Anti-Neospora caninum antibodies among dairy cattle in a rural settlement, Paraná, Brazil

Katto, Shiguedy; Filho, Luiz Fernando Coelho da Cunha; Bogado, Alexey Leon Gomel; Martins, Thais Agostinho; Sammi, Ana Sue; Santos, Joeleni Rosa dos; Kono, Isabelli Sayuri; Seixas, Mércia de; Nino, Beatriz de Souza Lima; Garcia, João Luís

doi:10.5433/1679-0359.2017v38n1p259

Cited by 2 publications

(4 citation statements)

References 15 publications

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“…Eighteen months after the first study, we performed a second sampling from 68 cows that were sampled before, and it was observed an incidence of antibodies of 7.4%, corroborating the data showed by Katto et al (2017) who reported an incidence of 7.7%.…”

Section: Discussionsupporting

confidence: 86%

“…In addition, the fluctuation of antibodies may influence the seropositivity for N. caninum of the herds over time. Katto et al (2017) detected 23.1% of seropositivity in cattle that changed to 15.5% after 2 years.…”

Section: Discussionmentioning

confidence: 94%

“…The determination of anti-N. caninum IgG antibody titers was performed using the indirect immunofluorescence (IFI) technique according to Conrad et al (1993). The slides for antibody detection were prepared with N. caninum tachyzoites (strain NC-1), and samples with titers ≥ 100 were considered positive for bovines (CARDOSO et al, 2012;KATTO et al, 2017), while titers ≥ 50 were considered positive for dogs (MASCOLLI et al, 2015). Positive and negative controls were used at each slide.…”

Section: Serological Testingmentioning

confidence: 99%

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Neospora caninum: soroepidemiologia de vacas e cães de propriedades leiteiras de Cunha, São Paulo, Brasil

Bastos

Martins

Sammi

et al. 2019

SCA

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Neosporosis, a parasitic disease prevalent worldwide, is caused by the protozoan Neospora caninum, an obligatory intracellular parasite that can cause abortions and infertility in females, resulting in high economic losses. The aim of this study was to determine the seroprevalence of antibodies against N. caninum in dairy cattle, and the occurrence of antibodies in dogs from farms in the municipality of Cunha, São Paulo, Brazil. Additionally, eighteen months after the primary study, an analysis to determine the incidence, seroconversion, and occurrence of anti-N. caninum antibodies was conducted in cows. Sampling was carried out in two phases: during the first one, 400 dairy cattle and 50 dog blood samples from 40 farms were collected. During the second phase, blood samples were collected from 119 cows belonging to 16 farms visited 18 months before. Serum samples were analyzed by indirect immunofluorescence (IFI) with titers ? 100 for cattle and ? 50 for dogs considered as positives. The occurrence of antibodies against N. caninum was 15.0% (60/400) and 14.0% (7/50) for cattle and dogs, respectively. After 18 months, the observed incidence of antibodies was 7.4% (4/54), the conversion detected was 71.4% (seropositive to seronegative) and 7.4% (seronegative to seropositive animals), and the observed occurrence was 7.56% (9/119). The calculated odds ratio (OR) was significant (p < 0.05) for abortions, presence of dogs, and parturition stalls. However, protection factors were identified in 2- to 4-year-old dairy cattle and in the number of lactating cows ? 10 animals. Thus, the presence of antibodies against N. caninum in the studied suggested both vertical and horizontal transmission are occurring in cattle.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 94%

Section: Serological Testingmentioning

confidence: 99%

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Neospora caninum: soroepidemiologia de vacas e cães de propriedades leiteiras de Cunha, São Paulo, Brasil

Bastos

Martins

Sammi

et al. 2019

SCA

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“…The seroprevalence observed for N. caninum in this study was 41.6%, while the seroprevalence of N. caninum obtained via IFA ranged from 14.1% to 97.2% in Brazil ( Guimarães et al, 2004 ; Albuquerque et al, 2005 ; Ogawa et al, 2005 ; Benetti et al, 2009 ; Camillo et al, 2010 ; Silva et al, 2015 ; Chiebao et al, 2015 ; Klauck et al, 2016 ; Katto et al, 2017 ; Fávero et al, 2017 ; Snak et al, 2018 ; Bastos et al, 2019 ; Santos et al, 2020 ; Azevedo et al, 2021 ; Souza et al, 2022 ) and 12.4% to 43.1% in other parts of the world ( Moore et al, 2002 , 2009 ; Puray et al, 2006 ; González-Warleta et al, 2008 ; Eiras et al, 2011 ; Qian et al, 2017 ; Serrano-Martínez et al, 2007 ). Some of these studies used different cut-offs in their IFA, and several studies that involved the use of IFA as a serological technique have been conducted in Brazil.…”

Section: Discussionmentioning

confidence: 52%

Seroprevalence and risk factors for Neospora caninum and Toxoplasma gondii in dairy cattle from São Paulo State, Brazil

Bernardes,

Pinto-Ferreira,

Ladeia

et al. 2024

Rev. Bras. Parasitol. Vet.

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Neospora caninum is a major cause of reproductive loss in cattle worldwide as it leads to abortion and animal repositioning. Although Toxoplasma gondii does not cause a reproductive problem in cattle, consuming raw or uncooked beef poses the risk of transmission. This study aimed to evaluate the occurrence of anti-N. caninum and anti-T. gondii antibodies in dairy cattle in the West and Northwest regions of São Paulo State, Brazil. A total of 653 serum samples from dairy cows were analyzed using an indirect immunofluorescence assay (IFA). Epidemiological data from the farms were associated with the serological results of the animals by logistic regression based on the presence of antibodies. The frequencies of the antibodies against N. caninum and T. gondii were 41.6% (272/653) and 11.5% (75/653), respectively. A statistically significant association was observed between: the serum anti-N. caninum antibodies and breed, history of food supplementation for calves, introduction of outside animals that later presented reproductive problems, and history of reproductive problems by the trimester of gestation. The present study highlights the importance of neosporosis in dairy cattle in the study regions and that the inclusion of this parasite in the investigation of animals with reproductive disorders is important.

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Anti-Neospora caninum antibodies among dairy cattle in a rural settlement, Paraná, Brazil

Cited by 2 publications

References 15 publications

Neospora caninum: soroepidemiologia de vacas e cães de propriedades leiteiras de Cunha, São Paulo, Brasil

Neospora caninum: soroepidemiologia de vacas e cães de propriedades leiteiras de Cunha, São Paulo, Brasil

Seroprevalence and risk factors for Neospora caninum and Toxoplasma gondii in dairy cattle from São Paulo State, Brazil

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