Antibacterial effect of gold nanoparticles against <i>Corynebacterium pseudotuberculosis</i>

Mohamed, Marwah; Fouad, Shereen A.; Elshoky, Hisham A.; Mohammed, Gina M.; Salaheldin, Taher A.

doi:10.1016/j.ijvsm.2017.02.003

Cited by 173 publications

(84 citation statements)

References 20 publications

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“…The antibacterial activity of green synthesized gold nanoparticles against gram positive and gram negative bacteria including human pathogenic organism can either be by intercellular and extracellular interaction [44]. The most suggested machinery for antibacterial activity of the gold nanoparticles is by the oxidative stress on the microbial cell [45] leading to the formation of extracellular vacuole using the intracellular enzyme lactate dehydrogenase.…”

Section: Antibacterial Activitymentioning

confidence: 99%

Facile green synthesis of gold nanoparticles from marine algae Gelidiella acerosa and evaluation of its biological Potential

Senthilkumar¹,

Surendran²,

Sudhagar³

et al. 2019

SN Appl. Sci.

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In this present research, the Gold nanoparticles (GA-AuNPs) produced from Gelidiella acerosa, marine algae using green synthesis method was evaluated for its antidiabetic, antibacterial and antioxidant activity. Well-developed GA-AuNPs were effectively characterized using advanced analytical instruments like HRTEM, UV-visible, SEM and XRD. This analysis exposed GA-AuNPs to be present with spherical, hexagonal structure and crystalline nature. The average size of GA-AuNPs particles was estimated to lie between 5.81 nm to 117.59 nm by using Debye-Scherer equation. The enzyme inhibitory property of (IC 50 ) of G. acerosa aqueous extract and synthesized gold nanoparticles from G. acerosa against α amylase and α-glucosidase enzyme was found to be 2.1 ± 0.01, 3.7 ± 0.01 µg/mL and 2.8 ± 0.02, 4.1 ± 0.01, respectively. Further antioxidant assessment of GA-AuNPs were examined using DPPH and FRAP assays, the results exhibited that the strong antioxidant activity were observed in all the concentrations when compared with standard. Finally antibacterial activity was evaluated the GA-AuNPs against gram positive and gram negative bacteria, the strong antibacterial activity was observed in both the bacterial strains when compared with standard antibiotic. The GA-AuNPs from marine algae was exhibited to be strong inhibitory activity against alpha amylase and alpha glucosidase enzyme, antioxidant activity and antibacterial activity, these GA-AuNPs are projected to have potential biological applications.

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Section: Antibacterial Activitymentioning

confidence: 99%

Facile green synthesis of gold nanoparticles from marine algae Gelidiella acerosa and evaluation of its biological Potential

Senthilkumar¹,

Surendran²,

Sudhagar³

et al. 2019

SN Appl. Sci.

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“…16 Various nanoparticles such as silver, zinc, gold, zinc oxide (ZnO), iron oxide (Fe 3 O 4 ), titanium oxide (TiO 2 ), zirconium oxide (ZrO 2 ), and combinations of nanomaterials like chitosan/guanidine-functionalized graphene oxide, chitosan-iron oxide-coated graphene oxide have exhibited antimicrobial activity. [17][18][19][20][21][22][23][24] However, the mechanism of antimicrobial activity of these nanomaterials or nanomedicines is still debatable and their safe medical adoption requires detailed investigations on the biological entities. 25 Among many nanomaterials, silver nanoparticles (AgNps), known for their antimicrobial properties and higher microbial toxicity, have gained more impetus in biomedical and industrial applications than other metal nanoparticles.…”

Section: Introductionmentioning

confidence: 99%

Silver nanoparticles induced alterations in multiple cellular targets, which are critical for drug susceptibilities and pathogenicity in fungal pathogen (<em>Candida albicans</em>)

