Rose myrtle, or (Rhodomyrtus tomentosa [Ait.] Hassk), is a plant that belongs to the Myrtaceae family. The rose myrtle plant is one of the biodiversity that must be developed sustainably because of its properties as an antidiabetic, diarrhea, burns, bleeding wounds, and stomachaches. Rose myrtle leaves contain rhodomyrtone antibiotic compounds. The rhodomyrtone compound belongs to the phloroglucinol derivatives and active against Escherichia coli and Staphylococcus aureus [5]. Rose myrtle leaves have been used by society to treat various diseases related to bacterial infections, such as dysentery and typhoid fever caused by S. dysenteriae and Salmonella Typhi. The antibacterial test results showed that n-hexane and ethyl acetate extracts were active against both bacteria, while ethanol extract was not. Isolates N1 and E1 were produced, respectively, from n-hexane extract and ethyl acetate extract. The MIC values of both N1 and E1 for S. dysenteriae and S. Typhi were the same, namely, 125 μg/mL. Isolate N1 was an essential oil containing menthol (59.60%), caryophyllene (25.77%), and cubenol (14.63%), while isolate E1 was essential oil containing (73.93%), pentanone (8.30%), alpha calacorene (7.58%), and calacorene (3.78%) [6].The application of rose myrtle leaves as traditional medicine needs to be proven for its efficacy and safety. Fraction of n-hexane and ethyl acetate of rose myrtle leaves showed antibacterial activity against Streptococcus mutans (MIC of each fraction was 7.8 g/mL