Anti-Endothelial Cell Antibodies and Central Nervous System Involvement in Behçet's Disease

Souza, R.C.; Lage, Luis Alberto de Padua Covas; Goldesntein-Schainberg, Cláudia; Macedo, André Regis; Carrasco, Solange; Gonçalves, Célio Roberto

doi:10.1590/s1807-59322007000600005

Cited by 14 publications

(5 citation statements)

References 24 publications

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“…In contrast with ANCA, AECA is detected widely in various types of systemic vasculitis [10][11][12][13][14][15][16][17][18]. The presence of AECA is known to be correlated with the activity of vasculitis [12,15,19].…”

Section: Introductionmentioning

confidence: 99%

“…The presence of AECA is known to be correlated with the activity of vasculitis [12,15,19]. Further, AECA is reported to be associated with particular clinical manifestations; for example, acute thrombotic events, retinal vasculitis and involvement of the central nervous system in BD [16,17], and vascular lesions, nephropathy and production of anti‐cardiolipin antibodies in SLE [15,20]. AECA has been reported to be involved in endothelial cell damage and vascular injury by its binding to endothelial cell surface antigens [10,20].…”

Section: Introductionmentioning

confidence: 99%

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Peroxiredoxin 2 is a novel autoantigen for anti-endothelial cell antibodies in systemic vasculitis

Karasawa

Kurokawa

Yudoh

et al. 2010

Clinical and Experimental Immunology

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SummaryAnti-endothelial cell antibodies (AECA) have been frequently detected in systemic vasculitis, which affects blood vessels of various sizes. To understand the pathogenic roles of AECA in systemic vasculitis, we attempted to identify target antigens for AECA comprehensively by a proteomic approach. Proteins extracted from human umbilical vein endothelial cells (HUVEC) were separated by two-dimensional electrophoresis, and Western blotting was subsequently conducted using sera from patients with systemic vasculitis. As a result, 53 autoantigenic protein spots for AECA were detected, nine of which were identified by mass spectrometry. One of the identified proteins was peroxiredoxin 2 (Prx2), an anti-oxidant enzyme. Frequency of anti-Prx2 autoantibodies, measured by enzyme-linked immunosorbent assay (ELISA), was significantly higher in systemic vasculitis (60%) compared to those in collagen diseases without clinical vasculitis (7%, P < 0·01) and healthy individuals (0%, P < 0·01). Further, the titres changed in parallel with the disease activity during time-courses. The presence of anti-Prx2 autoantibodies correlated significantly with elevation of serum d-dimers and thrombinantithrombin complex (P < 0·05). Immunocytochemical analysis revealed that live endothelial cells expressed Prx2 on their surface. Interestingly, stimulation of HUVEC with rabbit anti-Prx2 antibodies increased secretion of interleukin (IL)-6, IL-1b, IL-1ra, growth regulated oncogene (GRO)-a, granulocyte colony-stimulating factor (G-CSF), granulocyte macrophage colonystimulating factor (GM-CSF), IL-8 and monocyte chemoattractant protein (MCP)-1 more than twofold compared to that of with rabbit immunoglobulin (Ig)G. Taken together, our data suggest that anti-Prx2 autoantibodies would be a useful marker for systemic vasculitis and would be involved in the inflammatory processes of systemic vasculitis.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Peroxiredoxin 2 is a novel autoantigen for anti-endothelial cell antibodies in systemic vasculitis

Karasawa

Kurokawa

Yudoh

et al. 2010

Clinical and Experimental Immunology

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“…IgG-AECA and central nervous system involvement in ABD was reported (Dinc et al, 2003;Souza et al, 2007;Zeng et al, 2004). Alpha-enolase represents a target antigen of IgM AECA, reported to be detectable in approximately half of ABD patients, especially in those with overt vascular involvement; however, its specificity is not satisfying (Lee JH et al, 2009).…”

Section: B Cell Abnormalities and Autoantibodies In Abdmentioning

confidence: 96%

Advances in the Diagnosis and Treatment of Vasculitis

Amezcua-Guerra¹

2011

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His work has been seminal to identify and characterize the immune mechanisms that underlie erosive arthritis in systemic lupus erythematosus, especially those related to the abnormal behavior of citrulline and C-reactive protein. He has also been involved in the study of kidney damage in Takayasu's arteritis as well as in the existence of subclinical tissue damage in patients with asymptomatic hyperuricemia. Dr. Amezcua-Guerra teaches immunology to postgraduate students in Rheumatology at the Universidad Nacional Autónoma de México and to undergraduate students at the

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“…Despite their inspecificity, AECA were shown to mediate enhanced polymorphonuclear adhesion to activated endothelial cells, thus promoting tissue injury during systemic inflammation. An association between IgG-AECA and central nervous system involvement in ABD was reported (Dinc et al, 2003;Souza et al, 2007;Zeng et al, 2004). Alpha-enolase represents a target antigen of I g M A E C A , r e p o r t e d t o b e d e t e c t a b l e i n approximately half of ABD patients, especially in those with overt vascular involvement; however, its specificity is not satisfying (Lee JH et al, 2009).…”

Section: B Cell Abnormalities and Autoantibodies In Abdmentioning

confidence: 97%