Anti Pseudorabies Virus Activity of Kumazasa Extract

Iwata, Kei; Naito, Erika; Yamashita, Kazutaka; Kakino, Kenichi; Taharaguchi, Satoshi; Kimachi, Yoshihisa; Hara, Motonobu; Takase, Kozo

doi:10.4265/bio.15.123

Cited by 12 publications

(7 citation statements)

References 25 publications

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“…Propolis flavone and Epimedium polysaccharide synergistically inhibit the cellular infectivity of Newcastle disease virus (NDV) and improved the treatment of Newcastle Disease in chicken (Fan et al, 2011). Kumazasa extract had the antipseudorabies virus activity (Iwata et al, 2010). Cryptoporus volvatus extract inhibited PRRSV in vitro and in vivo (Gao et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…Chinese traditional herbal medicines could not only directly inactivate the virus, but also enhance the antivirus ability of immune system. Therefore, increasingly attention was gained on Chinese traditional medicines or their extractive antiviral function such as Cryptoporus volvatus, Kumazasa, Baicalin, Tea seed saponins and Sodium tanshinone IIA sulfonate etc (Iwata et al, 2010;Gao et al, 2013;Li et al, 2015;Zhu et al, 2015;Wang et al, 2016). Matrine, an alkaloid that is extracted from Sophora flavescens Ait, has a lot of biological activities (Sun et al, 2014;Yong et al, 2015;Wu et al, 2016).…”

Section: Research Articlementioning

confidence: 99%

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Matrine Inhibits Replication of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) by Influencing the Activation of Nsp9

Sun¹

2018

PVJ

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Matrine is the major active compound found in the root of Chinese herbal medicine Sophora flavescens and has a wide range of pharmacological efficacies. Our previous studies showed that Matrine could inhibit the replication of porcine reproductive and respiratory syndrome virus (PRRSV) directly. However, the underlying inhibition mechanism remains unknown. The main aim of this study was to explore the inhibitory mechanism of Matrine in PRRSV replication in vitro. The mRNA expression of PRRSV N gene was determined using RT-qPCR. The expression of pERK1/2 protein was evaluated using Western blot and the effect of Matrine on PRRSV Nsp9 activity was studied using dual luciferase reporter gene assay. The results showed that Matrine suppress the mRNA transcription of PRRSV N gene in Marc-145 cells at 3 h and 24 h post PRRSV infection and had no inhibitory effect on the ERK1/2 signal pathway activated by PRRSV. The results of dual luciferase reporter gene assay showed that Matrine suppressed the interaction of Nsp9 with promoter p107. Taken into account the results above, our study demonstrated that Matrine inhibit the activity of Nsp9 to suppress the virus replication.

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Section: Discussionmentioning

confidence: 99%

Section: Research Articlementioning

confidence: 99%

Matrine Inhibits Replication of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) by Influencing the Activation of Nsp9

Sun¹

2018

PVJ

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“…Nowadays, people pay close attention to Chinese herb medicines for combating diseases including antiviral activity. It has been reported that the extracts of Kumazasa, Solanum nigrum, Myrothamnus flabellifolia Welw and Alpinia katsumadai had anti-infection properties against Pseudorabies virus, hepatitis C virus, herpes simplex virus type 1 and rotaviruses, respectively (Gescher et al, 2011;Iwata et al, 2010;Javed et al, 2011;Kim et al, 2012), but these crude extracts from Chinese herb medicine possessed complex or undefined constituents which hampered the development of new antiviral drugs. In this experiment, we challenged the CEF cells with IBDV and observed the anti-IBDV activity of 20 constituents derived from Chinese herb medicines.…”

Section: Discussionmentioning

confidence: 99%

“…It has been proved that Chinese herb medicines can provide valuable materials for the discovery and development of new drugs, and clinical trials have shown that some Chinese herb medicines and their ingredients have strong antiviral activity in human and animal viral infection (Fan, 2011;Iwata, 2010). In this study, we investigated the anti-IBDV activity of 20 constituents derived from Chinese herb medicines as an attempt to search for the most promising anti-IBDV drugs.…”

Section: Introductionmentioning

confidence: 99%

Antiviral effects of the constituents derived from Chinese herb medicines on infectious bursal disease virus

