Anti-Inflammatory Activity of Silibinin in Animal Models of Chronic Inflammation

Aziz, Tavga Ahmed; Marouf, Bushra Hassan; Ahmed, Zheen Aorahman; Hussain, Saad Abdulrahman

doi:10.12691/ajps-2-1-2

Cited by 8 publications

(4 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The reason behind choosing dexamethasone and prednisolone as standard steroids for comparison; was the fact that each of the aforementioned steroids has been used in similar cases of inflammation. Dexamethasone has been proven to be effective in reducing formalin-induced paw edema and granulomatous inflammation in various animal studies (18,23,24) , and prednisolone appeared to have a successful role in reducing the size and extent of the lesion in patients with idiopathic granulomatous mastitis (25) and patients with solitary cysticercus granuloma (26) . Ginkgo biloba extract for the first time in the current study tested in granulomatous inflammation and was efficacious in producing a significant reduction in the inflammatory response.…”

Section: Discussionmentioning

confidence: 99%

Anti-Inflammatory Activity of Gingko Biloba Extract in Cotton Pellet-Induced Granuloma in Rats: A comparative Study with Prednisolone and Dexamethasone

Kareem¹,

Aziz

Ahmed³

et al. 2022

IJPS

View full text Add to dashboard Cite

The current study was designed to evaluate the anti-inflammatory effect of GKB in the rat model of granulomatous inflammation. Thirty rats were distributed into five groups: The first group served as negative control group that received distilled water (DW) only without inducting inflammation, positive control group; treated with DW with the induction of inflammation and they were assigned to cotton pellet-induced granuloma, ginkgo biloba (GKB) treated group (200mg/kg/day), dexamethasone-treated group (1mg/kg), and Prednisolone treated group (5mg/kg). All the treatments were given orally for seven consecutive days. On day eight, the rats were anesthetized and the pellets together with granulation tissue were carefully removed and made free from extraneous tissue. The weight and the percent of the exudate and granuloma were determined and samples of the tissues were sent for histopathological examination. Blood samples were collected by cardiac puncture and used for the analysis of the inflammatory markers: TNF-alfa, IL10, VCAM-1, and hs-CRP. The study revealed a significant reduction in the weight and the percent of exudate (p-value = 0.019), (17%) and granuloma (p-value = 0.013), (20%) by GKB which was comparable to that produced by prednisolone. All the treatment groups showed a significant reduction in serum TNF-?, VCAM-1, and hs-CRP concentration compared with the positive control. The histopathological finding revealed pronounced improvement. In the current study, GKB was effective in attenuating the level of inflammation by decreasing the exudate, granuloma, and inflammatory markers. The underlying mechanisms could be the inhibitory effect on the expression of the inflammatory cytokines and endothelial adhesion molecule. These findings suggest GKB as a good contender to be tested in the treatment of inflammatory diseases.

show abstract

Section: Discussionmentioning

confidence: 99%

Anti-Inflammatory Activity of Gingko Biloba Extract in Cotton Pellet-Induced Granuloma in Rats: A comparative Study with Prednisolone and Dexamethasone

Kareem¹,

Aziz

Ahmed³

et al. 2022

IJPS

View full text Add to dashboard Cite

show abstract

“…The following formula was used to calculate the amount of exudate, granulation tissue formation in mg, % inhibition of exudate, and granuloma tissue formation [ 60 ]:

…”

Section: Methodsmentioning

confidence: 99%

Evaluation of In Vivo Wound-Healing and Anti-Inflammatory Activities of Solvent Fractions of Fruits of Argemone mexicana L. (Papaveraceae)

Ayele

Abebe

Muche

et al. 2022

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Introduction. The solvent fractions of the fruits of Argemone mexicana L. (Papaveraceae) have not yet been explored scientifically for in vivo wound healing and anti-inflammatory activities. The objective of this study was, therefore, to evaluate in vivo wound healing and anti-inflammatory activities of the solvent fractions of the fruit of Argemone mexicana L. (Papaveraceae) in rats. Method. The crude extract of Argemone mexicana was fractionated with n-hexane, ethyl acetate, and distilled water. Wound healing activity was evaluated using excision and incision wound models while anti-inflammatory activity was evaluated using carrageenan-induced rat paw and cotton pellet-induced granuloma models. The fractions were evaluated at 5 and 10% ointments using moist-exposed burn ointment as the standard drug, and 100, 200, and 400 mg/kg test doses using aspirin, and dexamethasone as standard drugs for wound healing and anti-inflammatory activities, respectively. All treatment administrations were made orally for anti-inflammatory activity and applied topically for wound healing activity. Result. The 10% w/w ethyl acetate fraction ointment showed a significant percentage of wound contraction, reduced period of epithelialization, increased amount of fibrosis, neovascularization, and collagen tissue formation ( p < 0.01 ). The ethyl acetate fraction also showed a significant increase in tensile strength (55%; p < 0.01 ) and (81.10%; p < 0.01 ) at the tested doses of 5 and 10% w/w ointments, which was comparable to moist-exposed burn ointment. The ethyl acetate fraction also revealed a significant percent edema inhibition (61.41%; p < 0.01 ), suppression of the exudate (38.09% p < 0.01 ), and granuloma mass formations (53.47% p < 0.01 ) at the tested dose of 400 mg/kg. Conclusion. The results of this study showed that the Ethyl acetate fraction of Argemone mexicana fruit has significant wound healing and anti-inflammatory activities which support the traditional claims of the experimental plant.

