Anti-fouling poly(2-hydoxyethyl methacrylate) surface coatings with specific bacteria recognition capabilities

Mrabet, Béchir; Nguyen, Minh Ngoc; Majbri, Aymen; Mahouche, Samia; Turmine, Mireille; Bakhrouf, Amina; Chehimi, Mohamed M.

doi:10.1016/j.susc.2009.05.020

Cited by 74 publications

(47 citation statements)

References 39 publications

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“…Typical functional groups responsible for the fouling resistance of hydrophilic antifouling polymers are ether, hydroxyl, amide, peptoid, β‐peptoid, and open‐ring oxazoline (Figure ). Antifouling polymers of this type include ethylene glycol‐based polymers, poly(2‐hydroxyethyl methacrylate) (pHEMA), poly(hydroxypropyl methacrylate) (pHPMA), polysaccharides such as dextran and cellulose, polyacrylamide, polypeptoids, poly(β‐peptoid)s, and polyalkyloxazoline . The antifouling functional groups could be a part of the polymer backbone or the side chain.…”

Section: Materials Chemistry (Type Of Materials)mentioning

confidence: 99%

Recent development of antifouling polymers: structure, evaluation, and biomedical applications in nano/micro‐structures

Liu

2014

WIREs Nanomed Nanobiotechnol

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Antifouling polymers have been proven to be vital to many biomedical applications such as medical implants, drug delivery, and biosensing. This review covers the major development of antifouling polymers in the last 2 decades, including the material chemistry, structural factors important to antifouling properties, and how to challenge or evaluate the antifouling performances. We then discuss the applications of antifouling polymers in nano/micro-biomedical applications in the form of nanoparticles, thin coatings for medical devices (e.g., artificial joint, catheter, wound dressing), and nano/microscale fibers.

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Section: Materials Chemistry (Type Of Materials)mentioning

confidence: 99%

Recent development of antifouling polymers: structure, evaluation, and biomedical applications in nano/micro‐structures

Liu

2014

WIREs Nanomed Nanobiotechnol

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“…It is well known that organic polymer coatings permit to tune the surface properties of materials such as wetting, [1] adhesion, [2] chemical sensing; [3,4] response to chemical, temperature, pH, ionic strength, light, and mechanical stimuli; [5] cell adhesion, [6] resistance to protein adsorption [7] and bacterial fouling, [8] to name but a few.…”

Section: Introductionmentioning

confidence: 99%

“…[14] The polymer grafts were prepared by atom transfer radical polymerization (ATRP) initiated by diazoniummodified substrates instead of the traditional methods employing grafted initiators from self assembled monolayers of thiols on gold [7] or silanes on glass. [8] Towards this end, ATRP initiators were electrografted to gold via the electrochemical reduction of the noncommercial diazonium salt BF 4 − , + N 2 -C 6 H 4 -CH(CH 3 )Br to provide Au-C 6 H 4 -CH(CH 3 )Br plates (Au-Br) serving as macroinitiators for the ATRP of oligoethylene glycol methacrylate. The goldgrafted poly(oligoethylene glycol methacrylate) (Au-POEGMA) plates and reference materials were characterized by XPS, polarization modulation-infrared reflection-absorption spectroscopy (PM-IRRAS) and contact angle measurements.…”

Section: Introductionmentioning

confidence: 99%

Controlled adhesion of Salmonella Typhimurium to poly(oligoethylene glycol methacrylate) grafts

Mrabet

Mejbri

Mahouche

et al. 2011

Surface & Interface Analysis

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International audiencePoly(oligoethylene glycol methacrylate), POEGMA, brushes were prepared by surface-initiated atom transfer radical polymerization (SI-ATRP) on gold-coated silicon wafers. Prior to ATRP, the substrates were grafted by brominated aryl initiators via the electrochemical reduction of a noncommercial parent diazonium salt of the formula BF4-, N-+(2)-C6H4-CH(CH3)Br. The diazonium-modified gold plates (Au-Br) served as macroinitiators for ATRP of OEGMA which resulted in hydrophilic surfaces (Au-POEGMA) that could be used for two distinct objectives: (i) resistance to fouling by Salmonella Typhimurium; (ii) specific recognition of the same bacteria provided that the POEGMA grafts are activated by anti-Salmonella. The Au-POEGMA plates were characterized by XPS, polarization modulation-infrared reflection-absorption spectroscopy (PM-IRRAS) and contact angle measurements. Both Beer-Lambert equation and Tougaard's QUASES software indicated a POEGMA thickness that exceeds the critical similar to 10 nm value necessary for obtaining a hydrophilic polymer with effective resistance to cell adhesion. The Au-POEGMA slides were further activated by trichlorotriazine (TCT) in order to covalently bind anti-Salmonella antibodies (AS). The antibody-modified Au-POEGMA specimens were found to specifically attach Salmonella Typhimurium bacteria. This work is another example of the diazonium salt/ATRP process to provide biomedical polymer surfaces

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“…The P(HEMA‐co‐GMA) brushes (PHG) generated via SI‐AGET‐ATRP were chosen as a substrate for covalent immobilization of peptides. PHEMA as a hydrophilic polymer has been widely used to prevent non‐specific adsorption of proteins and cells . Meantime, each GMA unit can provide an epoxy group to react with the peptides by the “thiol‐epoxy” click reaction .…”

Section: Resultsmentioning

confidence: 99%

Selective Adhesion and Directional Migration of Endothelial Cells Guided by Cys‐Ala‐Gly Peptide Density Gradient on Antifouling Polymer Brushes

Gao

2019

Macromolecular Bioscience

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Selective adhesion and directional migration of endothelial cells (ECs) on biomaterials is critical to realize the rapid endothelialization. In this study, a Cys‐Ala‐Gly (CAG) peptide density gradient is generated on homogeneous cell‐resisting poly(2‐hydroxyethyl methacrylate‐co‐glycidyl methacrylate) brushes by immersing the brushes in a complementary gradient solution of CAG and competitive mercapto‐terminated methoxyl poly(ethylene glycol). The adhesion and spreading of smooth muscle cells (SMCs) is impaired effectively on the gradient surface. About six folds of adherent ECs over SMCs are achieved at the position (10 mm) of highest CAG density on the gradient surface in a co‐culture condition. Due to the gradient cues, ECs migrate fastest with the best directionality of 86.7% at the middle of the gradient, leading to the maximum net displacement as well.

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Anti-fouling poly(2-hydoxyethyl methacrylate) surface coatings with specific bacteria recognition capabilities

Cited by 74 publications

References 39 publications

Recent development of antifouling polymers: structure, evaluation, and biomedical applications in nano/micro‐structures

Recent development of antifouling polymers: structure, evaluation, and biomedical applications in nano/micro‐structures

Controlled adhesion of Salmonella Typhimurium to poly(oligoethylene glycol methacrylate) grafts

Selective Adhesion and Directional Migration of Endothelial Cells Guided by Cys‐Ala‐Gly Peptide Density Gradient on Antifouling Polymer Brushes

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