Antibacterial and NMR analysis of Curcuma xanthorrhiza extract and fractions

Diastuti, Hartiwi; Syah, Yana Maolana; Juliawaty, Lia Dewi; Wibowo, Marlia Singgih

doi:10.5614/j.math.fund.sci.2014.46.3.2

Cited by 13 publications

(9 citation statements)

References 31 publications

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“…The extract showed inactive against Escherichia coli, Enterobacter aerogenes and Salmonella thypi, while the chloroform fraction was devoid of activities (Diastuti, Syah, Juliawaty, & Singgih, 2014). Xanthorrhizol isolated from the ethanol extract of Curcuma xanthorrhiza is a sesquiterpene compound showed strongly antibacterial activity with thermal and pH stability (Lee, Shim, Rukayadi, & Hwang, 2008).…”

Section: Resultsmentioning

confidence: 99%

Characterization of Curcuminoid from Curcuma xanthorrhiza and Its Activity Test as Antioxidant and Antibacterial

Atun

Aznam

Arianingrum

et al. 2020

Molekul

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Curcuminoids are the main component found in many Zingiberaceae family plants. The aim of this study was to characterize curcuminoid and its activity test as an antioxidant and antibacterial. Dryed powder of C. xanthorrhiza (1 kg) was macerated with ethanol for 24 hours at room temperature. Ethanol extract of C. xanthorrhiza was subsequently fractionated with n-hexane and chloroform to take the yellow or orange indicated contain of curcuminoids. Analysis of total phenolic levels was carried out by the Follin-Ciaocalteau method. The isolation of curcuminoid componens from this fraction was carried out by chromatographic method and the structure elucidation was performed by interpretation of spectroscopic data, including UV, IR, 1H and 13C NMR 1D and 2D. The antioxidant activity test used the DPPH (2,2-diphenylpicrylhydrazyl) method, while the antibacterial activity test used Kirby Bauer test diffusion method. The results showed that the curcuminoid fraction yield was 10.06% from ethanol extract C. xanthorrhiza. The total phenolic content of curcuminoids fraction was 745.45 ± 18.5 mg galic acid (GA)/g extract. Curcuminoids fraction was isolated a known compound desmethoxycurcumin (1). The content of demethoxycurcumin (1) in curcuminoid fraction is 20.97 %.The antioxidant activity of curcuminoids fraction showed strongest activity with IC50 24.98 µg/ml. Antibacterial activity against of the four pathogenic bacteria showed medium activity. The study suggests that curcuminoids extract from C. xanthorrhiza rhizome have potential compounds could be suitable for antioxidant and the treatment of various infections caused of microbial.

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Section: Resultsmentioning

confidence: 99%

Characterization of Curcuminoid from Curcuma xanthorrhiza and Its Activity Test as Antioxidant and Antibacterial

Atun

Aznam

Arianingrum

et al. 2020

Molekul

View full text Add to dashboard Cite

show abstract

“…Antibiotic resistance levels are rising, therefore the search for novel bactericidal agents has increased over the past years. A main focus is using medicinal herbs that are widely available, have fewer side effects and have excellent antimicrobial potential [28]. Hence, the investigation of substitute medicines from plants that are active against multi-resistant pathogenic bacteria has increased all around the globe [29].…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial, Antioxidant and Cytotoxic Potential of Aerial Parts of Monotheca buxifolia

et al. 2019

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In this study, different aerial parts of Monotheca Buxifolia were screened for their antibacterial, antifungal, and anti-oxidant activity. Extract of the fruit was most active against P. mirabilis, MSSA and M. morganii strains. Moreover, the methanolic fruit extract demonstrated good antibacterial properties against VRSA, S. aureus and Acinetobacter. Despite having good antibacterial properties, Monotheca buxifolia showed low antifungal activity. The cytotoxic activity was the highest in the pet-ether fruit extract, causing 60% of lethality against Artemia salina larvae with LD 50 at 3.307. The highest scavenging activity was shown by the methanolic leaf extract in a concentrationdependent pattern. The findings of this study suggest that Monotheca Buxifolia has good antibacterial, antioxidant potential but low antifungal properties.

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“…The results indicated that a high concentration of the extract was needed to inhibit and kill the P. aeruginosa culture (200 and 800 mg/mL, respectively). In the study done by Diastuti et al 50 , the MIC and MBC values of the C. xanthorrhiza Roxb. extracts on the P. aeruginosa were much lower than in this study.…”

Section: Determination Of Minimum Inhibitory Concentration (Mic) and mentioning

confidence: 99%

Inhibition of Pseudomonas aeruginosa Quorum Sensing by Curcuma xanthorrhiza Roxb. Extract

Othman¹,

Rukayadi²,

Radu³

2019

J Pure Appl Microbiol

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Microorganisms such as Pseudomonas aeruginosa have always been adaptable in surviving the harsh environment such as antimicrobial agents via the quorum sensing (QS) mechanism. Studies have shown that quorum sensing mechanism cases have been highly associated with foodborne illnesses. Since synthetic compounds such as azithromycin (AZM) are reported to have detrimental effects on human, using medicinal local plants have been gaining attention as an anti-quorum agent. The aim of this study was to determine the anti-quorum sensing activity of the Curcuma xanthorrhiza Roxb. extract against P. aeruginosa ATCC35554 quorum sensing system including swarming motility, pyocyanin production and biofilm formation. The results indicated that the extract required a high concentration to inhibit and kill the P. aeruginosa with minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC) values of 200 and >700 mg/mL, respectively. Thus, anti-quorum sensing assays were done in concentration up to 200 mg/mL. The inhibition of quorum sensing activity of C. xanthorrhiza Roxb. extract on P. aeruginosa quorum sensing was concentration dependent manner. At 200 mg/mL of the extract exhibited 72.12% reduction of swarming motility, 84.30% inhibition of the pyocyanin production and 78.35% reduction in the biofilm formation. In conclusion the crude extract of C. xanthorrhiza Roxb. extract has ability to reduce the virulence factors; swarming motility, pyocyanin production and biofilm formation regulated by quorum sensing. Thus, the extract C. xanthorrhiza Roxb. extract has anti-quorum sensing or quorum quenching activity.

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Antibacterial and NMR analysis of Curcuma xanthorrhiza extract and fractions

Cited by 13 publications

References 31 publications

Characterization of Curcuminoid from Curcuma xanthorrhiza and Its Activity Test as Antioxidant and Antibacterial

Characterization of Curcuminoid from Curcuma xanthorrhiza and Its Activity Test as Antioxidant and Antibacterial

Antimicrobial, Antioxidant and Cytotoxic Potential of Aerial Parts of Monotheca buxifolia

Inhibition of Pseudomonas aeruginosa Quorum Sensing by Curcuma xanthorrhiza Roxb. Extract

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