Anti-cancer activities of allyl isothiocyanate and its conjugated silicon quantum dots

Liu, Peng; Behray, Mehrnaz; Wang, Qi; Wang, Wei; Zhou, Zhigang; Chao, Yimin; Bao, Yongping

doi:10.1038/s41598-018-19353-7

Cited by 55 publications

(37 citation statements)

References 54 publications

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“…Nrf2 knockdown was achieved by siRNA transfection. The siRNA knockdown efficiency of Nrf2 was measured using Western blot analysis as previous described 45 .…”

Section: Effect Of Sfn On Intracellular Gsh Levels and Nrf2 Pathwaymentioning

confidence: 99%

Antioxidant effects of sulforaphane in human HepG2 cells and immortalised hepatocytes

Liu

Wang

Tang

et al. 2019

Food and Chemical Toxicology

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Sulforaphane (SFN) has shown anti-cancer effects in cellular and animal studies but its effectiveness has been limited in human studies. Here, the effects of SFN were measured in both human hepatocyte (HHL5) and hepatoma (HepG2) cells. Results showed that SFN inhibited cell viability and induced DNA strand breaks at high doses (≥ 20 µM). It also activated the nuclear factor (erythroidderived 2)-like 2 (Nrf2), and increased intracellular glutathione (GSH) levels at 24 hours. Pretreatment with a low dose SFN (≤5 µM) protected against hydrogen peroxide (H 2 O 2)-induced cell damage. High doses of SFN were more toxic towards HHL5 compared to HepG2 cells; the difference is likely due to the disparity in the responses of Nrf2-driven enzymes and-GSH levels between the two cell lines. In addition, HepG2 cells hijacked the cytoprotective effect of SFN over a wider dose range (1.25-20 µM) compared to HHL5. Manipulation of levels of GSH and Nrf2 in HepG2 cells confirmed that both molecules mediate the protective effects of SFN against H 2 O 2. The non-specific nature of SFN in the regulation of cell death and survival could present undesirable risks, i.e. be more toxic to normal cells, and cause chemo-resistance in tumor cells. These issues should be addressed in the context for cancer prevention and treatment before large scale clinical trials are undertaken.

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“…Nrf2 knockdown was achieved by siRNA transfection. The siRNA knockdown efficiency of Nrf2 was measured using Western blot analysis as previous described 45 .…”

Section: Effect Of Sfn On Intracellular Gsh Levels and Nrf2 Pathwaymentioning

confidence: 99%

Antioxidant effects of sulforaphane in human HepG2 cells and immortalised hepatocytes

Liu

Wang

Tang

et al. 2019

Food and Chemical Toxicology

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“…Medical plants and their bioactive components are widely used worldwide for the management of DM and complications due to their nontoxic nature, obtainability, and affordability. One of them, Allyl isothiocyanate (AITC), a natural compound found in many vegetables, has been reported to possess antiobesity, antimicrobial, anticancer and analgesic and anti‐inflammatory properties. AITC inhibits hepatic gluconeogenesis and ameliorates insulin resistance .…”

Section: Introductionmentioning

confidence: 99%

Effects of allyl isothiocyanate on insulin resistance, oxidative stress status, and transcription factors in high‐fat diet/streptozotocin‐induced type 2 diabetes mellitus in rats

Şahin

Orhan

Erten

et al. 2019

J Biochem & Molecular Tox

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Allyl isothiocyanate (AITC), a dietary phytochemical found in some cruciferous vegetables, is commonly used as an antimicrobial, anticancer, and antioxidant agent.In the present study, we investigated the effects of AITC on insulin resistance and transcription factors in a diabetic rat model. A total of 42 Wistar rats were divided into six groups and orally treated for 12 weeks as follows: (i) control; (ii) AITC (100 mg/kg body weight [BW]); (iii) high fat diet (HFD); (iv) HFD + AITC (100 mg/kg BW); (v) HFD + streptozotocin (STZ, 40 mg/kg BW); and (vi) HFD + STZ + AITC.Administration of AITC reduced blood glucose, total cholesterol, triglycerides, and creatinine levels, but increased (P < 0.001) total antioxidant capacity. In AITC-treated rats, the glucose transporter-2, peroxisome proliferator-activated receptor gamma, p-insulin receptor substrate-1, and nuclear factor erythroid-derived 2 in the liver and kidney were increased while nuclear factor-kappa B was downregulated (P < 0.05). In conclusion, AITC possesses antidiabetic, antioxidant, and anti-inflammatory activities in HFD/STZ-induced T2DM in rats. These findings may further justify the importance of AITC in phytomedicine. K E Y W O R D Sallyl isothiocyanate, diabetes, insulin resistance, oxidative stress, streptozotocin

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“…Then cells were treated with 10 µM SFN or its metabolites for 4 or 18 h with DMSO (0.1%) as control. The extraction was performed as described previously [ 32 ].…”

Section: Experimental Methodsmentioning

confidence: 99%

Chemopreventive Activities of Sulforaphane and Its Metabolites in Human Hepatoma HepG2 Cells

et al. 2018

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Sulforaphane (SFN) exhibits chemopreventive effects through various mechanisms. However, few studies have focused on the bioactivities of its metabolites. Here, three metabolites derived from SFN were studied, known as sulforaphane glutathione, sulforaphane cysteine and sulforaphane-N-acetylcysteine. Their effects on cell viability, DNA damage, tumorigenicity, cell migration and adhesion were measured in human hepatoma HepG2 cells, and their anti-angiogenetic effects were determined in a 3D co-culture model of human umbilical vein endothelial cells (HUVECs) and pericytes. Results indicated that these metabolites at high doses decreased cancer cell viability, induced DNA damage and inhibited motility, and impaired endothelial cell migration and tube formation. Additionally, pre-treatment with low doses of SFN metabolites protected against H2O2 challenge. The activation of the nuclear factor E2-related factor 2 (Nrf2)-antioxidant response element (ARE) pathway and the induction of intracellular glutathione (GSH) played an important role in the cytoprotective effects of SFN metabolites. In conclusion, SFN metabolites exhibited similar cytotoxic and cytoprotective effects to SFN, which proves the necessity to study the mechanisms of action of not only SFN but also of its metabolites. Based on the different tissue distribution profiles of these metabolites, the most relevant chemical forms can be selected for targeted chemoprevention.

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Anti-cancer activities of allyl isothiocyanate and its conjugated silicon quantum dots

Cited by 55 publications

References 54 publications

Antioxidant effects of sulforaphane in human HepG2 cells and immortalised hepatocytes

Antioxidant effects of sulforaphane in human HepG2 cells and immortalised hepatocytes

Effects of allyl isothiocyanate on insulin resistance, oxidative stress status, and transcription factors in high‐fat diet/streptozotocin‐induced type 2 diabetes mellitus in rats

Chemopreventive Activities of Sulforaphane and Its Metabolites in Human Hepatoma HepG2 Cells

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