Sun

Song

Niu

et al. 2013

Pharmaceutical Biology

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Context: The prevalence of infectious bursal disease has brought about enormous financial losses to the world poultry industry. Chinese herb medicines can provide valuable materials for discovery and development of new drugs. Objective: To screen constituents derived from Chinese herb medicines for their antiviral activity against infectious bursal disease virus (IBDV) in vitro. Materials and methods: Twenty constituents derived from Chinese herb medicines and B87 strain of IBDV were used. The 50% cytotoxic concentration (CC 50 ) and 50% effective concentration (EC 50 ) were determined by visualization of cytopathologic effect (CPE) and 3-(4,5-dimethyithiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) test on chicken embryo fibroblast. Selectivity index (SI) and inhibition ratio (%I) were calculated from the data obtained from the MTT test. Results: Antiviral assays showed dipotassium glycyrrhizinate and ligustrazine hydrochloride among the 20 constituents tested exhibited significant inhibitory activity against IBDV in a dose-dependent manner. EC 50 of dipotassium glycyrrhizinate and ligustrazine hydrochloride were 663.2 AE 268.4 and 92.52 AE 21.13 mg/mL, and SI were 44.52 and 421.62, respectively. The time-of-addition and virucidal assay indicated that anti-IBDV activity of the two constituents could be due to their inhibiting virus replication and/or inactivating virus directly. The inhibition of virus attachment was not observed in the adsorption inhibition assay. Dipotassium glycyrrhizinate and ligustrazine hydrochloride exhibited more than 70% and 80% inhibition of IBDV, respectively, at the maximum safe concentration. Discussion and conclusion: We believe that dipotassium glycyrrhizinate and ligustrazine hydrochloride can be used to develop a new anti-IBDV compound, and it is worth applying the constituents in clinical practice.

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“…The leaves contain lignin, polysaccharides, and chlorophyll, among other components [ 12 , 13 ], and are used as a folk medicine for stomachaches. Recently, Kumazasa extract has been observed to have various pharmacological effects, such as antioxidant [ 14 ], anti-inflammatory [ 15 , 16 ], antibacterial [ 17 , 18 ], and anticancer effects [ 19 ], and to be useful as a health food material. In addition, Kumazasa extract has been reported to have a wound healing effect [ 20 ], and its effect on the skin is also attracting attention.…”

Section: Introductionmentioning

confidence: 99%

Wound-Healing and Skin-Moisturizing Effects of Sasa veitchii Extract

et al. 2021

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Sasa veitchii (S. veitchii) is a traditional herb derived from the bamboo genus, which is collectively called Kumazasa. Although Kumazasa extract is believed to have various effects on the skin, there is little scientific evidence for these effects. In this study, we aimed to obtain scientific evidence regarding the wound-healing and skin-moisturizing effects of Kumazasa extract. Kumazasa extract was applied to the skin of a mouse wound model for 14 days, and the wound area and dermal water content were measured. Mice treated with Kumazasa extract had smaller wound areas than control mice. The dermal water content in the Kumazasa extract-treated group was significantly higher than that in the control group. The mRNA and protein expression levels of cutaneous aquaporin-3 (AQP3), which is involved in wound healing and increases in dermal water content, were significantly increased by treatment with Kumazasa extract. Kumazasa extract-treated HaCaT cells exhibited significantly higher AQP3 expression and p38 mitogen-activated protein kinase (MAPK) phosphorylation than control cells. With continuous application, Kumazasa extract increases AQP3 expression and exerts wound-healing and moisturizing effects. The increase in AQP3 expression elicited by Kumazasa extract may be due to enhancement of transcription via activation of p38 MAPK signaling.

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Anti Pseudorabies Virus Activity of Kumazasa Extract

Cited by 12 publications

References 25 publications

Matrine Inhibits Replication of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) by Influencing the Activation of Nsp9

Matrine Inhibits Replication of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) by Influencing the Activation of Nsp9

Antiviral effects of the constituents derived from Chinese herb medicines on infectious bursal disease virus

Wound-Healing and Skin-Moisturizing Effects of Sasa veitchii Extract

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