tissue, regulating the inflammatory process and triggering tissue anabolism. Outside of the central nervous system (CNS), these platelets represent the major source of brain-derived neurotrophic factor (BDNF). The associated anti-inflammatory, antiapoptotic, and antioxidant properties of this neurotrophin could have a potential applicability applied in the pathophysiology of osteoarthritis (OA). In osteoarthritic joints, it is suggested that synovial cells release BDNF, being found higher concentrations of this protein in the patient’s synovial fluid. Thus, considering platelets as an important therapeutic source of BDNF and the potential role of this neurotrophin in regulate joint homeostasis and OA outcomes, this study aimed to evaluate the contribution of BDNF in PRP repair activities on an experimental model of osteoarthritis (EOA) induced by monoiodine acetate (MIA) insult. Initially, the characterization of the PRP was performed, obtaining a leukocyte-poor-platelet-rich plasma (LP-PRP). After, in vitro analysis indicated that platelets activated by Ca2+ release BDNF, and suppression of M1 macrophage polarization induced by LP-PRP is dependent on BDNF-TrkB receptor. The behavioral and ex-vivo analyses in EOA animals showed that functional recovery in gait parameters and mitigation on tissue damage induced by LP-PRP, such as downregulation of inflammatory cytokines (TNF-α), reduction of joint pain and tissue damage (OARSI score and neuronal injury through ATF-3 [+] cells), depends on Trk-B receptor. Thus, the results indicate that BDNF and Trk-B receptor appear to be essential in the mechanisms of joint repair induced by LP-PRP with implications on joint functional recovery as well. These findings contribute to clarifying the role of BDNF in joint homeostasis, repurposing its restricted therapeutic properties in the CNS to repair functions in non-neuronal tissues that could be an option for chronic pain management in osteoarthritis (OA).

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Anti-arthritic Effects of Platelets Rich Plasma and Hyaluronic Acid on Adjuvant-induced Arthritis in Rats

Cited by 9 publications

References 54 publications

Platelet-Rich Plasma Ameliorates Monosodium Iodoacetate-Induced Ankle Osteoarthritis in the Rat Model via Suppression of Inflammation and Oxidative Stress

Platelet-Rich Plasma Ameliorates Monosodium Iodoacetate-Induced Ankle Osteoarthritis in the Rat Model via Suppression of Inflammation and Oxidative Stress

Beneficial anti-inflammatory and anti-arthritic activities of Grape seed proanthocyanidin extract in Freund's Complete Adjuvant-induced Rheumatoid arthritis in rats

The implications of Brain-derived neurotrophic factor in the biological activities of platelet-rich plasma.

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