Anti-virulence activity of polyphenolic fraction isolated from Kombucha against Vibrio cholerae

Bhattacharya, D.P.; Sinha, Ritam; Mukherjee, Priyadarshini; Howlader, Debaki Ranjan; Nag, Dhrubajyoti; Sarkar, Soumyadev; Koley, Hemanta; Withey, Jeffrey H.; Gachhui, Ratan

doi:10.1016/j.micpath.2019.103927

Cited by 29 publications

(23 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, research should shift the focus to studying antimicrobial properties of phenolics in vivo (92)(93)(94)(95)(96)(97)(98)(99)(100)(101). More animal and human experiments should be conducted in this field to establish general principles affecting their absorption in vivo.…”

Section: Other Considerations and Future Needsmentioning

confidence: 99%

Challenges to antimicrobial susceptibility testing of plant-derived polyphenolic compounds

Bubonja‐Šonje

Knežević

Abram

2020

Archives of Industrial Hygiene and Toxicology

View full text Add to dashboard Cite

As multidrug resistance gains momentum, the last two decades have seen an ever-growing interest in the antimicrobial properties of plant extracts and plant-derived compounds. Most of the focus is on polyphenols – a large and diverse group of phytochemicals with strong antibacterial activity. Testing methods provide reliable results as long as they follow standard procedures. However, methods and procedures used in antimicrobial susceptibility testing (AST) are often too diverse to allow comparison of results. The lack of uniformity and comparability is much owed to the absence of guidelines. The focus of this review is to give a critical overview of different methods used in the assessment of polyphenols antimicrobial efficacy and to highlight the importance of their standardisation.

show abstract

Section: Other Considerations and Future Needsmentioning

confidence: 99%

Challenges to antimicrobial susceptibility testing of plant-derived polyphenolic compounds

Bubonja‐Šonje

Knežević

Abram

2020

Archives of Industrial Hygiene and Toxicology

View full text Add to dashboard Cite

show abstract

“…Antioxidant (Cardoso et al., 2020; Lobo et al., 2017), antimicrobial (Ayed et al., 2017, 2020), antivirulence (Bhattacharya et al, 2020), hepatoprotective (Bhattacharya et al., 2011), antihyperglycemic (Srihari et al., 2013), and anti‐inflammatory (Vázquez‐Cabral et al., 2017) bioactivities were observed in kombucha. They are related to the chemical composition of the medium (Jayabalan et al., 2014).…”

Section: Introductionmentioning

confidence: 99%

Antioxidant and antibacterial activity of a beverage obtained by fermentation of yerba‐maté ( Ilex paraguariensis ) with symbiotic kombucha culture

Lopes

Santos

Prentice

2020

J. Food Process. Preserv.

View full text Add to dashboard Cite

This study aimed to characterize yerba‐maté kombucha by showing its oxidative stress inhibitory property with Saccharomyces cerevisiae yeast as the model and to determine its antibacterial activity against Staphylococcus aureus and Escherichia coli. Different yerba‐maté concentrations and fermentation temperatures were analyzed by an experimental design. Sucrose was used as the carbon source and green tea kombucha was used as the control. Yerba‐maté beverage, which was fermented for 12 days had pH levels between 1.8 and 2.3; acidity from 4.8% to 6.0% acetic acid; total reducing sugars between 15.9 and 23.1 g/L; and total phenolic compounds (TPC) between 444.2 and 844.8 mg/L eq. gallic acid. Antioxidant activity, measured by the ABTS+ method, was from 16.1% to 31.3% higher than unfermented extracts. Yerba‐maté kombucha exhibited antibacterial activity and was also effective to prevent the oxidative stress in S. cerevisiae. Practical applications Yerba‐maté kombucha contains bioactive compounds that have potential applications in the food industry. Polyphenols are the main compounds found in yerba‐maté kombucha, whose properties, such as antioxidant activity demonstrated in in vitro and in vivo tests, are potentially beneficial to health. Organic acids produced during fermentation to inhibit bacterial growth increase shelf life of the beverage and may be used to preserve other food. Thus, due to its significant potential, this study aimed to evaluate different yerba‐maté concentrations and fermentation temperatures to obtain kombucha with high bioactive properties.

show abstract

“…In this case, small molecules present in the beverage were shown to inhibit motility, alter motility gene expression and inhibit mucin penetration in vitro . 59 The idea of developing new therapeutic alternatives for cholera based on the gut microbiota is particularly enticing in the context of this work, since the V. cholerae strain used herein is resistant to multiple antibiotics, and a non-antibiotic therapeutic intervention would be widely useful during infections with drug-resistant organisms. However, further studies will be required to address the therapeutic value of such compounds, and to reveal the details of interspecies interactions in the human microbiota and how they contribute to the balance between host colonization resistance and susceptibility.…”

Section: Discussionmentioning

confidence: 99%

Bioactive small molecules produced by the human gut microbiome modulate Vibrio cholerae sessile and planktonic lifestyles

et al. 2021

View full text Add to dashboard Cite

Humans live in symbiosis with a diverse community of microorganisms, which has evolved to carry out many specific tasks that benefit the host, including protection against invading pathogens. Within the chemical diversity of the gastrointestinal tract, small molecules likely constitute chemical cues for the communication between the microbiota and pathogens. Therefore, we sought to investigate if molecules produced by the human gut microbiota show biological activity against the human pathogen Vibrio cholerae. To probe the effects of the gut metabolome on V. cholerae, we investigated its response to small-molecule extracts from human feces, from a complex bacterial community cultivated in vitro, and from culture supernatants of Enterocloster citroniae, Bacteroides thetaiotaomicron, and Bacteroides vulgatus. Using RNA sequencing, we determined the impact of the human gut metabolome on V. cholerae global gene expression. Among the genes downregulated in the presence of the fecal extract, the most overrepresented functional category was cell motility, which accounted for 39% of repressed genes. Repression of V. cholerae motility by the fecal extract was confirmed phenotypically, and E. citroniae extracts reproduced this phenotype. A complex in vitro microbial community led to increased motility, as did extracts from B. vulgatus, a species present in this community. Accordingly, mucin penetration was also repressed by fecal and E. citroniae extracts, suggesting that the phenotypes observed may have implications for host colonization. Together with previous studies, this work shows that small molecules from the gut metabolome may have a widespread, significant impact on microbe-microbe interactions established in the gut environment.

show abstract

Anti-virulence activity of polyphenolic fraction isolated from Kombucha against Vibrio cholerae

Cited by 29 publications

References 39 publications

Challenges to antimicrobial susceptibility testing of plant-derived polyphenolic compounds

Challenges to antimicrobial susceptibility testing of plant-derived polyphenolic compounds

Antioxidant and antibacterial activity of a beverage obtained by fermentation of yerba‐maté ( Ilex paraguariensis ) with symbiotic kombucha culture

Bioactive small molecules produced by the human gut microbiome modulate Vibrio cholerae sessile and planktonic lifestyles

Contact Info

Product

Resources

About