Anti-inflammatory Effect of Apigenin-7-neohesperidoside (Rhoifolin) in Carrageenin-Induced Rat Oedema Model

Abstract

Flavonoids are normal constituents of the human diet and are known for a variety of biological activities. They have been reported to bring benefits in lowering inflammation and oxidative stress. The present investigation was performed first, to evaluate the anti-inflammatory activity of rhoifolin and second, to search for the possible contributing mechanisms for this hypothesized effect. Rhoifolin caused a time and reverse dose dependent reduction of carrageenin-induced rat paw oedema. Following 4 hr of treatment, rhoifolin at doses 2.5, 25 & 250 mg/kg caused a significant inhibition of rat paw edema volume by 14, 25 & 45 % respectively in comparison to the control group (74%). In addition to significantly abrogating prostaglandin E2 level, increasing doses of rhoifolin significantly diminished the TNF-α release in the inflammatory exudates. In the same animal model, rhoifolin increased the total antioxidant capacity in a reverse dose order, with the highest capacity obtained with the lowest dose tested. This study demonstrates for the first time the effectiveness of rhoifolin in combating inflammation in carrageenin-induced rat oedema model.

Isolation and Identification of a Flavone Apigenin from Marine Red Alga Acanthophora spicifera with Antinociceptive and Anti-Inflammatory Activities

“…The test for oral acute toxicity revealed no mortality or toxicity signs for apigenin at doses up to 5000 mg/kg either in mice or rats. Based on these results, and according to, 24,26 the doses of 25 and 50 mg/kg were selected for further evaluation of antinociceptive and antiinflammatory activities. 30 Analgesic activities.…”

“…The rat doses of apigenin (25 and 50 mg/kg bw) were selected in accordance with those reported previously. 26 The fourth group was given diclofenac sodium (100 mg/kg) and kept as a standard group.…”

Phytochemical study, cytotoxic, analgesic, antipyretic and anti-inflammatory activities of Strychnos nux-vomica

“…It was proven that, flavonoids isolated from some medicinal plants have an antinociceptive and/or antiinflammatory effect (Eldahshan and Azab 2012). Flavonoids inhibit the biosynthesis of prostaglandins, which are involved in various immunologic responses and are the end products of the cyclooxygenase and lipoxygenase pathways (Moroney et al 1988;Di Rosa and Willoughby 1971).…”

Ameliorative Effects of Rhoifolin in Scopolamine-Induced Amnesic Zebrafish (Danio rerio) Model

“…Increasing doses of Rho significantly reduced the tumor necrosis factor-α (TNF-α) release. It also elevated the total antioxidant capacity in a reverse dose order, with the highest ability obtained with the lowest dose tested [ 11 ]. The in vitro study showed that Rho could actively suppress the receptor activators of nuclear factor-kappa B (NF-kB) ligand-stimulated osteoclastogenesis, F-actin formation, hydroxyapatite resorption, and the gene expression of osteoclast-related genes [ 12 ].…”