Anti-virulence properties of catechin-in-cyclodextrin-in-phospholipid liposome through down-regulation of gene expression in MRSA strains

Sinsinwar, Simran; Jayaraman, Adithyan; Mahapatra, Santanu Kar; Vadivel, Vellingiri

doi:10.1016/j.micpath.2022.105585

Cited by 11 publications

(9 citation statements)

References 48 publications

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“…Hemolysin, encoded by the hla gene, is one of the most important virulence factors in S. aureus . The hemolysin produced by S.…”

Section: Resultsmentioning

confidence: 99%

“…Hemolysin, encoded by the hla gene, is one of the most important virulence factors in S. aureus. 33 The hemolysin produced by S. aureus can be assessed base on the red blood cell lytic effect using bacterial hemolysis assay. 24,34 Figure 1i shows that CP7 and CP7-FP13-2 significantly inhibited hemolysis of suspended erythrocytes by S. aureus, while FP13-2 and Random-FP13-2 had no such inhibitory effect.…”

Section: Cp7 and Cp7-fp13-2 Inhibit The Expression Of Virulence Factorsmentioning

confidence: 99%

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A Hybrid Antimicrobial Peptide Targeting Staphylococcus aureus with a Dual Function of Inhibiting Quorum Sensing Signaling and an Antibacterial Effect

Lin,

Song,

Zhou

et al. 2023

J. Med. Chem.

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“…Hemolysin, encoded by the hla gene, is one of the most important virulence factors in S. aureus . The hemolysin produced by S.…”

Section: Resultsmentioning

confidence: 99%

Section: Cp7 and Cp7-fp13-2 Inhibit The Expression Of Virulence Factorsmentioning

confidence: 99%

A Hybrid Antimicrobial Peptide Targeting Staphylococcus aureus with a Dual Function of Inhibiting Quorum Sensing Signaling and an Antibacterial Effect

Lin,

Song,

Zhou

et al. 2023

J. Med. Chem.

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“…The red coloration indicates a strong correlation between the residues, and various studies have reported on the diverse activities of catechin and myricitin. Catechin has been reported to exhibit pro-oxidant activity [ 73 ], anti-angiogenic activity in endometriosis [ 74 ], anti-virulence properties [ 75 ], anti-inflammatory effects on human pulp cells [ 76 ], and anti-malarial activity [ 77 ]. Similarly, myricitin has been found to possess antimicrobial activity against food-borne pathogens [ 78 ], antioxidant activity [ 79 ], neurobiological activity [ 80 ], antidiabetic activity [ 81 ], anticancer activity [ 82 ], and analgesic activity [ 83 ].…”

Section: Discussionmentioning

confidence: 99%

In Vitro and In Silico Protocols for the Assessment of Anti-Tick Compounds from Pinus roxburghii against Rhipicephalus (Boophilus) microplus Ticks

Ayub

Malak

Cossío-Bayúgar

et al. 2023

Animals

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Pinus roxburghii, also known by the name “Himalayan chir pine,” belongs to the Pinaceae family. Rhipicephalus (Boophilus) microplus tick is one of the most significant bovine ectoparasites, making it a major vector of economically important tick-borne diseases. The researchers conducted adult immersion tests (AIT) and larval packet tests (LPT) to investigate the acaricidal effect of P. roxburghii plant extract on R. (B.) microplus and its potential modulatory function when used with cypermethrin. Eggs were also assessed for their weight, egg-laying index (IE), hatchability rate, and control rate. After exposure to essential extract concentrations ranging from 2.5 to 40 mg/mL for 48 h, adult female ticks’ oviposition inhibition and unfed R. (B.) microplus larvae’s mortality rates were analyzed. Engorged females exposed to P. roxburghii at 40 mg/mL had reduced biological activity (oviposition, IE) compared to positive and negative controls. A concentration of 40 mg/mL of P. roxburghii caused 90% mortality in R. (B.) microplus larvae, whereas cypermethrin (the positive control) caused 98.3% mortality in LPT. In AIT, cypermethrin inhibited 81% of oviposition, compared to the 40 mg/mL concentration of P. roxburghii, which inhibited 40% of the ticks’ oviposition. Moreover, this study assessed the binding capacity of selected phytocompounds with the targeted protein. Three servers (SWISS-MODEL, RoseTTAFold, and TrRosetta) recreated the target protein RmGABACl’s 3D structure. The modeled 3D structure was validated using the online servers PROCHECK, ERRAT, and Prosa. Molecular docking using Auto Dock VINA predicted the binding mechanisms of 20 drug-like compounds against the target protein. Catechin and myricetin showed significant interactions with active site residues of the target protein, with docking scores of −7.7 kcal/mol and −7.6 kcal/mol, respectively. In conclusion, this study demonstrated the acaricidal activity of P. roxburghii extract, suggesting its potential as an alternative natural acaricide for controlling R. (B.) microplus.

