Anti-Malarial Drug Resistance

Yusuf, Yenni

doi:10.22338/mka.v37.i1.p64-69.2014

Cited by 3 publications

(1 citation statement)

References 12 publications

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“…Treatment of malaria uses ACT (Artemisin-based Combination Therapy) [2]. Resistance to Artemisin has occurred in the Greater Mekong, Southeast Asia in 2011 [3]. In addition, it is also found in several Southeast Asian countries, namely Thailand, Cambodia, and Myanmar [4].…”

Section: Introductionmentioning

confidence: 99%

Potential of Gossypetin Compounds and Organic Acids of Rosella Kombucha Tea as Immunomodulators in Malaria Cases: In Silico Study

Widiani,

Mustika Andini,

Firmansyah

2024

E3S Web of Conf.

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Malaria is an infectious disease caused by Plasmodium. Cases of resistance to Artemisin-based Combination Therapy (ACT) have been found. Therefore, complementary therapy is needed. The content of flavonoids (gossypetin) and organic acids (acetic acid, gluconic acid, and lactic acid) in Rosella Kombucha tea may be used as complementary therapy for the treatment of malaria. The In Silico study used gossypetin, acetic acid, gluconic acid, and lactic acid as ligands. While the target protein is the crystal structure of the enzyme TCR - MHC II Complex. Applications used for docking insilico are AutodockTools 1.5.6, Autodock 4.2 and Discovery Studio Visualizer. The results showed that gossypetin and organic acids had the potential to increase the activity of immunocompetent cells because these compounds were able to bind to the TCR – MHC II complex receptor. Gossypetin is more potent than organic acid compounds. gossypetin has Free Energy Binding (∆Gbinding) value (-2.26 kcal/mol), inhibition constant value (22.21 nM) and RMSD value < 5 (2.58). Meanwhile, the most potent organic acid was lactic acid with a value of Free Energy Binding (∆Gbinding) (-1.76 kcal/mol), an inhibition constant value (51.69 nM) and an RMSD value < 5 (4.1).

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Section: Introductionmentioning

confidence: 99%

Potential of Gossypetin Compounds and Organic Acids of Rosella Kombucha Tea as Immunomodulators in Malaria Cases: In Silico Study

Widiani,

Mustika Andini,

Firmansyah

2024

E3S Web of Conf.

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Analisis in Silico Senyawa Hibiscetin Kombucha Rosella Sebagai Imunomodulator Sel Imunokompeten Pada Penyakit Malaria

Widiani¹,

Winandari

Kamelia

et al. 2021

BJPB

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Malaria is one of the public health problems in Indonesia. The data show that drug resistance is found in malaria control. For this reason, various efforts are needed to find solutions to overcome these problems. The presence of the flavonoid compound hibiscetin in Kombucha Rosella is thought to act as an immunomodulator that can increase the activity and proliferation of lymphocyte cells during malaria infection. Kombucha Rosella is considered to be a supportive therapy for malaria treatment. The purpose of this study was to obtain a scientific basis for the use of Kombucha Rosella tea as immunotherapy for malaria. The method used is by using An silico docking simulation. The ligand compound used was hibiscetin and the target protein was TCR -MHC II complex. Docking simulation using AutodockTools 1.5.6, Autodock 4.2, and Discovery Studio Visualizer applications. The results showed that the Hibisctin compound had the potential to increase the activity of immunocompetent cells in silico with the value of Free Energy Binding (∆Gbinding) -3.08, the value of the inhibition constant was 5.49 nM, and the RMSD value <5 was 3.32.

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Anti-malarial activity and toxicity of Aspidosperma nitidum Benth: a plant used in traditional medicine in the Brazilian Amazon

Brandão

Silva

Almeida

et al. 2020

RSD

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The objective of this work was to evaluate the antiplasmodial activity and toxicity of the extract and fractions obtained from the bark of Aspidosperma nitidum. The ethanol extract obtained from the powdered bark of plants was acid-base partitioned and phytochemically analyzed. The antiplasmodial activity, in vivo antimalarial activity and in vitro cytotoxicity were acessed. The selectivity index (SI) was calculated. The acute oral toxicity and pathological effects, of the ethanol extract was evaluated in mice. The major constituent of the ethanol extract was suggestive of a β-carboline chromophore. The alkaloid and neutral fractions contained compounds with an aspidospermine core as the major constituent. The ethanol extract (IC50 = 3.60 µg/mL), neutral fraction (IC50 = 3.34 µg/mL) and alkaloid fraction (IC50= 2.32 µg/mL) showed high activity against P. falciparum (W2 strain). The ethanol extract and the alkaloid fraction reduced 80% of the parasitemia of P. berghei (ANKA)-infected mice (dose of 500 mg/kg) in the 5th day, which was not sustainable at the 8th day. A similar result was obtained for chloroquine. The ethanol extract (CC50 = 410.65 µg/mL; SI = 114.07), neutral fraction (CC50 = 452.53 µg/mL; SI = 135.49), and alkaloid fraction (CC50 =346.73 µg/mL; SI 149.45) demonstrated low cytotoxicity and high SI. The ethanol extract (5000 mg/kg; gavage) presented low acute oral toxicity, with no clinical or anatomopathological modifications being observed (in comparison to the control group). In vitro studies with a chloroquine-resistant clone of P. falciparum confirmed the antiplasmodial activity of the A. nitidum ethanol extract, and its fractions had low cytotoxicity for HepG2 cells. In vivo studies with P. berghei–infected mice and acute toxicity studies corroborated these results.

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Anti-Malarial Drug Resistance

Cited by 3 publications

References 12 publications

Potential of Gossypetin Compounds and Organic Acids of Rosella Kombucha Tea as Immunomodulators in Malaria Cases: In Silico Study

Potential of Gossypetin Compounds and Organic Acids of Rosella Kombucha Tea as Immunomodulators in Malaria Cases: In Silico Study

Analisis in Silico Senyawa Hibiscetin Kombucha Rosella Sebagai Imunomodulator Sel Imunokompeten Pada Penyakit Malaria

Anti-malarial activity and toxicity of Aspidosperma nitidum Benth: a plant used in traditional medicine in the Brazilian Amazon

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