Anti-Candida albicansaction of the glyco-protein complex purified from metabolites of gut bacteriumRaoultella ornithinolyticaisolated from earthwormsDendrobaena veneta

Abstract

Aims: The aim of our research was to isolate the compounds from the metabolites of Raoultella ornithinolytica with the activity against Candida albicans and to analyse the action of the compounds on the metabolic activity and morphology of the fungus cells. Methods and Results: The effect of active protein fractions on the cell morphology, growth, and metabolic activity of C. albicans was analysed under a light microscope with Nomarski contrast and after staining with calcofluorwhite. The LIVE/DEAD Yeast Viability Kit F-7030 FUN 1 was used for determination of C. albicans metabolic activity. The biomolecules obtained after isolation by ion exchange chromatography were further fractionated by Sephadex G-50 medium gel filtration. Then, after molecular sieve, the fractions were analysed by FTIR and SERS Spectroscopy. A subfraction was isolated from the antifungal protein fraction above 100 kDa. The active subfraction identified as the glyco-protein complex caused a decrease in the metabolic activity and morphological changes of C. albicans cells. Conclusions: The glyco-protein complex obtained from metabolites of bacteria Raoultella ornithinolytica possesses antifungal activity against C. albicans and shows minimal toxicity (1%) against fibroblasts. Significance and Impact of the Study: Studies on the glyco-protein complex obtained from earthworm gut bacteria R. ornithinolytica can lead to their application in biological fungicide and pharmaceutical industry.

Impact of multi-metals (Cd, Pb and Zn) exposure on the physiology of the yeast Pichia kudriavzevii

“…In this context, FUN-1 has been used in the assessment of the toxic impact of organic and inorganic compounds on yeast metabolic activity (Fiolka et al 2012;ParisiDuchene et al 2006; Van der Heggen et al 2010). Before metal treatment, >97 % yeast cells were metabolically active and thus able to process and concentrate FUN-1 in the vacuole, as orange-red cylindrical intravacuolar structures (CIVS, FUN-1-positive cells) (Fig.…”

Antifungal and anticancer effects of a polysaccharide-protein complex from the gut bacteriumRaoultella ornithinolyticaisolated from the earthwormDendrobaena veneta

“…11856. The SERS analysis confirmed that the compounds obtained are a protein and carbohydrate (Fiołka et al ., ). Our previous analysis performed using SDS/PAGE electrophoresis had shown that the complex obtained contained several high molecular weight proteins (with two main 65‐ and 68‐kDa bands), but after native electrophoresis, one band was observed on the gel.…”

“…After incubation of C. albicans cells with the PPC at the concentration of 5 μg mL −1 , the relative metabolic activity of the fungal cells was significantly decreased by 30% and by 35% after the treatment with 10 μg mL −1 (Fiołka et al ., ). Figure a2,b2 and c2 show metabolically active and inactive cells.…”

“…The antiyeast activity of this complex against C. albicans was observed and demonstrated for the first time by Fiołka et al . ().…”

“…Fluorescent staining for quantification of yeast viability for C. albicans cells had been applied by Fiołka et al . (). Only metabolically active cells were clearly marked with fluorescent intravacuolar structures, while dead cells exhibited extremely bright, diffuse, green yellow fluorescence.…”