Radhakrishnan

Mudiam

Kumar

et al. 2018

IJN

129

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PurposeA significant increase in the incidence of fungal infections and drug resistance has been observed in the past decades due to limited availability of broad-spectrum antifungal drugs. Nanomedicines have shown significant antimicrobial potential against various drug-resistant microbes. Silver nanoparticles (AgNps) are known for their antimicrobial properties and lower host toxicity; however, for clinical applications, evaluation of their impact at cellular and molecular levels is essential. The present study aims to understand the cellular and molecular mechanisms of AgNp-induced toxicity in a common fungal pathogen, Candida albicans.MethodsAgNps were synthesized by chemical reduction method and characterized using UV–visible spectroscopy, X-ray powder diffraction, transmission electron microscopy, scanning electron microscopy–energy dispersive X-ray spectroscopy, energy dispersive X-ray fluorescence, and zeta potential. The anti-Candida activity of AgNps was assessed by broth microdilution and spot assays. Effects of AgNps on cellular and molecular targets were assessed by monitoring the intracellular reactive oxygen species (ROS) production in the absence and presence of natural antioxidant, changes in surface morphology, cellular ultrastructure, membrane microenvironment, membrane fluidity, membrane ergosterol, and fatty acids.ResultsSpherical AgNps (10–30 nm) showed minimum inhibitory concentration (minimum concentration required to inhibit the growth of 90% of organisms) at 40 μg/mL. Our results demonstrated that AgNps induced dose-dependent intracellular ROS which exerted antifungal effects; however, even scavenging ROS by antioxidant could not offer protection from AgNp mediated killing. Treatment with AgNps altered surface morphology, cellular ultrastructure, membrane microenvironment, membrane fluidity, ergosterol content, and fatty acid composition, especially oleic acid.ConclusionTo summarize, AgNps affected multiple cellular targets crucial for drug resistance and pathogenicity in the fungal cells. The study revealed new cellular targets of AgNps which include fatty acids like oleic acid, vital for hyphal morphogenesis (a pathogenic trait of Candida). Yeast to hypha transition being pivotal for virulence and biofilm formation, targeting virulence might emerge as a new paradigm for developing nano silver-based therapy for clinical applications in fungal therapeutics.

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“…It was described that about 90-95% of all bacterial colonies had decreased as result to AuNPs effective. Furthermore, the smallest NPs have the strongest antimicrobial effect compared to the larger sizes, which explained due to their ability to interact easily with bacterial cells, where bacteria have size about 50 to 5000 times bigger than NPs [74,75].…”

Section: Mechanism Of Penetration Of Npsmentioning

confidence: 99%

Comparison of Chemical and Biological Properties of Metal Nanoparticles (Au, Ag), with Metal Oxide Nanoparticles (ZnO-NPs) and their Applications

Abdussalam-Mohammed

2020

Adv. J. Chem. A

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Biological application of nanoparticles (NPs) is a rapidly developing area of nanotechnology, providing new possibilities in the diagnosis and treatment of human diseases. Nowadays, NPs have shown abilities to be used as alternative treatment for difficult diseases. Therefore, it is important to understand NP chemistry, preparation, interaction and possible mechanisms involved in its interaction with cancer cells and bacterial membranes. This study reports comparison between Au, Ag and ZnO NPs, and discusses their toxicity and physicochemical properties. In addition, this work aims to review different strategies of surface modification and functionalization of colloidal nanoparticles. Gold nanoparticles Silver nanoparticles Zinc oxide nanoparticles Toxicity growth Antibacterial activity Stability Capping agents

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Antibacterial effect of gold nanoparticles against Corynebacterium pseudotuberculosis

Abstract: Graphical abstract

Cited by 173 publications

References 20 publications

Facile green synthesis of gold nanoparticles from marine algae Gelidiella acerosa and evaluation of its biological Potential

Facile green synthesis of gold nanoparticles from marine algae Gelidiella acerosa and evaluation of its biological Potential

Silver nanoparticles induced alterations in multiple cellular targets, which are critical for drug susceptibilities and pathogenicity in fungal pathogen (<em>Candida albicans</em>)

Comparison of Chemical and Biological Properties of Metal Nanoparticles (Au, Ag), with Metal Oxide Nanoparticles (ZnO-NPs) and their Applications

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