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“…Virulence factors are the main factors involved in the colonization and pathogenicity of pathogenic bacteria and facilitate invasive infection, 36 , 50 , 51 causing a strong inflammatory response and promoting infection syndrome. 4 , 38 , 51 In addition, virulence factors help bacteria form biofilm for stable attachment, ensuring pathogen survival and infection.…”

Section: Discussionmentioning

confidence: 99%

Genetic Diversity, Antibiotic Resistance, and Virulence Gene Features of Methicillin-Resistant Staphylococcus aureus Epidemics in Guiyang, Southwest China

Yang¹,

Wang²,

Li³

et al. 2022

IDR

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Purpose Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most common pathogens of community- and hospital-acquired infections, and its prevalence is increasing globally. Guiyang is the capital city of Guizhou Province, Southwest China; as the transport and tourism centre of Southwest China, Guizhou Province is bordered by Yunnan, Sichuan, Chongqing, and Guangxi Provinces. Although MRSA prevalence is increasing, little is known about its aspects in the area. The purpose of this study was to analyse MRSA molecular characteristics, antimicrobial resistance, and virulence genes in Guiyang. Methods In total, 209 MRSA isolates from four hospitals (2019–2020) were collected and analysed by antimicrobial susceptibility testing and molecular classification by the MLST, spa, and SCC mec typing methods. Isolate antibiotic resistance rates were detected by a drug susceptibility assays. PCR amplification was used to detect the virulence gene-carrying status. Results Twenty-four STs, including 4 new STs (ST7346, ST7347, ST7348, and ST7247) and 3 new allelic mutations, were identified based on MLST. The major prevalent ST type and clone complex were ST59 (49.8%) and CC59 (62.7%), respectively. Spa type t437 (42.1%) and SCC mec IV (55.5%) were identified by spa and SCC mec typing methods as the most important types. Drug sensitivity data showed that the multidrug resistance rate was 79.0%. There were significant differences in multidrug resistance rates and virulence gene-carrying rates for seb, hla, hlb, cna and bap between ST59 and non-ST59 types. Conclusion ST59-SCC mec IV-t437 is a major epidemic clone in Guiyang that should be monitored by local medical and health institutions. The situation differs from other adjacent or middle provinces of China, which may be due to the special geographical location of the region and the trend in antibiotic use or lifestyle. This study provides empirical evidence for local medical and health departments to prevent and control the spread of MRSA.

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Anti-virulence properties of catechin-in-cyclodextrin-in-phospholipid liposome through down-regulation of gene expression in MRSA strains

Cited by 11 publications

References 48 publications

A Hybrid Antimicrobial Peptide Targeting Staphylococcus aureus with a Dual Function of Inhibiting Quorum Sensing Signaling and an Antibacterial Effect

A Hybrid Antimicrobial Peptide Targeting Staphylococcus aureus with a Dual Function of Inhibiting Quorum Sensing Signaling and an Antibacterial Effect

In Vitro and In Silico Protocols for the Assessment of Anti-Tick Compounds from Pinus roxburghii against Rhipicephalus (Boophilus) microplus Ticks

Genetic Diversity, Antibiotic Resistance, and Virulence Gene Features of Methicillin-Resistant Staphylococcus aureus Epidemics in Guiyang, Southwest China

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