Anti-fungal activity of phenolic sweet orange peel extract for controlling fungi responsible for post-harvest fruit decay

Hernández, Alejandro; Ruíz-Moyano, Santiago; Galván, Ana Isabel; Merchán, Almudena V.; Nevado, Francisco Pérez; Aranda, Emilio; Serradilla, Manuel Joaquín; Córdoba, Maria de Guı́a; Martı́n, Alberto

doi:10.1016/j.funbio.2020.05.005

Cited by 56 publications

(21 citation statements)

References 48 publications

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“…The citrus processing industry produces an amount of waste that reaches more than 40 million tons worldwide, representing approximately 50% of the weight of the initial raw material. Commercial exploitation of the by‐products of citrus processing would represent an economic benefit by adding value to the citrus wastes (Hernández et al, 2021). Flavonoids are bioactive compounds with preeminent functions in plant metabolism, such as pigmentation and antioxidant activity.…”

Section: Introductionmentioning

confidence: 99%

Citrus flavonoids against Fusarium verticillioides in post-harvest maize: Minimization of fumonisins and alteration of fungal ultrastructure

Pok

Londoño

Vicente

et al. 2022

Journal of Applied Microbiology

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Aims To minimize fumonisins (FBs) accumulation by Fusarium verticillioides in post‐harvest maize, using flavonoids obtained from citrus residues: naringin (NAR), neohesperidin (NEO), quercetin (QUER), and its mixtures. Methods and Results Response surface methodology with Box‐Behnken design was applied in maize at 0.98 and 0.95 aw. The optimal mixture found, composed of 0.40 mmol kg−1 NAR, 0.16 mmol kg−1 NEO and 0.37 mmol kg−1 QUER, reduced the accumulation of FBs B1, B2, and B3 by 88 ± 6%, 90 ± 6% and 85 ± 5%, respectively, when applied to maize at 0.98 aw. The mentioned mixture led to a 54 ± 9% reduction of fumonisin B1 accumulation in maize adjusted to 0.95 aw. These flavonoids applied individually and as a mixture, affected the structure of both the cell wall and the cytoplasm of F. verticillioides. The cell wall lost rigidity and the cells appeared highly deformed, with ruptured plasmalemma and disrupted endomembranes. Conclusions It was possible to diminish the accumulation of FBs in maize by a highly toxigenic Fusarium strain, producing severe damage to its ultrastructure. Significance and Impact of Study The results indicate the possible use of flavonoids from citrus industry residues as natural and environmentally friendly antifungal agents to restrain the accumulation of FBs in stored maize.

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Section: Introductionmentioning

confidence: 99%

Citrus flavonoids against Fusarium verticillioides in post-harvest maize: Minimization of fumonisins and alteration of fungal ultrastructure

Pok

Londoño

Vicente

et al. 2022

Journal of Applied Microbiology

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“…With reference to composition in phenolic compounds, analyses evidenced the presence, in all tested substances, of molecules whose antimicrobial activity is supported by a wide range of literature [2,[36][37][38][39][40][41][42][43][44]. Some of these compounds have been also applied as eco-friendly alternatives to synthetic fungicides [1,45].…”

Section: Discussionmentioning

confidence: 99%

“…Vanillin (4-hydroxy-3-methoxybenzaldehyde) is considered one of the most important additives used in the food industry; it is characterized by effective inhibitory activity toward a wide range of microorganisms, thus causing a delay in the growth of yeasts and fungi [36,40]. The p-coumaric acid (4-hydroxycinnamic acid), which, in "Nitric-extract", had the highest concentration, is the main phenolic acid contained in the peel of sweet oranges [44], and is well known for its efficacy in negatively affecting the growth of post-harvest pathogens, such as Monilinia fructicola, Botrytis cinerea and Alternaria alternata [2]. Interestingly, "Nitricextract" also reported the highest concentration of catechol (1-2-dihydroxybenzene) and the exclusive presence of dihydroferulic (3-(4-hydroxy-3-methoxyphenyl) propionic acid) and ellagic acids.…”

Section: Discussionmentioning

confidence: 99%

“…In a horizontal direction, for each pathogen, values with different bold letters are statistically different according to Tukey's honestly significant difference (HSD) test (p ≤ 0.05) 2. In the vertical direction, for the concentrations 25% Nitric-extract, 50% Nitric-extract, 75% Nitric-extract, 100% Nitric-extract, values with different letters (in italic and within brackets) are statistically different according to Tukey's honestly significant difference (HSD) test (p ≤ 0.05).…”

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confidence: 99%

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Inhibitory Activity of Shrimp Waste Extracts on Fungal and Oomycete Plant Pathogens

