Anti-Inflammatory Activities of Kukoamine A From the Root Bark of Lycium chinense Miller

Abstract

Kukoamine A (Kuk A) is a naturally occurring bioactive spermine alkaloid found in the root bark of Lycium chinense, and it exerts various therapeutic effects including antioxidant, neuroprotective, anti-inflammatory, and antidiabetic effects. This study evaluated the anti-inflammatory properties of Kuk A against lipopolysaccharide (LPS)-induced inflammation in RAW 264.7 macrophage cells and carrageenan-induced paw edema in rats. Pretreatment of cells with Kuk A significantly inhibited the production of reactive oxygen species, nitric oxide, prostaglandin E2, cyclooxygenase-2 activity, tumor necrosis factor-α, interleukin-1β (IL-1β), and IL-6 in LPS-treated cells. In addition, pretreatment of rats with Kuk A significantly decreased inflammatory response to carrageenan-induced paw edema by alleviating proinflammatory cytokines in the serum, malondialdehyde levels in the liver and increasing the activities of liver antioxidant enzymes (superoxide dismutase, catalase, and glutathione peroxidase) of carrageenan-treated rats. These results suggest the protective role of Kuk A in acute inflammatory reaction and could be useful in the treatment of inflammatory-related disorders.

Holistic approach to microwave-reflux extraction and thermo-analytical fingerprints of under-utilized Artocarpus heterophyllus seed wastes

“…(2014) investigated the anti-inflammatory properties of Gingerone and their inherent cosmetic use as an anti-ageing agent. Kukoamine A, Euparin and Aloresin are other components with anti-inflammatory properties as reported by Wang et al. (2020) , Xiao et al.…”

Anti-Apoptotic and Anti-Inflammatory Effects of an Ethanolic Extract of Lycium chinense Root against Particulate Matter 10-Induced Cell Death and Inflammation in RBL-2H3 Basophil Cells and BALB/c Mice

“…Of them, five major compounds shown in Figure 8 A, eluted at RT of 5.14 min (P1), 8.37 (P2), 9.61 (P3), 9.98 (P4), and 11.06 (P5), were identified as kukoamine A or kukoamine B (P1), 4-[4-(tert-butoxycarbonyl)piperazin-1-yl]benzoic acid (P2), n -caffeoyltyramine (P3), lyciumin A (P4), and coumarin 314 (P5), respectively ( Figure 8 B and Table 1 ). These compounds, including betaine, have been reported for their anti-inflammatory activities [ 18 , 25 , 26 ], and thus, the observed anti-inflammatory and protective effects of GR30 against PM10-mediated inflammation and basophil cell death could be attributed to these compounds present in GR30.…”

“…Regarding the roots of these traditional medicinal plants, only a small portion of their bark (Lycii Radicis Cortex, LRC) has been traditionally utilized in Korea (under the name of Jigolpi) and China (known as Digupi) for the cure of various symptoms. However, other than the use of their bark (LRC), their whole roots have attracted relatively less attention and have just been discarded as by-products from the Goji farm yards, although they still contain various bioactive substances [ 15 , 16 , 17 , 18 ]. Several recent studies reported that the roots of Lycium species also exhibit various beneficial health effects [ 15 ], such as antioxidant [ 19 ], antitumour [ 20 ], anti-osteoclastic [ 21 ], antimicrobial [ 22 ], and immunomodulatory activities [ 23 ].…”

“…Recently, accumulating evidence showed that the roots of Lycium species also contain various anti-inflammatory metabolites such as kukoamine A [ 18 ], kukoamine B [ 16 ], and lyciumin [ 17 ]. The PMs are now known to induce severe lung inflammation [ 5 , 7 ] and cytotoxicity in many cell types including immune cells [ 4 ].…”

Identification of Chemical Compounds in Ziziphus mauritiana Fruit Juice by GC-MS and LC-MS/MS Analysis

“…While the compounds that were confirmed in the negative mode consisted of 32 compounds, including sanjoinine A, Quinic acid, and Kukoamine A, these three compounds also have biological activity that can contribute medicinally, namely the compound sanjoinine A, which has potential as an antioxidant (Foyet et al, 2019). In addition, Quinic acid compounds also have the potential for hepatoprotective, anti-inflammatory, antioxidant activity and the treatment of dengue virus infection and prostate cancer (Zanello et al, 2015); while the compound kucoamine A has been shown to have antioxidant, anti-inflammatory, and anticancer activities (Li et al, 2017;Wang et al, 2020;Wang et al, 2016).…”