Antibacterial Activities of Kappa-Carrageenan Oligosaccharides

Abstract

Κ-carrageenan oligosaccharides were prepared by enzymatic degradation. The inhibiting activities of the prepared κ-carrageenan oligosaccharides against Escherichia coli (E.coli ), Staphylococcus aureus (S.aureus), Saccharomyces cerevisiae (S.cere), Penicillium citrinum (P.citr) and Mucor.sp were determined and compared by measuring the inhibitory zone diameter. The results indicate that all tested κ-carrageenan oligosaccharides have inhibiting activity against the five bacteria, but the inhibiting activities against Saccharomyces cerevisiae is higher than against any other four bacteria. The inhibiting concentration (MIC) of κ-carrageenan oligosaccharides are 1mg/ml, 5mg/ml, 20mg/ml, and antibacterial activity of κ-carrageenan oligosaccharides was inversely affected by concentration, with the highest activity at the concentration of 5mg/ml.

Antibacterial Activity of Polyethylenimine/Carrageenan Multilayer against Pathogenic Bacteria

“…Kappa carrageenan and lambda carrageenan showed an increase of growth in E. cloaceae by 18.0% and 17.44% respectively (Figure 7). Although the results of bacterial growth inhibition obtained from carrageenan were not that quite high for S. aureus, it indeed showed that it has antibacterial activity as previously reported [20]. Figure 8, Figure 9 showed the paper strip test for combined action of double layer and multilayers of carrageenan and PEI.…”

“…In one study, oligosaccharides from κ-carrageenan have antibacterial activities against E. coli, S. aureus, S. cere, P. citr and Mucor sp. [20].…”

Evaluation of cytotoxicity and antimicrobial activity of an injectable bone substitute of carrageenan and nano hydroxyapatite

“…Therefore, depending on their molecular weight, several of the CG molecular chains were comprised of two or more monosaccharides that could link to glycoproteins and are associated with the outer side of the bacterial cytoplasmic membrane, especially in Gram‐negative bacteria . It has been reported by Wang et al that short chains of CG have antimicrobial activity. According to their research findings, however, the mechanism by which cell death occurs is not well known.…”

“…According to their research findings, however, the mechanism by which cell death occurs is not well known. Potentially, the union of these chains to the surface of the bacteria produces structural changes in the membrane, increasing the permeability and leading to bacterial death …”

Carrageenan: Drug Delivery Systems and Other Biomedical Applications

“…Another way to evaluate the antibacterial efficacy of carrageenans was through the use of kappa -CG oligosaccharides against Escherichia coli , Staphylococcus aureus , Saccharomyces cerevisiae , Penicillium citrinum , and Mucor spp., determining the antibacterial efficacy by measuring the diameter of the inhibitory zone. Consequently, it was observed that all kappa -CG oligosaccharides had inhibitory activity against the bacteria studied, presenting a greater inhibitory activity against Saccharomyces cerevisiae [ 78 ].…”