Anti-microbial efficacy and mode of action studies on a new zinc/Triclosan formulation

Abstract

Objective:To test in vitro the anti-plaque/ antimicrobial efficacy of a new toothpaste formulation containing a 2% zinc citrate/ 0.3% Triclosan antimicrobial system compared with a 0.75% zinc citrate/ 0.3% Triclosan system and where appropriate, against controls of a standard fluoride paste and a 0.3% Triclosan/ 2% copolymer product. Methods: The anti-metabolic activity was assessed using a range of assays measuring the ability of the active systems to inhibit bacterial glycolysis. The antibacterial/ antiplaque activity was assessed in an in vitro multispecies biofilm assay. Results: Both zinc formulations were shown to have significantly superior activity at inhibiting glycolysis compared with the 0.3% Triclosan/ 2% copolymer formulation and the standard fluoride paste , particularly in reducing the pH drop after sugar challenge, the new formulation having the greatest activity. Likewise, in the antibacterial assay , both zinc formulations were found to have significantly superior activity over a standard fluoride paste and the 2% zinc citrate/ 0.3% Triclosan formulation was shown to be significantly better than 0.75% zinc citrate/ 0.3% Triclosan formulation . Conclusion : These data provide support for the enhanced performance of the 2% zinc citrate/ 0.3% Triclosan formulation .

Effect of copper and zinc on microbial tolerance to triclosan in two soil types

“…Horswell et al (2014) suggested that high concentrations of zinc or copper inhibited the ability of soil microbes to degrade triclosan thereby causing a greater effect when both zinc and triclosan were present. In both oral and nutrient-rich soil environments, similar interaction effects were observed whereby microbial biomass and metabolic activities were more affected when microbial communities were exposed to a combination of zinc and triclosan than each individually (Brading et al 2003).…”

The antibacterial effects of zinc ion migration from zinc-based glass polyalkenoate cements

“…As zinc inhibits multiple activities in the bacterial cell, such as glycolysis, transmembrane proton translocation and acid tolerance [14], it has been shown to exhibit an antibacterial effect at considerably lower concentrations than many antimicrobial agents [32]. Furthermore, though generally regarded as bacteriostatic, it can have bactericidal effects also, particularly when used in combination with other ions, such as fluoride [33], or antibacterial agents [34].…”

Glass-ionomer Cement SiO2, Al2O3, Na2O, CaO, P2O5, F- Containing Alternative Additive of Zn and Sr Prepared by Sol–gel method

“…Phan et al (34) studied the antibacterial effect of Zn 2+ ions and suggested that zinc inhibits multiple activities in the bacterial cell, such as glycolysis, transmembrane proton translocation and acid tolerance, it has been shown to exhibit an antibacterial effect at considerably lower concentrations than many antimicrobial agents (35) . Nevertheless, the cements without Sr and with different Zn content exhibited different inhibition of both bacterial species.…”