Anti-Platelet Activity of Water Dispersible Curcuminoids in Rat Platelets

Abstract

Curcuminoids are active principle of turmeric with plethora of health beneficial properties. In this study, we have evaluated for the first time the effect of water dispersible curcuminoids on rat platelet aggregation. Curcuminoids (10-30 µg/mL) significantly inhibited platelet aggregation induced by agonists viz., collagen, ADP and arachidonic acid. Curcuminoids were found to be two-fold more potent than curcumin in inhibiting platelet aggregation. Intracellular curcuminoid concentration was relatively higher than curcumin in rat platelets. Curcuminoids significantly attenuated thromboxane A2 , serotonin levels in rat platelets which play an important role in platelet aggregation. Curcuminoid treatment increased nitric oxide (NO) levels in platelets treated with agonists. Curcuminoids inhibited free radicals such as superoxide anion released from activated platelets, which ultimately inhibits platelet aggregation. Further, curcuminoids inhibited 12-lipoxygenase activity and formation of 12-hydroperoxyeicosatetraenoic acid (12-HPETE) in activated rat platelets which regulates platelet aggregation. The results suggest that curcuminoids have remarkable anti-platelet activity by modulating multiple mechanisms involved in platelet aggregation. Thus curcuminoids may have a therapeutic potential to prevent platelet activation related disorders.

The in vitro bioavailability of anti‐platelet peptides in collagen hydrolysate from silver carp ( Hypophthalmichthys molitrix ) skin

“…Considering the important role of platelet release, the markers of platelet release were measured by the corresponding ELISA kit. The released 5‐HT, rich in the dense granule (Maheswaraiah, Jaganmohan, & Naidu, 2015), had no significant difference neither the ADP treatment nor oligopeptides incubation ( p > .05, data not shown). However, the released β‐TG, rich in the α‐granule (Heijnen & Sluijs, 2015) were significantly enhanced by GPR pretreatment (Figure 4d).…”

Inhibition of thrombin, an unexplored function of retinoic acid

“…A steep decrease in absorbance was observed when platelet aggregation occurred. This decrease is less when the test compound inhibits platelet aggregation [ 41 ]. The mean values of percentage inhibition of platelet aggregation (with reference to control – DMSO) are presented.…”

The regulatory role of curcumin on platelet functions

“…Collectively, curcuminoids showed noticeable antiplatelet action through modulating different mechanisms involved in platelet aggregation. Hence, curcuminoids, as potential therapeutic agents, might be able to preserve platelet activation‐related diseases . Dehydrozingerone, a natural phenolic ingredient extracted from the rhizomes of Zingiber officinale , is a half structural analog of curcumin and one of the recognized degradants of it.…”