Antibiofilm activity of quercetin-encapsulated cytocompatible nanofibers against <i>Candida albicans</i>

Vashisth, Priya; Nikhil, Kumar; Pemmaraju, Suma C; Pruthi, Parul A.; Mallick, Vivekanand; Singh, Harmeet; Patel, Alok; Mishra, Narayan Chandra; Singh, Rajesh; Pruthi, Vikas

doi:10.1177/0883911513502279

Cited by 43 publications

(30 citation statements)

References 31 publications

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“…Polymeric matrix provides an environment to the drug in which dispersed drug molecules can reside and experience the intermediate mobility and solubility as compare to pure components [11] . This in turn results into advanced pharmacokinetics properties as well as reduced side effects of drug [12] , [13] . A number of approaches have been introduced to formulate such systems which consists of biodegradable polymers along with antioxidant compound in order to improve the safety, therapeutic efficacy, stability and reduce toxicity by delivering the drug in a sustained manner for a prolong period [10] , [14] .…”

Section: Introductionmentioning

confidence: 99%

Biomedical applications of ferulic acid encapsulated electrospun nanofibers

Vashisth

Kumar

Sharma

et al. 2015

Biotechnology Reports

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Ferulic acid is a ubiquitous phytochemical that holds enormous therapeutic potential but has not gained much consideration in biomedical sector due to its less bioavailability, poor aqueous solubility and physiochemical instability. In present investigation, the shortcomings associated with agro-waste derived ferulic acid were addressed by encapsulating it in electrospun nanofibrous matrix of poly (d,l-lactide-co-glycolide)/polyethylene oxide. Fluorescent microscopic analysis revealed that ferulic acid predominantly resides in the core of PLGA/PEO nanofibers. The average diameters of the PLGA/PEO and ferulic acid encapsulated PLGA/PEO nanofibers were recorded as 125 ± 65.5 nm and 150 ± 79.0 nm, respectively. The physiochemical properties of fabricated nanofibers are elucidated by IR, DSC and NMR studies. Free radical scavenging activity of fabricated nanofibers were estimated using di(phenyl)-(2,4,6-trinitrophenyl)iminoazanium (DPPH) assay. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay confirmed the cytotoxicity of ferulic acid encapsulated nanofibers against hepatocellular carcinoma (HepG2) cells. These ferulic acid encapsulated nanofibers could be potentially explored for therapeutic usage in biomedical sector.

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Section: Introductionmentioning

confidence: 99%

Biomedical applications of ferulic acid encapsulated electrospun nanofibers

Vashisth

Kumar

Sharma

et al. 2015

Biotechnology Reports

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“…Because hypha and biofilm formation prevents cell death and apoptosis, a point where QS converges, strategies targeting these virulence factors to remove its inhibitory effect seem to be useful in overcoming the drug resistance of C. albicans. Suppression of hypha and biofilm formation and related genes has been demonstrated upon QCbased nanomaterial treatment in C. albicans (41). We therefore analyzed the effect of QC in FCZ-resistant NBC099 on the production of virulence factors.…”

Section: Fig 10mentioning

confidence: 99%

Quercetin Sensitizes Fluconazole-Resistant Candida albicans To Induce Apoptotic Cell Death by Modulating Quorum Sensing

