Dayak onion (Eleutherine palmifolia (L.) Merr.) is one plant that can cure various diseases and symptoms such as constipation, dysuria, cancer, dysentery, ulcers, diabetes mellitus, hypertension, antihypercholesterol, antimelanogenesis and antioxidant. However, there is no report about the chemical composition and their isolation. The purpose of this research is to extract and isolate the alkaloid compounds from Dayak onion and to identify the alkaloids by UV and FTIR spectrophotometer.TLC of alkaloid extracts from the plants used in this study revealed the presence of these compounds by using Bouchardat's reagent to reveal characteristic orange bands of alkaloids. From the data obtained, The isolation of the crude alkaloid extract obtained two pure isolates, they are isolated A (Rf=0.51) and isolate B (Rf=0.57). Isolate A gives a maximum absorbance at 270 nm wavelength and isolate B at 271 nm. The results of FTIR showed the presence of aliphatic CH, CH 2 , C=O, C=C, C-N, C-O for isolate A and aliphatic CH, CH 2 , CH 3 , C=C, C-N, C-O for isolate B.Based on the above results, it can be concluded that Dayak onion has a potential of alkaloids constituents for further study.