Antibacterial free-standing polysaccharide composite films inspired by the sea

Abstract

The adhesive capabilities of marine mussel proteins are well-known, exhibiting the ability to stick to different underwater substrates, either inorganic or organic. These unique adhesive properties are due to the high levels of amino acid, 3,4-dihydroxyphenyl-L-alanine (DOPA), which presents the reactive catechol group. Herein, novel antibacterial free-standing (FS) films were developed with natural polymers, namely chitosan (CHT) and hyaluronic acid (HA), being the catechol-functionalized hyaluronic acid (HA-DN) also included to provide wet adhesive properties. In order to obtain composite films, silver doped bioglass nanoparticles (Ag-BGs) were incorporated to promote bactericidal and bioactive properties, being tested four distinct formulations of FS films. Their surface morphology and topography, wettability, weight loss, swelling, mechanical, adhesion and bioactivity was analyzed. In particular, bioactivity tests revealed that upon immersion in simulated body fluid, there was the formation of a bone-like apatite layer. Moreover, upon 16 h in direct contact with Staphylococcus aureus and Escherichia coli cultures, these FS films exhibited a clear antibacterial effect. Therefore, such bioactive, antibacterial and adhesive free-standing films could potentially be used as temporary guided bone regeneration films, in particular to regenerate small bone defects and also periodontal tissues.

Spin-Coated Polysaccharide-Based Multilayered Freestanding Films with Adhesive and Bioactive Moieties

“…The storage modulus (E ) and the loss factor (E"/E ) as a function of the tensile loading frequency is presented in Figure 11. The results evidence an increase in E with the frequency increase for all the LbL films studied (with the exception of CTR50), a particular behavior of viscoelastic materials [66], being in agreement with other works [52,67]. Such increase was more pronounced for CatBG50, which also reached the highest E values (73.13 ± 5.6 MPa), suggesting that the synergetic effect of the catechol groups and the BGNPs' incorporation resulted in an increased film stiffness, reaching values 6.8 and 1.7 times higher than BG50 and Cat50, respectively.…”

Marine Polysaccharides for Wound Dressings Application: An Overview

“…Hyaluronic acid (HA), widely found in the extracellular matrix, is a naturally occurring acidic GAG. HA plays an essential role in inflammation, angiogenesis, and tumour microenvironment formation, which is therefore widely used in tissue engineering, soft tissue fillers, wound dressings, and other biomedical applications [87][88][89][90][91]. Sulphated GAGs, such as chondroitin sulphate, heparan sulphate, are found in the tissues of terrestrial and marine animals (e.g., intestinal mucosa, lungs, blood vessel walls, skin, bones, etc.)…”

Cotton fabric incorporated with β-cyclodextrin/ketoconazole/Ag NPs generating outstanding antifungal and antibacterial performances

“…However, none of those prepared samples with KZ showed antibacterial activity. Ag-NPs are sticking to microorganism membranes disrupting the function and making holes in the membrane and cytoplasmic uid out ow, causing cell death (Vale et al 2019;Wenhao et al 2020). Hence, Ag-NPs were synthesized on the β-CD/KZ composites to investigate their effects on the padded and exhausted samples.…”