Anti-pseudomonal Effect of a Nephrite-containing Contact Lens Storage Case

Abstract

Purpose:To compare the anti-pseudomonal effect of nephrite-containing contact lens (CL) storage cases with conventional CL storage cases. Methods: The degree of proliferation and adhesion of Pseudomonas aeruginosa (P. aeruginosa) on 1st-3rd generation silicone hydrogel contact lenses (SHCLs) was compared by using both 5% nephrite-containing CL storage cases and conventional CL storage cases. The degree of adhesion of P. aeruginosa to the CL was also compared in 1% or 5% nephrite solution and multipurpose solution (MPS). Results: Nephrite-containing CL storage cases showed a significant inhibitory effect on P. aeruginosa proliferation (p = 0.03) and significantly reduced the number of adherent P. aeruginosa on all 1st-3rd generation SHCLs compared with conventional CL storage cases (p = 0.02, p = 0.03, and p < 0.01, respectively). There was no significant anti-pseudomonal effect of 1% and 5% nephrite solutions compared with MPS. Conclusions: Nephrite may be the next generation substance that reduces infectious keratitis caused by P. aeruginosa when added to CL storage cases. J Korean Ophthalmol Soc 2018;59(8):724-729

Antibacterial Activity of Antibiotic-Releasing Polydopamine-Coated Nephrite Composites for Application in Drug-Eluting Contact Lens

“…Since nephrite itself has antibacterial activity against Gram-negative or Gram-positive bacteria, nephrite was selected as an antibacterial material for SCL [ 13 , 14 , 15 ]. Additionally, PDA was coated onto the surface of nephrite to emphasize antibacterial activity because it is frequently employed as an antibacterial coating material for various implants [ 22 ].…”

“…Nephrite is a mineral tremolite or actinolite which is composed of calcium, magnesium and iron. In particular, we previously reported the antibacterial activity of nephrite against Gram-positive or Gram-negative bacteria [ 13 , 14 , 15 ]. We reported that the storage case for nephrite-containing contact lenses showed an inhibitory effect on the proliferation of S. aureus and thus nephrite can be used as a novel antibacterial material for keratitis [ 13 ].…”

“…We reported that the storage case for nephrite-containing contact lenses showed an inhibitory effect on the proliferation of S. aureus and thus nephrite can be used as a novel antibacterial material for keratitis [ 13 ]. They also showed antibacterial activity against pseudomonas aeruginosa ( P. aeruginosa ) [ 14 ]. Additionally, storage cases for nephrite-containing contact lenses showed an inhibitory effect against Acanthamoeba lugdunensis ( A. lugdunensis ) [ 15 ].…”