Antibacterial activity of the sambiloto leaf extracts (Andrographis paniculata) to Bacillus cereus and Pseudomonas aeruginosa

Abstract

Mardiana RN, Handayani N. 2016. Antibacterial activity of the sambiloto leaf extracts (Andrographis paniculata) to Bacillus cereus and Pseudomonas aeruginosa. Biofarmasi 14: 19-24. The purpose of this research was to evaluate the antibacterial activity of sambiloto ( Andrographis paniculata Nees. ) extract against bacterial Bacillus cereus and Pseudomonas aeruginosa. Some research had proven sambiloto leaf extract evident had antibacterial activity to bacteria Staphylococcus aureus. The sample of sambiloto leaf was macerated using 70% ethanol, and the extract was concentrated using rotary evaporator. The antibacterial activity was evaluated by diffusion method. Antibacterial potency of the extract was compared with by Amoxicillin made default curve among logarithm concentrates to constraining diameter (mm), then accounted by its equivalence point. Ethanol extract of sambiloto leaf had antibacterial activity to B. cereus and P. aeruginosa. Minimum Inhibitory Concentration (MIC) of 12,5% against B. cereus and P. aeruginosa. The potential antibacterial activity of sambiloto leaf extract was 0,2% for B. cereus and 0.3% for P. aeruginosa; as compared with Amoxicillin. This, potential antibacterial activity of sambiloto leaf extract to the two tested bacteria was much smaller than that of Amoxicillin synthetic antibiotic.

Potential of Sambiloto (Andrographis paniculata Nees) Extract against Salmonella Pullorum

“…The obtained liquid extract from then were filtered through filter paper (Ousaaid et al, 2020). The macerate was evaporated with a rotary evaporator until no solvent was left behind and produced sambiloto extract (Mardiana and Handayani, 2016). Then, sambiloto extract was diluted with distilled water to obtain 4 tubes of sambiloto extract with concentrations of 5%, 10%, 20% and 40%.…”

Perbandingan Antibakteri Ekstrak Daun Sambiloto (Andrographis paniculata) dan Daun Kemangi (Ocimum sanctum) terhadap Pertumbuhan Bakteri Staphylococcus aureus

“…Saat ini, pemakaian bahan alam sebagai obat tradisional dan olahannya mengalami perkembangan pesat dengan banyaknya peminat, karena bahan alami tersebut dapat mengobati berbagai macam penyakit seperti antiinflamasi, antipiretik, analgesik dan ada juga yang memiliki sifat bakteriostatik yaitu dapat menghambat pertumbuhan bakteri tertentu, serta efek samping yang ditimbulkan dari obat tradisional sangatlah kecil (Mardiana & Handayani, 2017).…”

“…Semua filtrat yang didapatkan diuapkan atau dipekatkan dengan rotary vacuum evaporator pada suhu 40°C hingga diperoleh ekstrak, kemudian ekstrak dikentalkan dengan menggunakan waterbath hingga menjadi ekstrak kental daun sambiloto (Andrographis paniculata) dan daun kemangi (Ocimum sanctum). Selanjutnya pembuatan variasi konsentrasi ekstrak daun sambiloto (Andrographis paniculata) dan daun kemangi (Ocimum sanctum) sebagai larutan uji menggunakan beberapa konsentrasi yaitu 6,25% hingga 100% dengan pengenceran menggunakan pelarut aquadest (Mardiana & Handayani, 2017).…”

PENGARUH PEMANASAN, TINGKAT KEASAMAN (pH) DAN KONSENTRASI GARAM TERHADAP STABILITAS ANTI BAKTERI EKSTRAK ETANOL KULIT KAYU AKWAY (Drimys piperita Hook f.)

“…Pengujian daya hambat ekstrak etanol kulit kayu akway dilakukan pada konsentrasi 0- (Singh et al, 2017), daun Andrographis paniculata (Mardiana & Handayani, 2016) dan daun Quercus infectoria (Shariatifar et al, 2014).…”