show abstract

“…The exudate amount (mg), granulation tissue formation (mg), and percent inhibition of exudate and granuloma tissue formation were calculated according to the formula given by Aziz et al (2014):…”

Section: Cotton Pellet Induced Granuloma Methodsmentioning

confidence: 99%

Anti-inflammatory activities of ethanol leaves extract and solvent fractions of Zehneria scabra (Cucurbitaceae) in rodents

Belay

Makonnen

2020

Asian J Nat Prod Biochem

View full text Add to dashboard Cite

Abstract. Belay R, Makonnen E. 2018. Anti-inflammatory activities of ethanol leaves extract and solvent fractions of Zehneria scabra (Cucurbitaceae) in rodents. Biofarmasi J Nat Prod Biochem 18: 42-56. Zehneria scarba (L.f.) Sond is one of the medicinal plants used in folkloric medicine of Ethiopia for years to treat various inflammatory disorders. The present study was aimed to validate the anti-inflammatory activity of crude 70% ethanol leaves extract (70EE) against a sub-acute model and further evaluate the solvent fractions (AF, BF, and CF) in an acute (carrageenan-induced paw edema), sub-acute (formaldehyde induced arthritis) and chronic (cotton pellet induced granuloma) inflammatory models. The 70EE was first prepared by maceration, and the fractions were obtained by sequential partitioning with chloroform and n-butanol from the aqueous suspension of crude 70EE. The test groups, then, received 100, 200 and 400 mg/kg of the crude 70EE or the fractions (AF, BF, and CF) at the same dose levels, whereas positive controls received aspirin (200mg/kg) or dexamethasone (0.5mg/kg) and negative controls received vehicle (2% tween 80 or distilled water, 10 mL/kg). All tested doses of the crude 70EE showed significant inhibition of formaldehyde induced arthritis at the 10th day of treatment, on which the 400mg/kg dose showed the maximum anti-arthritic effect (%A = 60.5; p < 0.001). In the carrageenan-induced paw edema, all the three fractions showed a statistically significant effect, in fact, with different onset and magnitude. In this model, the AF was found to be the most active fraction, and the 400mg/kg dose demonstrated the maximum effect (%A = 76.25; p < 0.001) at 5h post-induction, which is much better than the effect of aspirin at the dose employed. The overall order of efficacy in inhibiting the exudative component of carrageenan-induced paw edema was found to be AF> BF> CF. The AF was also found to be the most active fraction in inhibiting the exudative component of chronic inflammation in the cotton pellet induced granuloma model, where the maximum effect (%A = 43.10, p < 0.001) was exhibited by a dose of 400mg/kg. The AF was also the most active fraction in inhibiting formaldehyde induced arthritis, in which the BF and CF relatively showed a comparable effect throughout day 4-10. On the contrary, in the cotton pellet induced granuloma model, the CF was found to be the most active fraction in inhibiting the proliferative and granulomatous component of chronic inflammation, and the overall order of effectiveness was found to be CF> AF> BF. Besides, 400mg/kg of CF demonstrated the maximum inhibition of granuloma formation (%A = 55.52; P < 0.001). The phytochemical analysis revealed the differential distribution of secondary metabolites into the three fractions, which either singly or in concert appeared to be responsible for the observed effects. The data obtained from the present study collectively indicate that the extract and fractions of leaves of Z.scabra possessed a significant anti-inflammatory activity, upholding the folkloric use of the plant.

show abstract

Anti-Inflammatory Activity of Silibinin in Animal Models of Chronic Inflammation

Cited by 8 publications

References 27 publications

Anti-Inflammatory Activity of Gingko Biloba Extract in Cotton Pellet-Induced Granuloma in Rats: A comparative Study with Prednisolone and Dexamethasone

Anti-Inflammatory Activity of Gingko Biloba Extract in Cotton Pellet-Induced Granuloma in Rats: A comparative Study with Prednisolone and Dexamethasone

Evaluation of In Vivo Wound-Healing and Anti-Inflammatory Activities of Solvent Fractions of Fruits of Argemone mexicana L. (Papaveraceae)

Anti-inflammatory activities of ethanol leaves extract and solvent fractions of Zehneria scabra (Cucurbitaceae) in rodents

Contact Info

Product

Resources

About