Singh

Upreti

Singh

et al. 2015

Antimicrob Agents Chemother

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dQuorum sensing (QS) regulates group behaviors of Candida albicans such as biofilm, hyphal growth, and virulence factors. The sesquiterpene alcohol farnesol, a QS molecule produced by C. albicans, is known to regulate the expression of virulence weapons of this fungus. Fluconazole (FCZ) is a broad-spectrum antifungal drug that is used for the treatment of C. albicans infections. While FCZ can be cytotoxic at high concentrations, our results show that at much lower concentrations, quercetin (QC), a dietary flavonoid isolated from an edible lichen (Usnea longissima), can be implemented as a sensitizing agent for FCZresistant C. albicans NBC099, enhancing the efficacy of FCZ. QC enhanced FCZ-mediated cell killing of NBC099 and also induced cell death. These experiments indicated that the combined application of both drugs was FCZ dose dependent rather than QC dose dependent. In addition, we found that QC strongly suppressed the production of virulence weapons-biofilm formation, hyphal development, phospholipase, proteinase, esterase, and hemolytic activity. Treatment with QC also increased FCZmediated cell death in NBC099 biofilms. Interestingly, we also found that QC enhances the anticandidal activity of FCZ by inducing apoptotic cell death. We have also established that this sensitization is reliant on the farnesol response generated by QC. Molecular docking studies also support this conclusion and suggest that QC can form hydrogen bonds with Gln969, Thr1105, Ser1108, Arg1109, Asn1110, and Gly1061 in the ATP binding pocket of adenylate cyclase. Thus, this QS-mediated combined sensitizer (QC)-anticandidal agent (FCZ) strategy may be a novel way to enhance the efficacy of FCZ-based therapy of C. albicans infections. Candidiasis is a prevalent fungal infection caused by species of the yeast genus Candida. There are more than Candida 20 species, the most common of which is Candida albicans. Candidiasis is the fourth most common cause of health care-associated bloodstream infections in India and the United States (1). This yeast normally lives on the skin and mucous membranes without causing infection. However, overgrowth of these microbes can cause superficial mycoses, invasive mucosal infections, and disseminated systemic disease, particularly in AIDS patients, transplant recipients, and other immunocompromised people (2, 3). It is well documented that the emergence of drug-resistant C. albicans is increasing at an alarming pace (4-6). Therefore, the need for effective anticandidal therapy is increasing, as the available drugs are still very restricted.Currently available therapies for candidiasis are based on antifungal drugs including azoles, echinocandins, and inhibitors of calcineurin, Hos2 deacetylase, and Hsp90 (7-10). Fluconazole (FCZ) is most widely used to treat candidiasis infections because of its high bioavailability and low toxicity (11-13). However, excessive and indiscriminate clinical use of FCZ has led to the emergence of multiple-drug-resistant (MDR) strains of C. albicans (5,14,15). Importantly, t...

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“…When the surface tension of the polymer solution is overcome by the applied electric force, a fiber is extruded from the syringe tip (Huang et al 2003 ). This unique technique can produce the fibers with diameters ranging from nanometers to several microns (Vashisth et al 2013 ). In this investigation, two biodegradable polymers, PLGA and PEO were used as copolymer blend to fabricate electrospun nanofibers.…”

Section: Introductionmentioning

confidence: 99%

“…In this investigation, two biodegradable polymers, PLGA and PEO were used as copolymer blend to fabricate electrospun nanofibers. PLGA is FDA-approved biopolymer and widely used for different therapeutic applications (Vashisth et al 2013 ). However, due to its high cost of PLGA polymer, in this study, another biopolymer (PEO) was used along with it, to fabricate cost effective electrospun nanofibers.…”

Section: Introductionmentioning

confidence: 99%

Antiproliferative activity of ferulic acid-encapsulated electrospun PLGA/PEO nanofibers against MCF-7 human breast carcinoma cells

et al. 2014

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Ferulic acid (FA) is a polyphenolic phytonutrient which possesses strong antiproliferative effect; however, it has limited therapeutic applications due to its physiochemical instability and low bioavailability at the tumor site. In present study, these shortcomings associated with FA were overcome by fabricating FA-encapsulated poly(d,l-lactide-co-glycolide)/polyethylene oxide (PLGA/PEO) blend nanofibers using electrospinning technique. FESEM and fluorescence microscopic analysis imitates the smooth morphology and even distribution of FA within the polymeric nanofibers at optimum 2 wt% concentration of FA. The average diameters were recorded to be 150 ± 47.4 and 200 ± 79 nm for PLGA/PEO and FA-encapsulated PLGA/PEO nanofibers, respectively. The encapsulation, compatibility, and physical state of FA within the nanofibers were further confirmed by FTIR, TGA and XRD analysis. In vitro drug delivery studies demonstrated initial burst liberation of FA within 24 h followed by a sustained release for the subsequent time. MTT assay revealed the effectiveness of FA-encapsulated nanofibers against human breast carcinoma cells (MCF-7) cells as compared to control. FESEM and fluorescence microscopic analysis further confirmed the apoptotic effect of FA-encapsulated PLGA/PEO nanofibers against MCF-7. These fabricated nanofibers hold enormous potential to be used as a therapeutic agent for various biomedical applications.Electronic supplementary materialThe online version of this article (doi:10.1007/s13205-014-0229-6) contains supplementary material, which is available to authorized users.

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Antibiofilm activity of quercetin-encapsulated cytocompatible nanofibers against Candida albicans

Cited by 43 publications

References 31 publications

Biomedical applications of ferulic acid encapsulated electrospun nanofibers

Biomedical applications of ferulic acid encapsulated electrospun nanofibers

Quercetin Sensitizes Fluconazole-Resistant Candida albicans To Induce Apoptotic Cell Death by Modulating Quorum Sensing

Antiproliferative activity of ferulic acid-encapsulated electrospun PLGA/PEO nanofibers against MCF-7 human breast carcinoma cells

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