Boumlasy

Spada

Tuccitto

et al. 2021

Plants

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(1) Background: This study was aimed at determining the in vitro inhibitory effect of new natural substances obtained by minimal processing from shrimp wastes on fungi and oomycetes in the genera Alternaria, Colletotrichum, Fusarium, Penicillium, Plenodomus and Phytophthora; the effectiveness of the substance with the highest in vitro activity in preventing citrus and apple fruit rot incited by P. digitatum and P. expansum, respectively, was also evaluated. (2) Methods: The four tested substances, water-extract, EtOAc-extract, MetOH-extract and nitric-extract, were analyzed by HPLC-ESI-MS-TOF; in vitro preliminary tests were carried out to determine the minimal inhibitory/fungicidal concentrations (MIC and MFC, respectively) of the raw dry powder, EtOAc-extract, MetOH-extract and nitric-extract for each pathogen. (3) Results: in the agar-diffusion-assay, nitric-extract showed an inhibitory effect on all pathogens, at all concentrations tested (100, 75, 50 and 25%); the maximum activity was on Plenodomus tracheiphilus, C. gloeosporioides and Ph. nicotianae; the diameters of inhibition halos were directly proportional to the extract concentration; values of MIC and MFC of this extract for all pathogens ranged from 2 to 3.5%; the highest concentrations (50 to 100%) tested in vivo were effective in preventing citrus and apple fruit molds. (4) Conclusions: This study contributes to the search for natural and ecofriendly substances for the control of pre- and post-harvest plant pathogens.

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“…Recent research has been focused on essential oils or complex mixtures plants extracts, composed of several secondary metabolites which act in an interesting synergistic way preventing resistance phenomena [23]. Several plants extracts have been obtained, characterized, and investigated as biopesticides [24,25]. Punica granatum peel extract showed high efficiency as natural pesticide to control phytopathogens of crops and postharvest fruits diseases preventing and treating bacteria, fungi, and parasites [26][27][28][29][30].…”

Section: Introductionmentioning

confidence: 99%

Enhanced Bioactivity of Pomegranate Peel Extract following Controlled Release from CaCO₃ Nanocrystals

Baldassarre

Vergaro

Castro

et al. 2022

Bioinorganic Chemistry and Applications

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Pomegranate peel extract is rich of interesting bioactive chemicals, principally phenolic compounds, which have shown antimicrobial, anticancer, and antioxidative properties. The aim of this work was to improve extract’ bioactivity through the adsorption on calcium carbonate nanocrystals. Nanocrystals revealed as efficient tools for extract adsorption reaching 50% of loading efficiency. Controlled release of the contained metabolites under acidic pH has been found, as it was confirmed by quantitative assay and qualitative study through NMR analysis. Specific functionality of inorganic nanocarriers could be also tuned by biopolymeric coating. The resulting coated nanoformulations showed a great antimicrobial activity against B. cinerea fungus preventing strawberries disease better than a commercial fungicide. Furthermore, nanoformulations demonstrated a good antiproliferative activity in neuroblastoma and breast cancer cells carrying out a higher cytotoxic effect respect to free extract, confirming a crucial role of nanocarriers. Finally, pomegranate peel extract showed a very high radical scavenging ability, equal to ascorbic acid. Antioxidant activity, measured also in intracellular environment, highlighted a protective action of extract-adsorbed nanocrystals twice than free extract, providing a possible application for new nutraceutical formulations.

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Anti-fungal activity of phenolic sweet orange peel extract for controlling fungi responsible for post-harvest fruit decay

Cited by 56 publications

References 48 publications

Citrus flavonoids against Fusarium verticillioides in post-harvest maize: Minimization of fumonisins and alteration of fungal ultrastructure

Citrus flavonoids against Fusarium verticillioides in post-harvest maize: Minimization of fumonisins and alteration of fungal ultrastructure

Inhibitory Activity of Shrimp Waste Extracts on Fungal and Oomycete Plant Pathogens

Enhanced Bioactivity of Pomegranate Peel Extract following Controlled Release from CaCO₃ Nanocrystals

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Anti-fungal activity of phenolic sweet orange peel extract for controlling fungi responsible for post-harvest fruit decay

Cited by 56 publications

References 48 publications

Citrus flavonoids against Fusarium verticillioides in post-harvest maize: Minimization of fumonisins and alteration of fungal ultrastructure

Citrus flavonoids against Fusarium verticillioides in post-harvest maize: Minimization of fumonisins and alteration of fungal ultrastructure

Inhibitory Activity of Shrimp Waste Extracts on Fungal and Oomycete Plant Pathogens

Enhanced Bioactivity of Pomegranate Peel Extract following Controlled Release from CaCO3 Nanocrystals

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Product

Resources

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Enhanced Bioactivity of Pomegranate Peel Extract following Controlled Release from CaCO₃ Nanocrystals