Antibacterial efficacy, corrosion resistance, and cytotoxicity studies of copper-substituted carbonated hydroxyapatite coating on titanium substrate

Abstract

This work elucidated the antibacterial efficacy, corrosion resistance, and cytotoxicity of electroplated copper-substituted hydroxyapatite (CuHAP) coating on titanium (Ti). The fabricated CuHAP coatings were characterized by scanning electron microscopy, energydispersive X-ray analysis spectroscopy, Fourier transform infrared spectroscopy, and X-ray diffraction studies. The CuHAP coating had needle-like apatite crystals, the doping of Cu 2? into HAP reduced porosity, and the coating became denser. The CuHAP crystals were carbonated with a few of Cu 2? incorporation (about 0.80 wt%). The Cu 2? ions were homogenously deposited into HAP films. Potentiodynamic polarisation test revealed that the CuHAP coating provided good barrier characteristics and achieved superior corrosion protection for Ti substrates. The in vitro antibacterial activity of asprepared CuHAP coating was evaluated against Escherichia coli and was found to be effectively high against bacterial colonization. Bioactivity test conducted by soaking the coatings in simulated body fluid demonstrated that CuHAP coating can quickly induce bone-like apatite nucleation and growth. In vitro biocompatibility tests, MTT, were employed to assess the cytotoxicity of Cu-HAP coating with osteoblast-like MC3T3-E1 cells. The obtained HAP coating doped with a low content of Cu 2? exhibited good cytocompatibility and had no toxicity toward MC3T3-E1.

Surface Characterization and Biocompatibility of Selenium‐Doped Hydroxyapatite Coating on Titanium Alloy

“…As in its natural form, it is possible to incorporate different ions into the synthetic HA lattice. Based on this, scientists frequently study on doping and co‐doping of various ions into the structure of HA with the purpose of tailoring the properties of HA and therefore functionalizing implant surfaces and bone substitutes …”

Glancing Angle Deposition of Zn-Doped Calcium Phosphate Coatings by RF Magnetron Sputtering

“…It has been shown that the easiness of atomic doping or substitution in HA reveals its potential to be used in various clinical cases [4][5][6]. Most importantly, it has been shown that inorganic antibacterial metallic ions (e.g., Ag, Cu, Zn, Sr) can be introduced into the HA lattice and improve its antibacterial properties [7][8][9][10][11][12][13][14]. However, there is a physical approach to antibacterial surface production.…”

A Novel Approach for Accelerated Fabrication of Calcium Hydroxyapatite Thin Films

“…Many different techniques are being used to synthesize Calcium Hydroxyapatite (CHAp) coatings on the substrate [4]. Plasma spraying is the only one approved by the Food and Drug Administration (FDA) [5].…”