Antibacterial Activity Tests of Staphylococcus Aureus and Phytochemical Screening in Family Asteraceae, Clusiaceae, Phyllanthaceae

Abstract

Infection is a type of disease that affects many residents of developing countries, including Indonesia. One pathogenic bacteria that is quite dangerous and causes skin infections both sporadically and endemically is S aureus. Several studies intensively report that some of the Asteraceae, Clusiaceae and Phyllanthaceae family plants contain alkaloids, flavonoids, saponins and tannins that have potential as antibacterial S aureus. Therefore, this study aims to identify effective plants to deal with infections caused by S aureus bacteria. To this end, the ingredients used are the leaves of G. procumbens, E. scaber, G. mangostana, G atroviridis, G. xanthochymus, leaves and bark of A. neurocarpum. Plant material extraction was done by maceration using 90% ethanol solvent. Next, an antibacterial test was conducted, which was begun by testing the Minimum Inhibitory Concentration followed by testing the Obstacle Area Width. The Minimum Inhibitory Concentration test was carried out using the agar dilution method, and the Obstacle Area Width test was carried out with paper diffusion discs. The results showed that all the plants have potential as antibacterials, and the mangosteen leaf extract has the largest Obstacle Area Width of 5.1 mm with a concentration of extract of 30% and a fairly strong inhibitory ability.

Research Progress on Antibacterial Activities and Mechanisms of Natural Alkaloids: A Review

“…The antibacterial activity of alkaloids is related to many factors, such as the content of active substances in the extract, which are affected by internal and external factors. Internal factors are affected by plant genetics, whereas external factors are more affected by plant physiology and ecology [114]. The bioavailability of alkaloids is also a widely shared concern.…”

Antibacterial Activities Of Papaya Seed (Carica papaya L.) Ethanol Extract With MAE And UAE Extraction Methods Towards Staphylococcus aureus

“…The results of the antibacterial activity test of papaya seed ethanol extract can be seen in Figure 2. Antibacterial activity test of papaya seed ethanol extract (EEBP) using the MAE and UAE extraction methods against Staphylococcus aureus bacteria showed the presence of Radical Inhibitory Power (DDH) which was characterized by a clear area around the paper disc that was not overgrown with bacteria (Djarot et al, 2020). The ethanol extract maceration method of papaya seeds has been shown to have antibacterial activity against Staphylococcus aureus, it ranged 6,00-7,00 mm (Torar et al, 2017).…”

Antibacterial and antibiotic potentiating capabilities of extracts isolated from Burkillanthus malaccensis, Diospyros hasseltii and Cleisthanthus bracteosus against human pathogenic bacteria

“…Plant selection was done based on the families of these plants (Phyllanthaceae, Rutaceae and Ebenaceae) being attributed with antibacterial capabilities in previous studies (Khulbe & Sati, 2009;Ngane, 2019;Nematollahi et al, 2011;Bitchagno et al, 2015;Djarot et al, 2020). The plants were collected around the villages in the Manong district in Northern Perak.…”