Antiarthritic Potential of Comprehensively Standardized Extract of Alternanthera bettzickiana: In Vitro and In Vivo Studies

Manan, Maria; Saleem, Uzma; Akash, Muhammad Sajid Hamid; Qasim, Muhammad; Hayat, M; Raza, Zohaib; Ahmad, Bashir

doi:10.1021/acsomega.0c01670

Cited by 42 publications

(27 citation statements)

References 69 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This augmented level of PGE 2 accelerates the differentiations of osteoclast and generating the degrading enzymes and hence stimulates the bone erosion, vasodilation and migration of leukocytes at site of in ammation. So, any substance that prevents the generations of aforementioned mediators carries great importance in the management of rheumatism (Manan et al, 2020). In current study, increased expression of COX-2 and PGE-2 were noted in diseased controlled rats, however, signi cant decrease in COX-2 and prostaglandin-E2 were observed in rats treated with nerolidol.…”

Section: Discussioncontrasting

confidence: 44%

Nerolidol: a potential approach in rheumatoid arthritis through reduction of TNF-α, IL-1β, IL-6, NF-kB, COX-2 and antioxidant effect in CFA-induced arthritic model

et al. 2022

View full text Add to dashboard Cite

Rheumatoid arthritis an autoimmune infectious disorder, is categorized by in ammation and increased level of pro-in ammatory cytokines which are released by immune cells, macrophages or activation of arachidonic acid metabolism. The expression of these cytokines, oxidative free radicals and the activation of COX-2 enzymes are crucial targets for chronic in ammation. On the basis of established anti-in ammatory e cacy of Nerolidol, the primary study was further appraised to determine its e cacy against Freund's complete adjuvant (CFA) rheumatoid model. Arthritis was persuaded by inoculation of 0.1mL CFA injection into left hind footpad of rats. Anti-arthritic potential of nerolidol (at 200, 400 and 800mg/kg doses) was assessed by measuring the paw volume, body weight, serum analysis, histopathological and radio-graphics of ankle joints. Expressions of cytokine's panels like IL-10, IL-4, COX-2, NF-K β, TNF-α, IL-6, PGE-2 and IL-1β were determined by real time qPCR. Antioxidant enzyme analyses was calculated by measuring the SOD, POD and catalase activity from serum and equated with arthritic control group. Nerolidol prevented the body weight loss, stabilized the biochemical and haematological homeostasis and signi cantly reduced the paw volume. Furthermore, X-ray and histopathological assessment of ankle joints showed an improvement in the joint structure of rats treated with nerolidol. Besides that, over expression of gene pointers like TNF-α, IL-1β, IL-6, NF-K β, PGE-2 and COX-2 in CFA treated control rats were also reversed with nerolidol. This antiarthritic mechanism was further supported by the increased level of IL-10, IL-4 and serum anti-oxidant activity. The present ndings demonstrate that nerolidol reduce the adjuvant arthritis by down-regulating the proin ammatory cytokines and up-regulating the aforementioned anti-in ammatory cytokines and may be used as a therapeutic substance for the management of human rheumatoid arthritis.

show abstract

Section: Discussioncontrasting

confidence: 44%

Nerolidol: a potential approach in rheumatoid arthritis through reduction of TNF-α, IL-1β, IL-6, NF-kB, COX-2 and antioxidant effect in CFA-induced arthritic model

et al. 2022

View full text Add to dashboard Cite

show abstract

“…They have found that even the 250 mg/kg/day of the ethanolic extract was able to modulate the parameters suggesting the antiarthritic potential when compared with standard drug and disease control. In silico analysis suggested the strong interaction between the HPLC-analysed phytomolecules and cyclooxygenases ( Manan et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

