Anti-inflammatory activity of probiotic Bifidobacterium: Enhancement of IL-10 production in peripheral blood mononuclear cells from ulcerative colitis patients and inhibition of IL-8 secretion in HT-29 cells

Abstract

AIM:To determine the anti-inflammatory activity of probiotic Bifidobacteria in Bifidobacteria-fermented milk (BFM) which is effective against active ulcerative colitis (UC) and exacerbations of UC, and to explore the immunoregulatory mechanisms. METHODS:Peripheral blood mononuclear cells (PBMNC) from UC patients or HT-29 cells were co-cultured with heat-killed probiotic bacteria or culture supernatant of Bifidobacterium breve strain Yakult (BbrY) or Bifidobacterium bifidum strain Yakult (BbiY) to estimate the amount of IL-10 or IL-8 secreted.RESULTS: Both strains of probiotic Bifidobacteria contained in the BFM induced IL-10 production in PBMNC from UC patients, though BbrY was more effective than BbiY. Conditioned medium (CM) and DNA of both strains inhibited IL-8 secretion in HT-29 cells stimulated with TNF-α, whereas no such effect was observed with heatkilled bacteria. The inhibitory effect of CM derived from BbiY was greater than that of CM derived from BbrY. DNAs of the two strains had a comparable inhibitory activity against the secretion of IL-8. CM of BbiY induced a repression of IL-8 gene expression with a higher expression of IκB-ζ mRNA 4 h after culture of HT-29 cells compared to that in the absence of CM. CONCLUSION:Probiotic Bifidobacterium strains in BFM enhance IL-10 production in PBMNC and inhibit IL-8 secretion in intestinal epithelial cells, suggesting that BFM has anti-inflammatory effects against ulcerative colitis.

Effects of phytochemicals on in vitro anti-inflammatory activity of Bifidobacterium adolescentis

“…Moreover, both lactic acid and acetic acid were found to inhibit the production of pro-inflammatory cytokines, such as tumor necrosis factor-α and interleukin-8, from LPS-stimulated neutrophils and colon tumor cells. 22,29) However, quercetin had no such effect. Therefore, anti-inflammatory substance(s) produced by B. adolescentis during co-culture with the phytochemicals may be neither lactic acid nor acetic acid.…”

“…Phytochemicals, except for quercetin, increased the concentrations of lactic acid and acetic acid in the co-culture medium Since bifidobacterium bacteria is the major producer of lactic acid and acetic acid which were shown to have an anti-inflammatory activity, such as the inhibition of pro-inflammatory cytokine production, 22,29) we next investigated the change of pH and organic acids levels in the co-culture medium. After the culture of B. adolescentis with DMSO or phytochemicals [quercetin, (+)-taxifolin, phloretin, and EGCG] for 3 h, the pH of all of the tested supernatants decreased from 7.8 (just before culture) to 7.2 ( Fig.…”

Modulating the microbiota in inflammatory bowel diseases: prebiotics, probiotics or faecal transplantation?

“…For example, VSL#3 increased the basal luminal mucin content in rats by 60 % and significantly stimulated colonic mucin secretion and MUC2 gene expression in isolated rat colonic loops (62) . Finally, probiotics may affect the mucosal immune system by increasing the production of intestinal anti-inflammatory cytokines such as IL-10 and transforming growth factor β (63)(64)(65) , by modulating the NF-κB pathway (66) and by influencing the cross-talk between natural killer cells and dendritic cells (67) .…”

Effect of Synbiotic Supplementation on Serum Systemic Inflammatory Marker and Serum Albumin in Patients Admitted to ICU

“…Inflammation may reduce serum albumin level and severe hypo-albuminemia are common in critically ill patients [3]. Recently, it is suggested that consumption of probiotics would be a novel approach to decrease inflammation in humans [6,7]. Probiotics are kinds of living microorganisms which have beneficial health effects on humans.…”