Anti-inflammatory activity of a methanol extract from Ardisia tinctoria on mouse macrophages and paw edema

Abstract. Lagerstroemia ovalifolia Teijsm. & Binn. has traditionally been used as an herbal medicine and possesses anti-inflammatory properties. However, the mechanisms underlying its anti-inflammatory effects remain poorly understood. For this purpose, we aimed to investigate the effects of methanolic extract of L. ovalifolia (LOME) on nitric oxide (NO) and prostaglandin E 2 (PGE 2 ) production, as well as the underlying molecular mechanisms responsible for these effects, in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. We examined the effects of LOME on the production of NO and PGE 2 in LPS-stimulated RAW264.7 cells. To explore the anti-inflammatory mechanisms of LOME, we measured the mRNA or protein expression of the pro-inflammatory mediators induced by LOME in the LPS-stimulated RAW264.7 cells. LOME significantly inhibited the production of NO, PGE 2 , interleukin (IL)-6, IL-1β, and tumor necrosis factor-α (TNF-α) in LPS-stimulated RAW264.7 cells. Moreover, LOME suppressed the mRNA and protein expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and inhibited the phosphorylation of the mitogen-activated protein kinases (MAPKs), with a reduction in the nuclear translocation of nuclear factor (NF)-κB in LPS-stimulated RAW264.7 cells. Taken together, these findings suggest that LOME may exert anti-inflammatory effects in vitro in LPS-stimulated RAW264.7 macrophages and thus, may have potential for use as an adjuvant treatment of inflammatory diseases.

show abstract

“…Among the many pro-inflammatory mediators, NO and PGE 2 play key roles in inflammatory reactions (28,29). NO is Figure 5.…”

Section: Discussionmentioning

confidence: 99%

Amelioration of an LPS-induced inflammatory response using a methanolic extract of Lagerstroemia ovalifolia to suppress the activation of NF-κB in RAW264.7 macrophages

Park

Kwon

Yuniato³

et al. 2016

International Journal of Molecular Medicine

Self Cite

View full text Add to dashboard Cite

show abstract

“…Lipopolysaccharide (LPS) is a potent activator of macrophages, and the activated macrophages produce a variety of proinflammatory molecules, including prostaglandin E 2 (PGE 2 ) and nitric oxide (NO). Among these molecules, inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) are the proinflammatory enzymes necessary for inflammation, and they produce the proinflammatory mediators NO and PGE 2 , respectively [3]. Moreover, LPS stimulation also leads to the generation of inflammatory cytokines such as tumor necrosis factor-α (TNF-α) and interleukin-(IL-) 6 [4].…”

Section: Introductionmentioning

confidence: 99%

Anti-Inflammatory Effects of Antarctic Lichen Umbilicaria antarctica Methanol Extract in Lipopolysaccharide-Stimulated RAW 264.7 Macrophage Cells and Zebrafish Model

Hong

Kim

Min

et al. 2021

BioMed Research International

View full text Add to dashboard Cite

Umbilicaria antarctica (UA) is a member of the family Umbilicariaceae. To the best of our knowledge, no studies on its anti-inflammatory effects have been reported yet. In the present study, we examined its ability to suppress inflammatory responses and the molecular mechanisms underlying these abilities using lipopolysaccharide- (LPS-) stimulated RAW 264.7 cells and a zebrafish model of inflammation. We investigated the effects of UA on the production of nitric oxide (NO) and prostaglandin E2 (PGE2) in LPS-stimulated RAW 264.7 cells. To explore the anti-inflammatory mechanisms of UA, we measured the mRNA and protein expression of proinflammatory mediators in LPS-stimulated RAW 264.7 cells using quantitative RT-PCR and western blot analyses, respectively. UA significantly inhibited the production of NO, PGE2, interleukin- (IL-) 6, and tumor necrosis factor- (TNF-) α in the LPS-stimulated RAW 264.7 cells. It also suppressed the mRNA and protein expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and nuclear factor- (NF-) κB activation in LPS-stimulated RAW 264.7 cells and tail pin-cutting-induced zebrafish model. Collectively, these findings indicate that UA significantly inhibits LPS-stimulated inflammatory responses. These effects were considered to be strongly associated with the suppression of NF-κB activation. Overall, our results demonstrate that UA extract exerts strong anti-inflammatory activities in in vitro and in vivo models and suggest that UA may be an effective novel therapeutic agent for the treatment of inflammatory diseases.

show abstract

“…Several studies have shown that pro-inflammatory mediator expression is regulated by phosphorylation of MAPK family mediators such as ERK 1/2, p38, and JNK (Park et al, 2012). It is well known that LPS drives intracellular signaling pathways such as MAPKs (Kim et al, 2014). Cigarette smoke condensate (CSC) is a strong inducer of the phosphorylation of MAPKs.…”

mentioning

confidence: 99%

Silibinin Inhibits Neutrophilic Inflammation and Mucus Secretion Induced by Cigarette Smoke via Suppression of ERK‐SP1 Pathway

Park

Shin

et al. 2016

Phytotherapy Research

Self Cite

View full text Add to dashboard Cite

Silibinin, the main ingredient of silymarin, has been used as a traditional drug for over 2000 years to treat a range of liver diseases. Recent studies have also demonstrated that silibinin possesses antiinflammatory and anticancer properties. In the study, we researched the efficacy of silibinin on the development of COPD using a cigarette smoke (CS)-induced and lipopolysaccharide (LPS)-induced COPD model mice and stimulation of NCI-H292 cells with CS condensate. Silibinin was administered to mice by oral gavage 1 h before CS exposure for 10 days. In in vitro experiment, we evaluated the effect of silibinin on the expression of MUC5AC in H292 cells stimulated with CS condensate. Furthermore, silibinin suppressed the CS and LPS treatment-induced extracellular signal-regulated kinase (ERK) phosphorylation and SP-1 expression. Silibinin also decreased airway inflammation and reduced the expression of MUC5AC and myeloperoxidase. Furthermore, co-treatment with silibinin and ERK inhibitors considerably decreased the levels of pro-inflammatory mediators, ERK phosphorylation, and SP-1 expression. Taken together, the results indicate that silibinin effectively suppressed the neutrophilic airway inflammation provoked by treatment with LPS and CS, which was closely associated with downregulation of ERK phosphorylation. Therefore, our searching offers that silibinin has a remedical probable for COPD disease. Copyright © 2016 John Wiley & Sons, Ltd.

show abstract

Anti-inflammatory activity of a methanol extract from Ardisia tinctoria on mouse macrophages and paw edema

Cited by 11 publications

References 23 publications

Amelioration of an LPS-induced inflammatory response using a methanolic extract of Lagerstroemia ovalifolia to suppress the activation of NF-κB in RAW264.7 macrophages

Amelioration of an LPS-induced inflammatory response using a methanolic extract of Lagerstroemia ovalifolia to suppress the activation of NF-κB in RAW264.7 macrophages

Anti-Inflammatory Effects of Antarctic Lichen Umbilicaria antarctica Methanol Extract in Lipopolysaccharide-Stimulated RAW 264.7 Macrophage Cells and Zebrafish Model

Silibinin Inhibits Neutrophilic Inflammation and Mucus Secretion Induced by Cigarette Smoke via Suppression of ERK‐SP1 Pathway

Contact Info

Product

Resources

About