Antibacterial activity of lysozyme-loaded cream against MRSA and promotion of scalded wound healing

Chen, Liang-Liang; Shi, Wen-Pu; Zhang, Tuo-Di; Zhou, Ya-Qing; Zhao, Feng-Zhu; Ge, Wan-Yi; Jin, Xiao-Qian; Lin, Wilson; Guo, Weihong; Yin, Da‐Chuan

doi:10.1016/j.ijpharm.2022.122200

Cited by 18 publications

(12 citation statements)

References 77 publications

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“…Our result was also similar to it of wound treated with lysozyme separated from EW. The wound was almost healed (13.36%) after lysozyme treatment on the 10th day [47]. In addition, we found that the mixture with a medium dose of sericin (0.2 g ml −1 ) showed the best repair effect.…”

Section: Discussionmentioning

confidence: 70%

Effects of sericin and egg white on the inflammation of damaged skin in mice

Dong

Zuo

et al. 2023

Biomed. Mater.

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Background: Sericin and egg white (EW) have shown the ability to promote wound healing. However, there have been insufficient studies regarding the effects of sericin and EW mixtures on wound healing. This study aimed to investigate the effects of a hybrid sericin and EW solution on wound repair and inflammation-related indicators in mouse skin. Methods: In this work, sericin with a low molecular weight was first mixed with homogeneous EW to prepare a hybrid wound dressing. Histology evaluation, the expression of C-reactive protein (CRP) and inflammatory cytokines in mice were tested to determine the effects of this dressing on skin injuries in mice. Results: The results showed that sericin and the hybrid solution of sericin and EW effectively promoted wound healing in mouse skin. The wound recovery rates of mice 12 days after treatment with a medium dose of sericin (0.2 g/mL) and the same dosage of sericin with added EW were 1.32 and 1.65 times that of mice treated with phosphate buffer saline (PBS) as a control, respectively. In addition, the mixture solution was more effective in wound healing than sericin alone. Sericin with EW significantly reduced the expression of CRP and inflammatory cytokines in mice during wound healing. Conclusion: A sericin and EW hybrid solution can effectively shorten the time needed for wound healing and reduce inflammation-related indicators in mice, making it a promising candidate for wound dressing.

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Section: Discussionmentioning

confidence: 70%

Effects of sericin and egg white on the inflammation of damaged skin in mice

Dong

Zuo

et al. 2023

Biomed. Mater.

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“…HT22 and HeLa cells were used to evaluate cytotoxicity. The acute skin toxicity, irritation, and sensitization of the cream were evaluated based on methods previously published in the literature . Briefly, Sprague-Dawley (SD) rats (male, 150–180 g) were depilated on both sides of the dorsal spine, and LA-6 was applied to the left area.…”

Section: Methodsmentioning

confidence: 99%

“…LYZ was prepared from hen egg whites via membrane separation and crystallization techniques. 28 Briefly, fresh hen egg whites were diluted with NaCl solution (100 mM), separated by ultrafiltration at a cutoff of 20 kDa, concentrated, and crystallized by adding NaCl (30 mg/mL) at 4 °C. The crystals were collected by centrifugation and dialyzed, and LYZ was obtained after freeze-drying.…”

Section: Methodsmentioning

confidence: 99%

“…The acute skin toxicity, irritation, and sensitization of the cream were evaluated based on methods previously published in the literature. 28 Briefly, Sprague-Dawley (SD) rats (male, 150−180 g) were depilated on both sides of the dorsal spine, and LA-6 was applied to the left area. In the acute skin toxicity and irritation groups, the right side was rubbed with coarse sandpaper until the skin oozed blood and then LA-6 was applied.…”

Section: Determination Of the Inhibition Zonementioning

confidence: 99%

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Synthesis of Silver Nanoparticles Using Lysozyme for Scald-Wound Healing

Chen,

Shi,

Zhang

et al. 2023

ACS Appl. Nano Mater.

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The use of natural proteins is a green and effective method for synthesizing silver nanoparticles (AgNPs). Aromatic amino acids of proteins play a reducing role in synthesis, and proteins are coated on the surface of AgNPs to play the role of stabilizers. In this study, we developed an efficient one-step method for synthesizing AgNPs using lysozyme (LYZ) under visible-light irradiation from a xenon lamp in situ. The light conditions and LYZ structure could substantially affect the synthesis. Antibacterial and scald-wound healing results showed that LYZ/AgNPs had broadspectrum and efficient antibacterial activity and promoted scaldwound healing. Therefore, the proposed synthesis method and LYZ/AgNPs have good practical application prospects.

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“…LYS is widely used in the food industry, including kimchi pickling, sushi, Chinese noodles, and cheese [ 77 , 78 , 79 ]. In the pharmacological field, LYS has extensive use in wound-healing creams, eye drops, anti-cancer drugs, and as an antiviral agent and immunomodulatory [ 80 , 81 , 82 , 83 ]. Recent studies have shown that a non-peptide fragment (HL9) from human LYS has anti-HIV-1 activity at nanomolar concentrations [ 84 , 85 ].…”

Section: Fractionation Of Egg Proteinsmentioning

confidence: 99%

The Impact of Processing and Extraction Methods on the Allergenicity of Targeted Protein Quantification as Well as Bioactive Peptides Derived from Egg

et al. 2023

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This review article discusses advanced extraction methods to enhance the functionality of egg-derived peptides while reducing their allergenicity. While eggs are considered a nutrient-dense food, some proteins can cause allergic reactions in susceptible individuals. Therefore, various methods have been developed to reduce the allergenicity of egg-derived proteins, such as enzymatic hydrolysis, heat treatment, and glycosylation. In addition to reducing allergenicity, advanced extraction methods can enhance the functionality of egg-derived peptides. Techniques such as membrane separation, chromatography, and electrodialysis can isolate and purify specific egg-derived peptides with desired functional properties, improving their bioactivity. Further, enzymatic hydrolysis can also break down polypeptide sequences and produce bioactive peptides with various health benefits. While liquid chromatography is the most commonly used method to obtain individual proteins for developing novel food products, several challenges are associated with optimizing extraction conditions to maximize functionality and allergenicity reduction. The article also highlights the challenges and future perspectives, including optimizing extraction conditions to maximize functionality and allergenicity reduction. The review concludes by highlighting the potential for future research in this area to improve the safety and efficacy of egg-derived peptides more broadly.

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Antibacterial activity of lysozyme-loaded cream against MRSA and promotion of scalded wound healing

Cited by 18 publications

References 77 publications

Effects of sericin and egg white on the inflammation of damaged skin in mice

Effects of sericin and egg white on the inflammation of damaged skin in mice

Synthesis of Silver Nanoparticles Using Lysozyme for Scald-Wound Healing

The Impact of Processing and Extraction Methods on the Allergenicity of Targeted Protein Quantification as Well as Bioactive Peptides Derived from Egg

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