The Genus Alternanthera: Phytochemical and Ethnopharmacological Perspectives

et al. 2022

View full text Add to dashboard Cite

Ethnopharmacological relevance: The genus Alternanthera (Amaranthaceae) comprises 139 species including 14 species used traditionally for the treatment of various ailments such as hypertension, pain, inflammation, diabetes, cancer, microbial and mental disorders.Aim of the review: To search research gaps through critical assessment of pharmacological activities not performed to validate traditional claims of various species of Alternanthera. This review will aid natural product researchers in identifying Alternanthera species with therapeutic potential for future investigation.Materials and methods: Scattered raw data on ethnopharmacological, morphological, phytochemical, pharmacological, toxicological, and clinical studies of various species of the genus Alternanthera have been compiled utilizing search engines like SciFinder, Google Scholar, PubMed, Science Direct, and Open J-Gate for 100 years up to April 2021.Results: Few species of Alternanthera genus have been exhaustively investigated phytochemically, and about 129 chemical constituents related to different classes such as flavonoids, steroids, saponins, alkaloids, triterpenoids, glycosides, and phenolic compounds have been isolated from 9 species. Anticancer, antioxidant, antibacterial, CNS depressive, antidiabetic, analgesic, anti-inflammatory, and immunomodulator effects have been explored in the twelve species of the genus. A toxicity study has been conducted on 3 species and a clinical study on 2 species.Conclusions: The available literature on pharmacological studies of Alternanthera species reveals that few species have been selected based on ethnobotanical surveys for scientific validation of their traditional claims. But most of these studies have been conducted on uncharacterized and non-standardized crude extracts. A roadmap of research needs to be developed for the isolation of new bioactive compounds from Alternanthera species, which can emerge out as clinically potential medicines.

show abstract

“…The quercetin inhibits CNS and spinal cord glial cells activation, and consequently reduces cytokine production and oxidative stress resulting in reduced neuro-in ammation and hyperalgesia [30]. Catechin, belonging to the avonoid family, can alleviate the in ammation and pain response in rats by decreasing the generation of TNF-α, PGE2, and IL-1β [31].We now know that circulating matrix metalloprotease 9 (MMP-9) plays an important role in neuro-in ammation and oxidative stress as two leadings factors of the neuropathic pain. It has been proved that Catechin reduces MMP-9 in diabetic rats [1].…”

Section: Discussionmentioning

confidence: 99%

Protective Effect of Cichorium Intybus Root Extract and Esculetin on Lipopolysaccharide-Induced Pain and Inflammation in Male Mice: A Potential Role for Tumor Necrosis Factor-α and Interleukin-1β

Taleghani

Rasouli

Rostampour

et al. 2022

Preprint

View full text Add to dashboard Cite

Lipopolysaccharide (LPS) has been widely used in animal modeling of inflammation-induced hyperalgesia. Proinflammatory cytokines (TNF-α, IL-1β) are involved in the pathogenesis of pain. Cichorium intybus (CI) contains many compounds which inhibit inflammatory mediators. The present study was designed to evaluate the effect of CI root hydro-alcoholic extract (CE) and Esculetin (ESC) - a bioactive coumarin compound of CI- on LPS-induced pain and inflammation in male mice. CE constituents were evaluated by GC-MS. Two different concentration of CE (300 and 500 mg/kg/p.o.) and ESC (40 mg/kg/i.p.,o.) were used for experiments. Drugs were administered once daily for 7 days. On day 7, LPS (0.83 mg/kg/i.p.) was injected 30 min after the last drug administration. Blood samples were collected 90 min after LPS injection. Hot plate and formalin tests were performed 24 hours after LPS injection. Serum TNF-α and IL-1β levels were measured using ELISA. LPS significantly increased the response time of animals in hot plate and both phases of formalin tests. CE (300 and 500 mg/kg/p.o.) significantly reduced the response time in comparison with the LPS group in hot plate and both phases of formalin tests. CE (500 mg/kg/p.o.) reduced serum TNF-α and IL-1β levels. Esculetin increased the response time in hot plate and the second phase of formalin test compared to the LPS group and also decreased IL-1β level, significantly. Our results demonstrated that CE exhibited anti-nociceptive and anti-inflammatory effects better than esculetin. The probable mechanism of anti-hyperalgesic effect of CE is related to inhibition of inflammatory cytokines IL-1β and TNF-α.

show abstract

Antiarthritic Potential of Comprehensively Standardized Extract of Alternanthera bettzickiana: In Vitro and In Vivo Studies

Cited by 42 publications

References 69 publications

Nerolidol: a potential approach in rheumatoid arthritis through reduction of TNF-α, IL-1β, IL-6, NF-kB, COX-2 and antioxidant effect in CFA-induced arthritic model

Nerolidol: a potential approach in rheumatoid arthritis through reduction of TNF-α, IL-1β, IL-6, NF-kB, COX-2 and antioxidant effect in CFA-induced arthritic model

The Genus Alternanthera: Phytochemical and Ethnopharmacological Perspectives

Protective Effect of Cichorium Intybus Root Extract and Esculetin on Lipopolysaccharide-Induced Pain and Inflammation in Male Mice: A Potential Role for Tumor Necrosis Factor-α and Interleukin-1β

Contact Info

Product

Resources

About