Antibacterial Activity of Tanacetum vulgare L. Extracts against Clinical Isolates of Bovine Mastitis

Abstract

A bovine mastitis is an infectious disease, which is usually treated with antibiotics. Alternatively, herbal medicine has been proposed due to bacterial resistance. The aim of this study was to determine the antibacterial activity of the acetonic and ethanolic extracts of dried flowers and leaves of Tanacetum vulgare L. against bovine mastitis-inducing clinical isolates such as Escherichia coli, Streptococcus agalactiae, Streptococcus uberis, Serratia liquefaciens, Staphylococcus aureus, and reference cultures of S. aureus and E. coli. The extracts of T. vulgare showed partial antibacterial activity against tested strains of S. aureus. The MIC and MBC values of a 70% ethanol extract of flowers (MIC = 3.4 mg/mL, MBC = 3.4–6.8 mg/mL) were lower than for the 70% ethanol extract of leaves (MIC = 15.7–31.4 mg/mL, MBC = 62.9–125.9 mg/mL). The flower extracts showed low activity against E. coli (MIC = 53.9 mg/mL, MBC = 53.9–107.8 mg/mL) and S. agalactiae (MIC, MBC = 53.9 mg/mL). T. vulgare leaf extracts had minimal antibacterial effects against Streptococcus strains (MIC = 31.4–62.9 mg/mL, MBC = 53.9–125.9 mg/mL) and Serratia liquefaciens (MIC, MBC = 125.9 mg/mL). However, flower extracts had a higher phenolic content that did not correlate with antibacterial effects. T. vulgare flower and leaf extracts could be combined to obtain broader antibacterial effects.

Chemical Profiling and Antioxidant Activity of Tanacetum vulgare L. Wild-Growing in Latvia

“…The biological and pharmacological assays are reported to strongly depend not only on the morphological part of T. vulgare but also on the extraction solvents used [ 4 , 18 , 20 ]. Generally, hydroalcoholic extracts and infusions could be considered more active compared to lipophilic n-hexane extracts.…”

Anthelmintic Activity of Tanacetum vulgare L. (Leaf and Flower) Extracts against Trichostrongylidae Nematodes in Sheep In Vitro

“…No other studies were found that used acetone as the solvent for T.vulgare extract. In our study, we used this solvent and there is a study that compared total phenolic compounds in ethanol and acetone extracts; acetone extracts contain more of these components than ethanol [ 52 ]. If compare the types of extract used in our study with this study, the acetone 30% flower extract contains the most phenolics.…”

“…If compare the types of extract used in our study with this study, the acetone 30% flower extract contains the most phenolics. It was the only extract that had no antibacterial effect [ 52 ]. This was also proven in our research as this extract showed weaker ovicidal and larvicidal activity.…”

Antibacterial Activity of Tylophora tenuis Methanol Extract on Staphylococcus aureus

“…Previous studies show the high MIC and MBC value of crude extract in antibacterial activity. The antibacterial activity of Tanacetum vulgare crude extract tested on E. coli, S. agalactiae, T. vulgare, Streptococci, and Serratia liquefaciens is range from 53.9 to 125.9 mg/mL (19) or the antibacterial ability of Bixa orellana stem and leaves extract on B. cereus were with MIC = 34.71 and 14.11 mg/mL, respectively (20). Therefore, T. tenuis has the potential to be an antibacterial candidate from nature.…”