Antiamoebic Activity of Petiveria alliacea Leaves and Their Main Component, Isoarborinol

Zavala-Ocampo, Lizeth M.; Aguirre-Hernández, Eva; Pérez‐Hernández, Nury; Rivera, Gildardo; Marchat, Laurence A.; Ramírez‐Moreno, Esther

doi:10.4014/jmb.1705.05003

Cited by 20 publications

(24 citation statements)

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“…The compound of the fractions FB4 and FB5 proved to be the triterpene isoarborinol. This substance is the main compound of the leaves of P. alliacea [ 39 ]. According to NMR spectra, the FB-6 fraction is a mixture of stigmasterol and sitosterol, according to MS and NMR spectra, and also was previously reported in this plant [ 40 ].…”

Section: Resultsmentioning

confidence: 99%

Preferential Activity of Petiveria alliacea Extract on Primary Myeloid Leukemic Blast

Ballesteros-Ramírez

Aldana

Herrera

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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The need for new therapeutic approaches to improve the response in acute leukemia (AL), either by directing therapy or with new therapeutic alternatives, has been a research and clinical interest topic. We evaluated whether blasts from AL patients were sensitive ex vivo to the induction chemotherapy and whether the extracts of Petiveria alliacea (Anamu SC) and Caesalpinia spinosa (P2Et) modulated the sensitivity of leukemic cells to death. Bone marrow samples were taken from 26 patients with de novo AL and 6 in relapse, and the cytotoxicity of the extracts alone or in combination with the chemotherapeutic was evaluated by XTT. Patients were classified as good (GR) and bad responders (BR) according to the ex vivo test. 70.5% of the GR patients to the ex vivo test achieved postinduction remission to induction chemotherapy with a median overall survival of 12.50 months versus 7.23 months in the two groups. Furthermore, it was found that the ex vivo response to extracts and chemotherapeutics is heterogeneous and shows an exclusive pattern between the extracts, Anamu being the more effective in inducing cell death. The combination of extracts with chemotherapeutic agents showed synergistic or antagonistic effects in the patients’ blasts. These results show that the ex vivo evaluation of the sensitivity to induction drugs using primary blasts from patients exhibits a correlation with the response to induction chemotherapy in patients. These analyses would allow establishing a system to predict response to treatment and determine ex vivo susceptibility to new therapies under development, among which is phytotherapeutics.

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Section: Resultsmentioning

confidence: 99%

Preferential Activity of Petiveria alliacea Extract on Primary Myeloid Leukemic Blast

Ballesteros-Ramírez

Aldana

Herrera

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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“…Estudo anterior revelou que o extrato da folha de Petiveria alliacea apresenta atividade antiamoebica que pode ser atribuída a um principal metabólito conhecido como isoarborinol, um triterpeno. Outra caracterização fi toquímica de Petiveria alliacea revelou a presença de triterpenóides, saponinas, polifenois, cumarinas, benzaldeido, ácido benzoico, fl avonoides, fredelinol, pinitol, and allantoina (Zavala-Ocampo et al, 2017).…”

Section: Resultados E Discussõesunclassified

Etnobotânica E O Uso De Plantas Medicinais: Um Relato De Experiência

Fin¹,

Ribeiro²,

Felippe³

et al. 2020

Ciências Agrárias: Conhecimentos Científicos E Técnicos E Difusão De Tecnologias 4

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Todo o conteúdo deste livro está licenciado sob uma Licença de Atribuição Creative Commons. Atribuição 4.0 Internacional (CC BY 4.0).O conteúdo dos artigos e seus dados em sua forma, correção e confiabilidade são de responsabilidade exclusiva dos autores, inclusive não representam a posição oficial da Atena Editora. Permitido o download da obra e o compartilhamento desde que sejam atribuídos créditos aos autores, mas sem a possibilidade de alterá-la de nenhuma forma ou utilizá-la para fins comerciais. Direitos para esta edição cedidos à Atena Editora pelos autores.

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“…Given the aforementioned, the search for new anti-amoebic agents continues to be a global trend, for which different approaches have been taken, from the synthesis of new compounds 29,30 to the systematic study of natural products 14,31 . Anti-amoebic activity is traditionally tested in vitro using axenic strains of E .…”

Section: Discussionmentioning

confidence: 99%

“…For most in vitro studies, axenic cultures of E . histolytica are used 14,15 . However, it has been reported that long-term axenic cultivation has a profound effect on E .…”

Section: Introductionmentioning

confidence: 99%

Development of a Novel Ex-vivo 3D Model to Screen Amoebicidal Activity on Infected Tissue

Guzmán-Delgado

Carranza-Torres

García-Davis

et al. 2019

Sci Rep

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Amoebiasis is a parasitic disease that causes thousands of deaths every year, its adverse effects and resistance to conventional treatments have led to the search of new treatment options, as well as the development of novel screening methods. In this work, we implemented a 3D model of intestine and liver slices from hamsters that were infected ex vivo with virulent E . histolytica trophozoites. Results show preserved histology in both uninfected tissues as well as ulcerations, destruction of the epithelial cells, and inflammatory reaction in intestine slices and formation of micro abscesses, and the presence of amoebae in the sinusoidal spaces and in the interior of central veins in liver slices. The three chemically synthetized compounds T-001, T-011, and T-016, which act as amoebicides in vitro , were active in both infected tissues, as they decreased the number of trophozoites, and provoked death by disintegration of the amoeba, similar to metronidazole. However, compound T-011 induced signs of cytotoxicity to liver slices. Our results suggest that ex vivo cultures of precision-cut intestinal and liver slices represent a reliable 3D approach to evaluate novel amoebicidal compounds, and to simultaneously detect their toxicity, while reducing the number of experimental animals commonly required by other model systems.

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Antiamoebic Activity of Petiveria alliacea Leaves and Their Main Component, Isoarborinol

Cited by 20 publications

References 0 publications

Preferential Activity of Petiveria alliacea Extract on Primary Myeloid Leukemic Blast

Preferential Activity of Petiveria alliacea Extract on Primary Myeloid Leukemic Blast

Etnobotânica E O Uso De Plantas Medicinais: Um Relato De Experiência

Development of a Novel Ex-vivo 3D Model to Screen Amoebicidal Activity on Infected Tissue

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